西藏牦牛、绵羊棘球蚴病病原的分子鉴定

doi:10.16431/j.cnki.1671-7236.2020.04.027

Abstract

Abstract: The purpose of this study was to identify the hydatid pathogen in yaks and sheep in Naqu and Lhasa of Tibet Autonomous Region and analyze its genetic variation.5 hydatid cysts were isolated from 5 sheep and 18 hydatid cysts from 15 yaks collected in Dangxiong county of Lhasa,and Jiali county of Naqu at the end of November 2016.Genomic DNA was extracted,nad1 gene was amplified by PCR,and nad1 full gene sequence was obtained by sequencing.DNAStar MegAlign software was used to analyze the sequence homology.Taking nad1 gene sequence of Echinococcus published in GenBank as comparison object,the phylogenetic tree was constructed by ML.The results showed that the nad1 gene sequence of 23 hydatid pathogens from yaks and sheep was highly homologous with that of the gene sequence of Echinococcus granulosus (G1 genotype),the homology was 99.6% to 99.8%,and the genetic distance of 23 nad1 genes was 0 to 0.0022447.The base variation rate of homologous genes was 0.2% to 0.4%,and that of homologous genes of Echinococcus was 14.9% to 19.8%.5 samples of nad1 gene had base mutation at different sites,and the mutation sites had sequence conversion.The results showed that the Echinococcus of yaks and sheep collected in this study were G1 genotype of Echinococcus granulosus,its nad1 gene had small variation and high sequence consistency.

Key words: Tibet; yak; sheep; hydatid cyst; molecular classification

CLC Number:

S855.9

XIA Chenyang, SHI Bin, TANG Wenqiang, Danqulamu, JIA Wanzhong, Bai Ji, WU Yantao, YAN Hongbin, LIU Jianzhi. Molecular Identification of Hydatid Cysts from Sheep and Yaks of Tibet[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1209-1215.

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Molecular Identification of Hydatid Cysts from Sheep and Yaks of Tibet

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