秦川牛PLAG1基因启动子克隆及转录因子预测

doi:10.16431/j.cnki.1671-7236.2023.07.007

Abstract

Abstract: 【Objective】 The purpose of this study was to explore the histological expression rules of pleomorphic adenoma gene 1 (PLAG1) in Qinchuan cattle, clone the promoter sequences of PLAG1 gene, predict and analyze the key transcription factor binding sites, so as to provide theoretical reference for exploring the transcriptional regulation mechanism of PLAG1 gene.【Method】 The relative expression of PLAG1 gene in the heart, liver, spleen, lung, kidney, subcutaneous fat, longissimus dorsi muscle and rumen of three 20-month-old Qinchuan adult bulls were detected by Real-time quantitative PCR, and the sequences of the upstream promoter region of PLAG1 gene were cloned.Bioinformatics software was used to predict PLAG1 gene transcription start sites and promoter core regions, and to analyze and screen key transcription factor binding sites in core promoter regions.【Result】 PLAG1 gene was expressed in all tissues of Qinchuan cattle, and the expression of PLAG1 gene in longissimus dorsi muscle was significantly higher than that in other tissues (P<0.05).The full length of the PLAG1 gene promoter sequence was 1 861 bp, and bioinformatics prediction analysis found that the core promoter region of the PLAG1 gene was located at -297-+42 bp. There were highly conserved transcription factor binding sites for Krüppel-like factor 5 (KLF5), cAMP-responsive element-binding protein-1 (CREB1) and early growth response factor 1 (EGR1), and the CpG island was located in the core promoter region of PLAG1 gene.【Conclusion】 PLAG1 gene was highly expressed in muscle tissue, and its core promoter region was located at -297-+42 bp.Transcription factors KLF5, CREB1 and EGR1 might have a regulatory effect on transcriptional activity of PLAG1 gene.The results provided theoretical basis for further investigation of PLAG1 gene transcriptional regulation mechanism.

Key words: Qinchuan cattle; PLAG1 gene; promoter; transcription factor

CLC Number:

S823

MA Yong, CHEN Zongchang, LI Xupeng, LI Jingsheng, BAI Yanbin, LIU Zhanxin, WEI Yali, GUO Dashan, SHI Bingang, ZHAO Zhidong, LUO Yuzhu, HU Jiang, WANG Jiqing, LIU Xiu, LI Shaobin. Cloning and Transcription Factor Prediction of PLAG1 Gene in Qinchuan Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2661-2669.

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Cloning and Transcription Factor Prediction of PLAG1 Gene in Qinchuan Cattle

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