基因工程重组乳酸菌防控动物疫病的研究进展

doi:10.16431/j.cnki.1671-7236.2020.04.026

Abstract

Abstract: Most infectious pathogens enter the host through the mucosal surface.Therefore,besides the traditional vaccines that induce systemic immunity,mucosal vaccination should also be developed to establish the first line of defense at the mucosal invasion site to eliminate pathogens.Lactic acid bacteria are common probiotics in the intestines of humans and most animals,and have the functions of nutrition,immunity and anti-infection.Since 1990s,lactic acid bacteria have been used to express exogenous genes as food-grade carriers.The ability of lactic acid bacteria to elicit an immune response against expressed foreign antigens has led to their function as potential mucosal vaccine candidates.Lactic acid bacteria as vaccine vectors have many attractive advantages:Simple,noninvasive administration (usually oral or intranasal),the acceptance and stability of genetic modifications,expression of protein without purification,relatively low cost,and the highest level of safety possible,long-term survival in the body and continuous expression of foreign proteins,a single vaccination can produce a lasting immune response,and so on.At present,lactic acid bacteria as the normal bacterial group are selected as the carriers,and the recombinant lactic acid bacteria have been rapidly developed in many fields,such as,bacteria,viruses,parasites,etc.,and they have achieved significant effects in the corresponding disease models.This paper reviewed the preparation of genetically engineered recombinant lactic acid bacteria and the research progress in the prevention and control of animal diseases in the past five years.

Key words: lactic acid bacteria; genetic engineering; recombinant

CLC Number:

Q939.9

LIU Naizhi, QI Xiuye, GUO Yangli, CHENG Fuliang, XU Haiyan, GU Wei, SHAN Baolong, WANG Chunfeng. Research Progress of Genetic Engineering Recombinant Lactic Acid Bacteria on Epidemic Prevention and Control of Animal Diseases[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1199-1208.

References

[1] GOSALBES M J,ESTEBAN C D,GALON J L,et al.Integrative food-grade expression system based on the lactose regulon of Lactobacillus casei[J].Applied and Environmental Microbiology,2000,66(11):4822-4828.
[2] 杨洁彬,郭兴华.乳酸菌——生物学基础及应用[M].北京:中国轻工业出版社,1999. YANG J B,GUO X H.Lactic Acid Bacteria-Biological Basis and Application[M].Beijing:China Light Industry Press,1999.(in Chinese)
[3] 凌代文,东秀珠.乳酸菌分类鉴定及实验方法[M].北京:中国轻工业出版社,1999. LING D W,DONG X Z.The Taxonomic Identification of Lactic Acid Bacteria and Experimental Method[M].Beijing:China Light Industry Press,1999.(in Chinese)
[4] 蒋丹.FimH基因重组乳酸菌对鸡致病性大肠杆菌的保护作用研究[D].长春:吉林农业大学,2014. JIANG D.Research on the protect effect of FimH recombinant Lactobacillus on avian pathogenic Escherichia coli[D].Changchun:Jilin Agricultural University,2014.(in Chinese)
[5] BUKHARI S M.融合表达禽致病性大肠杆菌fimA-ompC基因及微皱褶细胞和树突状细胞靶向肽重组乳酸菌的鉴定及免疫学评价[D].哈尔滨:东北农业大学,2016. BUKHARI S M.Characterization and immunological evaluation of recombinant Lactobacillus expressing APEC fimA-ompC fusion protein with targeting peptide of microfold and dendritic cells[D].Harbin:Northeast Agricultural University,2016.(in Chinese)
[6] MA S T,DING G J,HUANG X W,et al.Immunogenicity in chickens with orally administered recombinant chicken borne Lactobacillus saerimneri expressing FimA and OmpC antigen of O78 avian pathogenic Escherichia coli[J].Journal of Medical Microbiology,2018,67(3):441-451.
