荧光定量PCR检测牛性别相关基因DAX1表达的标准质粒和标准曲线的构建

›› 2010, Vol. 37 ›› Issue (9): 94-99.

• 生物技术 • Previous Articles Next Articles

Construction of Bovine Sex Related Genes DAX1 Standard Plasmid and Standard Curve by Real-time RT-PCR

XU Chao1，2，DU Wei-hua2， WANG Zong-li1，YU Da-wei2, WANG Dong2， HAO Hai-sheng2， 
ZHAO Xue-ming2， ZHU hua-bin2

(1.Grassland Research Institute，Chinese Academy of Agricultural Sciences，Hohhot 010010，China；2.Institute of Animal Sciences，Chinese Academy of Agricultural Sciences，Beijing 100193，China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20
Contact: WANG Zong-li

Abstract

Abstract: The primer was designed based on dosage-sensitive sex reversal adrenal hypoplasia critical region on chromosome X gene 1 （DAX1） according to the sequence which was submitted on GenBank. A recombinant plasmid contained cDNA fragment in DAX1 gene was constructed and used for the standard substance for quantitative detection of mRNA of the DAX1 gene in Holstein cows, a real-time PCR was developed for detection of DAX1 gene. The results revealed that this method had a good specificity, and the sensitivity was up to 102, range from 102 to 106. The cutoff value Ct had a good linear correlation with PCR samples. This method could be detected DAX1 gene in bovine quantitatively.

Key words: genital ridge; DAX1; real-time quantitative PCR; TaqMan probe; standard curve

CLC Number:

Q786

XU Chao;;DU Wei-hua;WANG Zong-li;YU Da-wei;WANG Dong;HAO Hai-sheng;ZHAO Xue-ming;ZHU hua-bin. Construction of Bovine Sex Related Genes DAX1 Standard Plasmid and Standard Curve by Real-time RT-PCR[J]. , 2010, 37(9): 94-99.

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Construction of Bovine Sex Related Genes DAX1 Standard Plasmid and Standard Curve by Real-time RT-PCR

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