Abstract:

The aim of the present study was to explore the genetic polymorphisms of myogenin (MyoG)gene in Cervidae. With the blood of 56 Gansu wapiti as experimental materials, PCR-SSCP and DNA sequencing approaches were applied to detect the single nucleotide polymorphisms (SNPs) and analyze the sequence variation at partial 5′UTR of MyoG gene. The results indicated that: ① The products amplified by primer 1 displayed polymorphism, three genotypes(AA, AB and BB) were detected in Gansu wapiti. The sequencing results showed that there was one single nucleotide mutation (G-237→A-237) at 5′UTR of MyoG gene. The polymorphism site was controlled by two alleles of A and B. Analysis of population genetics suggested that 〖WT〗〖ST〗〖HT〗〖HJ〗

〖WT5”BZ〗the genotype frequency of AA was the highest and A was the dominant allele gene. They were in low polymorphism (PIC=0.194) in PIC mutation site in 56 Gansu wapiti. There would be one Sp1 transcription factor missing at the mutation site by applying an analysis tool of TFSEARCH 1.3. ② Three genotypes (CC, CD and DD) determined by a SNP at -46 bp (G→A) were identified using primer 2, the genotype frequencies were in the order of CC＞CD＞DD, the PIC value (0.266) was intermediate polymorpgism (0.25＜PIC＜0.50). However, the potential elements and protein factors were not changed. This work would lay the foundation for further study on relationship between SNPs and carcass and meat quality traits in Gansu wapiti.

Key words: Gansu wapiti; myogenin gene; single nucleotide polymorphisms(SNPs); transcription factor