[7] 杨一丹.pSIP-409-FaeG-DC重组乳酸菌对仔猪大肠杆菌腹泻的免疫保护作用研究[D].长春:吉林农业大学,2015. YANG Y D.The immune protective effect of the PSIP-409-FaeG-DC recombinant lactic acid bacteria on the Escherichia coli diarrhea piglets[D].Changchun:Jilin Agricultural University,2015.(in Chinese)
[8] 齐浩.ETEC 987P基因工程乳酸菌的制备及其免疫效果的检测[D].大庆:黑龙江八一农垦大学,2014. QI H.Immunogenicity of recombinant Lactobacillus casei expressing ETEC 987P gene[D].Daqing:Heilongjiang Bayi Agricultural University,2014.(in Chinese)
[9] LIU J K,WEI C H,HOU X L,et al.Passive protection of mice pups through oral or intranasal immunization of dams with recombinant Lactobacillus casei vaccine against ETEC F41[J].Research in Veterinary Science,2014,96(2):283-287.
[10] 葛俊伟,宋满满,赵鹏,等.产肠毒素大肠杆菌STa突变体、LTB和STb融合蛋白在乳酸菌中组成型分泌表达及其免疫原性分析[J].中国预防兽医学报,2017,39(12):1003-1007. GE J W,SONG M M,ZHAO P,et al.Secretory expression of mSTa,LTB and STb fusion protein of ETEC in Lactococcus lactis and the immunogenicity analysis[J].Chinese Journal of Preventive Veterinary Medicine,2017,39(12):1003-1007.(in Chinese)
[11] 宫旭颖.产气荚膜梭菌α-β2-ε-β1毒素融合基因在干酪乳杆菌中的表达及其免疫原性分析[D].哈尔滨:东北农业大学,2014. GONG X Y.Construction and expression of fusion protein of Alpha Beta2 epsilon Beta1 toxin of Clostridium perfringens in recombinant Lactobacillus casei and its immunogenicity analysis[D].Harbin:Northeast Agricultural University,2014.(in Chinese)
[12] 张艳.产气荚膜梭菌plc和NetB基因重组植物乳酸杆菌构建及其免疫保护研究[D].哈尔滨:东北农业大学,2014. ZHANG Y.Construction of recombinant Lactobacillus plantarum expressing plc and NetB gene of Clostridium perfringens and study on its immune protection[D].Harbin:Northeast Agricultural University,2014.(in Chinese)
[13] ALIMOLAEI M,GOLCHIN M,DANESHVAR H.Oral immunization of mice against Clostridium perfringens epsilon toxin with a Lactobacillus casei vector vaccine expressing epsilon toxoid[J].Infection Genetics and Evolution,2016,40:282-287.
[14] 刘鑫.牛分支杆菌ag85a基因重组乳酸菌的构建及免疫研究[D].长春:吉林农业大学,2015. LIU X.Construction and immunology research of recombinant Lactobacillus with ag85a gene of M.bovis[D].Changchun:Jilin Agricultural University,2015.(in Chinese)
[15] 陈敬蕊.牛分枝杆菌MPB83-70融合蛋白基因重组乳酸菌的构建及免疫研究[D].长春:吉林农业大学,2017. CHEN J R.Construction and immunology research of recombinant Lactobacillus with Mycobacterium bovis MPB83-70 fusion protein gene[D].Changchun:Jilin Agricultural University,2017.(in Chinese)
[16] 周建波.致羔羊腹泻奇异变形杆菌的分离鉴定及其ompA基因重组乳酸乳球菌口服疫苗研制[D].泰安:山东农业大学,2017. ZHOU J B.Isolation and identification of Proteus mirabilis from diarrhea lamb and development of recombinant Lactococcus lactis-expressing Proteus mirabilis ompA oral vaccine[D].Tai'an:Shandong Agricultural University,2017.(in Chinese)
[17] 周建波,胡莉萍,黄河,等.重组羊奇异变形杆菌ompA乳酸乳球菌的构建及其在小鼠体内的定植规律检测[J].中国兽医学报,2018,38(1):142-147. ZHOU J B,HU L P,HUANG H,et al.Construction of recombinant Lactococcus lactis-expressing sheep Proteus mirabilis ompA and detection of its colonization in mice[J].Chinese Journal of Veterinary Science,2018,38(1):142-147.(in Chinese)
[18] 杨文涛.靶向树突状细胞通用型禽流感病毒重组乳酸菌的研究[D].长春:吉林农业大学,2015. YANG W T.The study of dendritic cells targeting conserved AIVs antigen expressed by Lactobacillus plantarum NC8[D].Changchun:Jilin Agricultural University,2015.(in Chinese)
[19] 刘玉颖.pSIP409-NP-M1-DCpep乳酸菌制剂抗H9N2亚型禽流感效果的检测[D].长春:吉林农业大学,2016. LIU Y Y.Detection of the effects of pSIP409-NP-M1-DCpep Lactobacillus preparation against H9N2 avian influenza virus infection[D].Changchun:Jilin Agricultural University,2016.(in Chinese)
[20] YANG W T,YANG G L,SHI S H,et al.Protection of chickens against H9N2 avian influenza virus challenge with recombinant Lactobacillus plantarum expressing conserved antigens[J].Applied Microbiology and Biotechnology,2017,101(11):4593-4603.
