新疆部分规模化牛场犊牛腹泻病毒性病原检测与流行特点分析

doi:10.16431/j.cnki.1671-7236.2025.03.029

Abstract

Abstract: 【Objective】 This study was aimed to understand the current epidemiological dynamic status of main viral pathogens that cause calf diarrhea in some large-scale farms in 4 dominant cattle production areas in Xinjiang,so as to provide epidemiological investigation basis for formulating precise prevention and control measures.【Method】 In this experiment,367 diarrhoeic anal swab samples were collected from some large-scale farms in prefectures of Bole,Ili,Kashgar and Changji of Xinjiang,5 diarrhea causing viruses,namely,Bovine viral diarrhea virus (BVDV),Bovine norovirus (BNoV),Bovine rotavirus (BRV),Bovine nebovirus (BNeV) and Bovine coronavirus (BCV) were detected by using PCR with the analysis of the difference between seasons,areas and the calf breeds.【Result】 The results showed that BVDV had the highest detection rate of 26.7%.A total of 11 mixed infections were detected,including 5 kinds of double infection,mainly BVDV+BNoV mixed infection (11.4%).There were 5 kinds of triple infection,mainly BVDV+BRV+BNoV mixed infection (3.5%).The quadruple infection was only BVDV+BRV+BNoV+BNeV mixed infection (0.2%).The detection rates of BVDV,BRV and BNoV in spring and summer were significantly higher than those in autumn and winter (P＜0.05),while the detection rates of BCV and BNeV were significantly lower than those in autumn and winter (P＜0.05).Five pathogens were detected in both Ili and Changji regions,with the highest detection rate of BVDV (92.3%) in Ili region,followed by BNoV (52.7%).The detection rate of BNoV was the highest (19.5%) in Changji area,followed by BVDV (17.8%).The detection rate of BNeV in dairy calves was higher than that in beef calves (P<0.05),and BNoV infection was predominant in dairy calves (18.6%).Beef calves were mainly infected with BVDV (31.1%).【Conclusion】 It was concluded that there were significant seasonal,regional and breeds differences in the infection of 5 calf diarrheic causing viruses in Bole,Ili,Kashgar and Changji of Xinjiang.The result of this study provided a scientific basis for comprehensive prevention and treatment of calf diarrhea in Xinjiang.

Key words: calf diarrhea; viral pathogen; mixed infection; virus detection

CLC Number:

S852.65⁺3

DING Ding, DING Xiaojun, MUSA Rehman, ZHEN Jie, SALAMAITI Srajiding, LI Na, SUN Yawei, CHEN Rulong, YAO Gang, MA Xuelian. Analysis on Viral Pathogen Detection and Epidemic Characteristics of Calf Diarrhea in Some Large-scale Cattle Farms in Xinjiang[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1272-1280.

