一例猪圆环病毒2型和大肠杆菌、溶血葡萄球菌混合感染的诊断

China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (9): 220-222.

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Diagnosis of Mixed Infection of Porcine Circovirus 2,Escherichia coliand Staphylococcus haemolyticus

XU Hui1, YE Fei1,2, HE Yun-xia1,LI Fang-zheng3, GONG Yu-mei1, ZHANG Pei-jun1

(1.Institute for Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; 2.College of Life Science, Capital Normal University, Beijing 100037, China; 3.College of Animal Science,Qingdao Agricultural University,Qingdao 266109,China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20

Abstract

Abstract: A pig farm in Dezhou broke out disease that was similar to swine high fever disease. Samples of diseased pigs were collected. Total DNA and RNA of the samples were extracted. Porcine reproductive and respiratory syndrome virus(PRRSV), porcine circovirus type 2(PCV2), classic swine fever virus(CSFV),pseudorabies virus(PRV) and porcine parvovirus(PPV), were detected by PCR or RT-PCR. A 353 bp band which was specific for porcine circovirus type 2 was amplified. Further tests through bacterial isolation and culture, biochemical identification proved a mixed infection of PCV2, Escherichia coli and Staphylococcus haemolyticus.

Key words: porcine circovirus type 2; Escherichia coli; Staphylococcus haemolyticus; mixed infection; diagnosis

CLC Number:

S858.28

XU Hui;YE Fei;;HE Yun-xia;LI Fang-zheng;GONG Yu-mei;ZHANG Pei-jun. Diagnosis of Mixed Infection of Porcine Circovirus 2,Escherichia coliand Staphylococcus haemolyticus[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(9): 220-222.

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Diagnosis of Mixed Infection of Porcine Circovirus 2,Escherichia coliand Staphylococcus haemolyticus

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