[21] 李琼燕,杨桂连,张成赛,等.表达禽流感病毒NP和M2融合基因重组乳酸菌的构建及鉴定[J].中国兽药杂志,2018,52(12):9-15. LI Q Y,YANG G L,ZHANG C S,et al.Construction and identification of recombinant Lactobacillus plantarum NC8 expressing NP fused with M2 proteins of avian influenza virus[J].Chinese Journal of Veterinary Drug,2018,52(12):9-15.(in Chinese)
[22] 陈瑞玲.抗禽H9N2流感病毒树突状细胞靶向重组乳酸菌的构建及其免疫效果评价[D].长春:吉林大学,2016. CHEN R L.Construction of dendritic cell-targeted recombinant Lactobacillus against H9N2 avian influenza virus and evaluation of immune efficiency[D].Changchun:Jilin University,2016.(in Chinese)
[23] 赵亮.乳酸菌pSIP409-pgsA-3M2e-HA2的制备及其抗H9N2亚型禽流感免疫效果的研究[D].长春:吉林农业大学,2016. ZHAO L.Preparation of recombinant Lactobacillus pSIP409-pgsA-3M2e-HA2 and its immune effects against H9N2 subtype avian influenza virus[D].Changchun:Jilin Agricultural University,2016.(in Chinese)
[24] YANG W T,YANG G L,ZHAO L,et al.Lactobacillus plantarum displaying conserved M2e and HA2 fusion antigens induces protection against influenza virus challenge[J].Applied Microbiology and Biotechnology,2018,102(12):5077-5088.
[25] BO F,YANG W T,SHONYELA S M,et al.Immune responses of mice inoculated with recombinant Lactobacillus plantarum NC8 expressing the fusion gene HA2 and 3M2e of the influenza virus and protection against different subtypes of influenza virus[J].Virus Research,2019,263:64-72.