References

[1] 赵颖婕.新疆南疆部分地区犊牛轮状病毒基因型鉴定及分析[D].阿拉尔:塔里木大学,2023. ZHAO Y J.Identification and analysis of calf Rotavirus genotypes in some areas of Southern Xinjiang[D].Alar:Tarim University,2023.(in Chinese)
[2] 汪梦竹,杨光美,吴玉湖,等.三株分离于新生小牛的牛病毒性腹泻病毒株的基因组特征[J].浙江农业学报，2023,35(8):1814-1822. WANG M Z,YANG G M,WU Y H,et al.Genomic features of 3 Bovine viral diarrhea viruses isolated from newborn claves[J].Zhejiang Journal of Agriculture,2023,35(8):1814-1822.(in Chinese)
[3] 赵逢淼,郭婷,周雅坪,等.牛病毒性腹泻病毒对新西兰白兔致病性分析及E2蛋白免疫效果的评价[J].中国畜牧兽医,2022,49(9):3549-3558. ZHAO F M,GUO T,ZHOU Y P,et al.Pathogenicity analysis of Bovine viral diarrhea virus and immune effect evaluation of E2 protein in New Zealand White rabbits[J].China Animal Husbandry & Veterinary Medicine,2022,49(9):3549-3558.(in Chinese)
[4] 秦义娴,刘丹,陈晓春.等.牛病毒性腹泻病毒检测方法研究进展[J].动物医学进展,2022,43(12):90-94. QIN Y X,LIU D,CHEN X C,et al.Progress on detection methods of Bovine viral diarrhea virus[J].Progress in Veterinary Medicine,2022,43(12):90-94.(in Chinese)
[5] 王珍贤.基于牛纽布病毒和牛诺如病毒VP1原核表达重组蛋白的两种间接ELISA方法的建立与应用[D].成都:西南民族大学,2021. WANG Z X.Establishment and application of two indirect ELISA methods based on prokaryotic expression of recombinantprotein of Bovine nebovirus and Bovine norovirus VP1[D].Chengdu:Southwest Minzu University,2021.(in Chinese)
[6] 魏其,屈勇刚,常军帅,等.新疆部分地区牛病毒性腹泻的流行病学调查[J].畜牧与兽医,2020,52(12):105-109. WEI Q,QU Y G,CHANG J S,et al.Molecular epidemiological investigation of bovine viral diarrhea in some areas of Xinjiang[J]. Animal Husbandry & Veterinary Medicine,2020,52(12):105-109.(in Chinese)
[7] 王玥琳,郭紫晶,岳华,等.部分地区奶牛腹泻粪便样中诺如病毒的检测和演化分析[J].畜牧兽医学报,2019,50(5):1048-1055. WANG Y L,GUO Z J,YUE H,et al.Detection and evolutionary analysis of Bovine noroviruses in diarrheic fecals of dairy calves in some areas[J].Acta Veterinaria et Zootechnica Sinica, 2019,50(5):1048-1055.(in Chinese)
[8] 马志刚,何琴,施晨阳,等.新疆地区GⅢ.2型牛诺如病毒流行病学调查及ORF1基因遗传进化分析[J].中国预防兽医学报,2022,44(8):888-891. MA Z G,HE Q,SHI C Y,et al.Epidemiological investigation and genetic evolution analysis of ORF1 gene of Bovine norovirus type GⅢ.2 in Xinjiang[J].Chinese Journal of Preventive Veterinary Medicine,2022,44(8):888-891.(in Chinese)
[9] 陆亚冬.犊牛冠状病毒和轮状病毒间接ELISA诊断方法的建立与初步应用[D].石河子:石河子大学,2018. LU Y D.The establishment and preliminary application of indirect ELISA for the diagnosis of calf Coronavirus and Rotavirus[D].Shihezi:Shihezi University,2018.(in Chinese)
[10] 于颖,周军,张斌.新疆地区奶牛6种腹泻相关病毒的检测[J].动物医学进展,2021,42(10):139-142. YU Y,ZHOU J,ZHANG B.Molecular detection of six diarrhea-associated pathogens in dairy cows in Xinjiang[J].Progress in Veterinary Medicine,2021,42(10):139-142.(in Chinese)
[11] 王梦娇,蒋倩,马学军,等.新疆地区牛冠状病毒的分子流行病学调查[J].畜牧兽医学报,2023,54(12):5125-5133. WANG M J,JIANG Q,MA X J,et al.Molecular epidemiological investigation of Bovine coronavirus in calf diarrhea in main cattle producing areas of Xinjiang[J].Acta Veterinaria et Zootechnica Sinica, 2023,54(12):5125-5133.(in Chinese)
[12] 陈超越,杭天宇,郭宇.等内蒙古包头市部分规模化牛场BRV流行病学调查[J].兽医导刊,2023,3:20-22. CHEN C Y,HANG T Y,GUO Y,et al.Epidemiological investigation of BRV in some large-scale cattle farms in Baotou[J]. Veterinary Orientation,2023,3:20-22.(in Chinese)
[13] 康台生,刘影,胡煜锋.等.牛冠状病毒牛肠病毒牛病毒性腹泻病毒三重一步法实时荧光PCR方法的建立与应用[J].中国兽医科学,2024,54(4):472-478. KANG T S,LIU Y,HU Y F,et al.Establishment and application of a one-step multiple fluorescence quantitative PCR method for detection of BCoV,BEV and BVDV[J].Chinese Veterinary Science,2024,54(4):472-478.(in Chinese)
[14] 米自由,欧武海,熊仲良.等.重庆市动物疫病定点流行病学调查技术规范[J].畜牧市场,2008,10:15. MI Z Y,OU W H,XIONG Z L,et al.Technical specifications for sentinel epidemiological survey of animal diseases in Chongqing[J].Stockbreeding Market,2008,10:15.(in Chinese)
[15] 刘枢清.动物疫病监测抽样技术规范[J].四川畜牧兽医,2022,49(4):55-56. LIU S Q.Technical specification for animal disease surveillance sampling[J].Sichuan Animal & Veterinary Sciences,2022,49(4):55-56.(in Chinese)
[16] SARRAZIN S,VELDHUIS A,MEROC E,et al.Serological and virological BVDV prevalence and risk factor analysis for herds to be BVDV seropositive in Belgian cattle herds[J].Preventive Veterinary Medicine,2013,108(1):28-37.
[17] 汪保,张星星,张宝刚,等.伊犁地区某肉牛场牛病毒性腹泻血清学调查[J].新疆农垦科技,2023,46(1):44-46. WANG B,ZHANG X X,ZHANG B G,et al.Serological investigation of bovine viral diarrhea in a cattle farm in Ili region[J].Xinjiang Farm Research of Science and Technology,2023,46(1):44-46.(in Chinese)
[18] 师志海,王文佳,兰亚莉.等.牛诺如病毒实时荧光定量PCR检测方法的建立及应用[J].畜牧兽医学报,2020,51(7):1728-1736. SHI Z H,WANG W J,LAN Y L,et al.Establishment and application of a Real-time PCR assay for detecting Bovine norovirus[J].Acta Veterinaria et Zootechnica Sinica,2020,51(7):1728-1736.(in Chinese)
[19] 曹竹亭,马素贞,高文斌.等.检测牛轮状病毒抗体间接ELISA方法的建立与应用[J].新疆农业大学学报,2008,5:73-77. CAO Z T,MA S Z,GAO W B,et al.Establishment of indirect ELISA to detect Bovine rotavirus antibody[J]. Journal of Xinjiang Agricultural University, 2008,5:73-77.(in Chinese)
[20] 崔鑫.新疆南疆部分规模奶牛场轮状和冠状病毒流行病学调查[D].阿拉尔:塔里木大学,2019. CUI X.Epidemiological survey of round and Coronavirus in some large-scale dairy farms in Southern Xinjiang[D].Alar:Tarim University,2019.(in Chinese)
[21] 李斯熠.我国部分省区牛纽布病毒的检测及其分子特征[D].成都:西南民族大学,2021. LI S Y.Detection and molecular characteristics of Bovine nebovirus in some provinces of China[D].Chengdu:Southwest Minzu University,2021.(in Chinese)

Analysis on Viral Pathogen Detection and Epidemic Characteristics of Calf Diarrhea in Some Large-scale Cattle Farms in Xinjiang

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