[26] 王棋,石昊林,王占楠,等.表面展示H9N2亚型禽流感病毒HA2蛋白的新功能型乳酸菌的构建[J].中国兽医科学,2017,47(4):476-480. WANG Q,SHI H L,WANG Z N,et al.Construction of novel functional lactic acid bacteria with surface displayed HA2 protein of H9N2 subtype AIV[J].Chinese Veterinary Science,2017,47(4):476-480.(in Chinese)
[27] 杨威.表面展示猪流感病毒NS1乳酸菌p SIP409-pgs A-NS1的制备及其免疫作用的研究[D].长春:吉林农业大学,2016. YANG W.Construction and research on immunological effects of Lactobacillus p SIP409-pgs A-NS1 with surface displayed swine influenza virus NS1[D].Changchun:Jilin Agricultural University,2016.(in Chinese)
[28] 刘琳琳.表达传染性法氏囊病病毒VP2重组乳酸菌主动递送活载体的构建及免疫效果评价[D].哈尔滨:中国农业科学院,2017. LIU L L.Construction of recombinant lactic acid bacteria active delivery vector expressing IBDV-VP2 and evaluation of immune effect[D].Harbin:Chinese Academy of Agricultural Sciences,2017.(in Chinese)
[29] 林红丽.干酪乳杆菌表面展示IBDV VP2蛋白及其免疫效力的研究[D].大庆:黑龙江八一农垦大学,2014. LIN H L.The surface of Lactobacillus casei expressing the VP2 from IBDV and research of immunogenicity[D].Daqing:Heilongjiang Bayi Agricultural University,2014.(in Chinese)
[30] 林红丽,侯申达,王嵩,等.表达鸡传染性法氏囊病毒VP2蛋白的干酪乳杆菌免疫保护效力[J].生物工程学报,2014,30(11):1679-1690. LIN H L,HOU S D,WANG S,et al.Immunogenicity of recombinant Lactobacillus casei expressing VP2 protein of infectious bursal disease virus in chickens[J].Chinese Journal of Biotechnology,2014,30(11):1679-1690.(in Chinese)
[31] IRAM M.组成型表达鸡传染性法氏囊病毒VP2基因重组乳酸菌的构建及免疫原性分析[D].哈尔滨:东北农业大学,2016. IRAM M.Construction of recombinant Lactobacillus constitutive expressing IBDV VP2 protein and analysis of its immunogenicity in chickens[D].Harbin:Northeast Agricultural University,2016.(in Chinese)
[32] 张旺,刘琳琳,祁小乐,等.表达鸡传染性法氏囊病毒VP2蛋白重组乳酸乳球菌的构建及其表达的优化[J].中国预防兽学报,2015,37(11):825-828. ZHANG W,LIU L L,QI X L,et al.Construction and optimization of recombinant Lactococcus lactis expressing avian infectious bursal disease virus VP2 protein[J].Chinese Journal of Preventive Veterinary Medicine,2015,37(11):825-828.(in Chinese)
[33] JIANG Y L,HU J T,GUO Y B,et al.Construction and immunological evaluation of recombinant Lactobacillus plantarum expressing HN of Newcastle disease virus and DC-targeting peptide fusion protein[J].Journal of Biotechnology,2015,216:82-89.
[34] 胡静涛.新城疫病毒HN基因重组乳酸菌抗小鼠结肠癌及免疫机制的初步研究[D].长春:吉林大学,2015. HU J T.Study on anti-tumor effect and immunity mechanism induced by NDV HN gene recombinant Lactobacillus on colon cancer-bearing BALB/c mice[D].Changchun:Jilin University,2015.(in Chinese)
[35] 王珊珊.干酪乳杆菌表达截短的IBV S1蛋白及其免疫原性的研究[D].大庆:黑龙江八一农垦大学,2018. WANG S S.Immunogenicity of the truncated IBV S1 protein surface-displayed on Lactobacillus casei[D].Daqing:Heilongjiang Bayi Agricultural University,2018.(in Chinese)
[36] 王胜华.J亚群禽白血病病毒GP85重组植物乳杆菌表达及其免疫效果的研究[D].泰安:山东农业大学,2017. WANG S H.Expression and immune effect of ALV-J Gp85 recombinant Lactobacillus plantarun in chicken[D].Tai'an:Shandong Agricultural University,2017.(in Chinese)
[37] 赵艳丽,柏亚铎,陈中秋,等.猪传染性胃肠炎病毒S1基因在食品级乳酸菌中的表达[J].中国兽医学报,2015,35(6):868-888. ZHAO Y L,BAI Y D,CHEN Z Q,et al.Expression of Sl gene of porcine transmissible gastroenteritis virus in food graded Lactococcus lactis[J].Chinese Journal of Veterinary Science,2015,35(6):868-888.(in Chinese)
[38] 王婧怡.猪传染性胃肠炎病毒抗体检测iELISA的建立及S1基因重组乳酸菌载体的构建[D].呼和浩特:内蒙古农业大学,2017. WANG J Y.Study of the indirect ELISA for detection of the antibody of porcine transminssible gastroenteritis virus and construction of the expression recombinants for S1 gene in Lactococcus lactis[D].Hohhot:Inner Mongolia Agricultural University,2017.(in Chinese)
[39] YANG W T,LI Q Y,ATA E B,et al.Immune response characterization of mice immunized with Lactobacillus plantarum expressing spike antigen of transmissible gastroenteritis virus[J].Applied Microbiology and Biotechnology,2018,102(19):8307-8318.
[40] 王莉平,韩先杰,王孝彬,等.表达猪流行性腹泻病毒S1蛋白的重组乳酸乳球菌的构建及其免疫原性的研究[J].中国兽医科学,2017,47(9):1118-1123. WANG L P,HAN X J,WANG X B,et al.Construction and immunogenicity of recombinant Lactococcus lactis expressing S1 protein of porcine epidemic diarrhea virus[J].Chinese Veterinary Science,2017,47(9):1118-1123.(in Chinese)
[41] 郭梦茹.新型猪流行性腹泻病毒S1基因重组乳酸菌载体的构建及小鼠免疫研究[D].长春:吉林农业大学,2018. GUO M R.Construction of recombinant Lactococcus lactis vector with S1 gene of variant porcine epidemic diarrhea virus and immunization in mice[D].Changchun:Jilin Agricultural University,2018.(in Chinese)
[42] HOU X Y,JIANG X P,JIANG Y P,et al.Oral immunization against PEDV with recombinant Lactobacillus casei expressing dendritic cell-targeting peptide fusing COE protein of PEDV in piglets[J].Viruses,2018,10(3):E106.
[43] 孙博.表达猪圆环病毒2型Cap蛋白重组乳酸乳球菌的构建及其免疫效果评价[D].哈尔滨:东北农业大学,2017. SUN B.Construction of recombinant Lactococcus lactis expressing Cap protein of porcine circovirus type 2 and evaluation of immunological effect[D].Harbin:Northeast Agricultural University,2017.(in Chinese)
[44] XU Y G,GUAN X T,LIU Z M,et al.Immunogenicity in swine of orally administered recombinant Lactobacillus plantarum expressing classical swine fever virus E2 protein in conjunction with thymosin α-1 as an adjuvant[J].Applied and Environmental Microbiology,2015,81(11):3745-3752.
[45] BHUYAN A A.表达牛病毒性腹泻病毒E2蛋白重组干酪乳杆菌的构建、免疫原性评价及其抗原捕获ELISA方法的建立[D].武汉:华中农业大学,2018. BHUYAN A A.Construction and immunogenicity evaluation of recombinant Lactobacillus casei expressing BVDV E2 protein and development of antigen capture ELISA for BVDV-1 detection[D].Wuhan:Huazhong Agricultural University,2018.(in Chinese)
[46] BHUYAN A A,MEMON A M,BHUIYAN A A,et al.The construction of recombinant Lactobacillus casei expressing BVDV E2 protein and its immune response in mice[J].Journal of Biotechnology,2018,270:51-60.
[47] 苏凤艳,李哲,李艳芝,等.重组犬瘟热病毒F-H融合基因乳酸菌的构建及表达[J].中国兽药杂志,2016,50(9):15-21. SU F Y,LI Z,LI Y Z,et al.Construction and expression of recombinant Lactobacillus containing F-H fusion gene of canine distemper virus from mink[J].Chinese Journal of Veterinary Drug,2016,50(9):15-21.(in Chinese)
[48] 赵维静,赵建伟,胡译文,等.表面展示狂犬病病毒糖蛋白的重组乳酸菌的构建及其免疫原性的研究[J].中国兽医科学,2019,49(4):432-439. ZHAO W J,ZHAO J W,HU Y W,et al.Construction and immunogenicity of recombinant lactic acid bacteria displaying rabies virus glycoprotein on the surface[J].Chinese Veterinary Science,2019,49(4):432-439.(in Chinese)
[49] LIU Y Y,YANG W T,SHI S H,et al.Immunogenicity of recombinant Lactobacillus plantarum NC8 expressing goose parvovirus VP2 gene in BALB/c mice[J].Journal of Veterinary Science,2017,18(2):159-167.
[50] SONG S S,LI P F,ZHANG R H,et al.Oral vaccine of recombinant Lactococcus lactis expressing the VP1 protein of duck hepatitis A virus type 3 induces mucosal and systemic immune responses[J].Vaccine,2019,37(31):4364-4369.
[51] 宋莎莎.表达3型鸭甲肝病毒VP1蛋白重组乳酸乳球菌株的构建及小鼠免疫试验[D].泰安:山东农业大学,2018. SONG S S.Construction of recombinant Lactococcus lactis expressing VP1 protein of duck hepatitis virus type 3 and immunity test on mice[D].Tai'an:Shandong Agricultural University,2018.(in Chinese)
[52] 高铭扬.表达鸡艾美耳球虫蛋白乳酸乳球菌免疫保护效果[D].哈尔滨:东北农业大学,2014. GAO M Y.Expression of chicken Eimeria 3-1e gene in Lactococcus lactis and its immune protection effect[D].Harbin:Northeast Agricultural University,2014.(in Chinese)
[53] 王芬.表达鸡柔嫩艾美耳球虫AMA1蛋白的乳酸乳球菌免疫保护作用[D].哈尔滨:东北农业大学,2016. WANG F.Protective effects of Lactococcus lactis expressing chicken Eimeria tenella AMA1 protein[D].Harbin:Northeast Agricultural University,2016.(in Chinese)
[54] LI J,WANG F,MA C L,et al.Recombinant Lactococcus lactis expressing Eimeria tenella AMA1 protein and its immunological effects against homologous challenge[J].Experimental Parasitology,2018,191:1-8.
[55] 周芳玉.表面展示柔嫩艾美耳球虫Et MIC2蛋白重组乳酸菌的构建及免疫保护效果研究[D].长春:吉林农业大学,2016. ZHOU F Y.Study on immune protective effect of recombinant lactic acid bacteria expressing Eimeria tenella Et MIC2 on its surface[D].Changchun:Jilin Agricultural University,2016.(in Chinese)
[56] 杨智勇.柔嫩艾美耳球虫SO7基因重组乳酸菌的构建及保护性效果研究[D].长春:吉林农业大学,2014. YANG Z Y.Construction and protective effect of immune-enhancing Lb.plantarum reconbing with SO7 gene of Eimeria[D].Changchun:Jilin Agricultural University,2014.(in Chinese)
[57] 宁红蕊.弓形虫GRA4、GRA7、GRA20、ROP20的序列分析及GRA4、GRA7重组乳酸菌的免疫效果[D].合肥:安徽农业大学,2016. NING H R.Sequence analysis of Toxoplasma gondii GRA4,GRA7,GRA20,ROP20 and immunopotency of recombinant Lactobacillus plantarum expressing GRA4 and GRA7[D].Hefei:Anhui Agricultural University,2016.(in Chinese)
[58] 倪晓婷.弓形虫ROP18和SAG1乳酸杆菌活载体疫苗株的构建及其免疫效果评价[D].兰州:甘肃农业大学,2016. NI X T.Construction of recombinant Lactobacillus plantarum expressing ROP18 and SAG1 and their protective efficacy against Toxoplasma gondii infection[D].Lanzhou:Gansu Agricultural University,2016.(in Chinese)
[59] BRON P A,TOMITA S,MERCENIER A,et al.Cell surface associated compounds of probiotic lactobacilli sustain the strain specificity dogma[J].Current Opinion in Microbiology,2013,16(3):262-269.
[60] FRANSEN F,ZAGATO E,MAZZINI E,et al.BALB/c and C57BL/6 mice differ in polyreactive IgA abundance,which impacts the generation of antigen-specific IgA and microbiota diversity[J].Immunity,2015,43(3):527-540.

Research Progress of Genetic Engineering Recombinant Lactic Acid Bacteria on Epidemic Prevention and Control of Animal Diseases

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	JIANG Haijun, WANG Dan, GUO Yu, WEI Li, HOU Lei, WANG Jing, ZHU Shanshan, LIU Jue. Fusion Expression and Immunogenicity of Recombinant Cap-flagellin Protein of Porcine Circovirus Type 3 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2044-2052.
[2]	CHEN Shaojun, LI Gang, NAI Zida, LIU Di, JIANG Xinpeng. Research and Application of Gene-edited Probiotics in Animal Intestinal Health [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1016-1024.
[3]	XIAO Li, NIU Xiaojie, LIU Qingqing, WANG Yueli, CHEN Chuangfu, YI Jihai. Construction of Recombinant Adenovirus with VP4 Gene of Porcine Rotavirus and Evaluation of Antibody Level [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 260-269.
[4]	LIU Jingsong, FU Jingcheng, WEN Bingyan, YANG Yanbin, GUO Shuang, JIAO Xianqin, CHEN Jin, HUANG Ruochao, WANG Yueying, LI Heping. Establishment and Analysis of Expression System of Porcine Intestinal Mucosal Protection Factor HO-1 in Bacillus pumilus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2064-2071.
[5]	SUN Yan, FENG Xiaowei, LIU Jiawei, LIU Bei, JIN Tianxiong, FENG Mingque, LIU Mingchao. Isolation,Identification and Antibacterial Activity of Lactic Acid Bacteria from Reproductive Tract of Healthy Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1852-1859.
[6]	LIU Qingqing, WU Peng, LI Peidong, ZHANG Jiangwei, CHEN Chuangfu, XIAO Chencheng. Construction of Recombinant Adenovirus Expressing Porcine Reproductive and Respiratory Syndrome Virus N Protein and Its Immunogenicity Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1024-1031.
[7]	SHANG Yu, LI Lintao, LI Li, XU Yingying, FENG Helong, WANG Hongcai, ZHANG Rongrong, ZENG Zhe, WANG Honglin, LUO Qingping, SHAO Huabin, WEN Guoyuan. Construction of Recombinant Heat-resistant Newcastle Disease Virus Expressing Fiber2 Protein of Fowl Adenovirus Serotype 4 and Evaluation of Its Immune Effect [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 650-659.
[8]	XU Ting, XIONG Ting, LI Linyu, LIU Yufu, WEN Lianghai, XIE Wenting, YANG Zekun, CHEN Ruiai. Construction and Identification of Recombinant Replication-deficient HAdV-5 Expressing VP2 Protein of Infectious Bursal Disease Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4524-4534.
[9]	WANG Zhenyu, TAN Zhongfang, WANG Weiwei, MA Hao, QIN Guangyong, WANG Yanping, PANG Huili. Research Progress on Aerobic Deterioration of Silage Feed and Its Preventive Measures [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4207-4217.
[10]	LI Shuang, DU Jiahua, BAI Zhihui, QU Ke, SU Miao, JIANG Ning, ZHANG Aizhong. Optimization of Lyophilized Combination for Pichia pastoris Containing Active Peptide CC34 Recombinant with Response Surface Methodology [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3791-3799.
[11]	ZHENG Huizhen, PU Hao, MIAO Ronghao, GAN Lu, GE Xiaomin, Bayinchahan, LI Yongchang, GUO Qingyong. Prokaryotic Expression and Bioinformatics Analysis of Theileria annulata enolase Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3972-3981.
[12]	WANG Xiangyan, XIN Guoqin, XU Haiyan, GU Wei, LAN Jianghua, HAO Muqiang, FENG Gang. Screening of a Strain of Pediococcus pentosus with High Antioxidant Activity [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 179-187.
[13]	YANG Mingzhu, CHEN Xiaochun, SONG Jiacheng, HOU Yaxin, LI Junping. Research Progress on Construction and Application of Recombinant Animal Herpesvirus Live Vector Vaccine [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 338-351.
[14]	JIANG Yajun, CHEN Shiyu, XIN Ting, GUO Xiaoyu, WANG Zhaoyang, LIU Xueting, CUI Shuai, WANG Yang, ZHU Hongfei, JIA Hong. Prokaryotic Expression of African Swine Fever Virus 360-12L Protein and Preparation of Its Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 352-360.
[15]	WANG Huan, ZHU Huabin, LI Junliang, ZOU Huiying, ZHAO Shanjiang. Targeted Editing of Hornless Pc Site in Holstein Bulls Using Tild-CRISPR/Cas9 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3343-3353.