2016－2019年广西部分猪场仔猪病毒性腹泻的流行病学调查

doi:10.16431/j.cnki.1671-7236.2020.02.028

Abstract

Abstract: This study was aimed to understand main viral pathogens infection situation and popular characteristics of piglet diarrhea,and provide scientific basis for effective prevention and control of piglet diarrhea in Guangxi.In this experiment,RT-PCR and PCR methods were used to detect the pathogens of PEDV,PoRV,TGEV,PDCoV,CSFV,PRRSV,PCV2 and PRV of 914 diarrhea samples of piglets from 366 large-scale pig farms in 14 cities in Guangxi from 2016 to 2019,and analyzed these viruses pathogens mixed infection situation and the differences of the positive rates in different years,seasons and regions.According to the survey results,PEDV,PDCoV,PRRSV,PoRV,PCV2,CSFV and PRV all had different degrees of infection,and the average positive rate of the samples were 59.74%,8.32%,7.77%,4.92%,3.72%,3.28% and 2.08%,respectively,and no TGEV was detected.The PEDV had no significant seasonal variations,it was high all the year round.The PEDV still existed in infected piglet diarrhea,even in hot summer.However,the PDCoV had significant seasonal variations,it occurred frequently in both spring and winter.The positive rate of PEDV was high in Guangxi,the single infection rate was up to 50.55%.There were also many cases of diarrhea mixed infection in piglets,among which double infection was more common,and there were also four kinds of infection.The results showed that there were 7 kinds of viral pathogens infecting piglets in different degrees in Guangxi,among which the viral diarrhea caused by PEDV was particularly serious,and the positive rate of new PDCoV was second only to that of PEDV,so it was necessary to pay attention to and strengthen the prevention and control of new PDCoV.

Key words: piglet diarrhea; viral pathogen; epidemiology

CLC Number:

S858.28

DUAN Qunpeng, LI Xiaoyu, ZHAO Shuo, QIN Yibin, LU Bingxia, LI Bin, SU Qianlian, ZHAO Wu, HE Ying, LIANG Jiaxing, JIANG Dongfu, ZHOU Yingning, CHEN Zhongwei. A Epidemiological Investigation on Diarrhea Viral Disease of Piglets in Some Pig Farms in Guangxi During 2016 to 2019[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 564-574.

References

[1] SUN R Q,CAI R J,CHEN Y Q,et al.Outbreak of porcine epidemic diarrhea in suckling piglets,China[J].Emerging Infectious Diseases,2012,18(1):161-163.
[2] WANG X Y,CHENG J,XUE Z,et al.Infection,genetic and virulence characteristics of porcine epidemic diarrhea virus in Northwest China[J].Infection Genetics & Evolution,2018,62:34-39.
[3] 郭旋,陈静,刘欢,等.广西仔猪腹泻病毒病原流行病学调查[J].南方农业学报,2013,44(1):155-160. GUO X,CHEN J,LIU H,et al.Investigation on epidemiology of piglets diarrhea virus disease in Guangxi[J].Journal of Southern Agriculture,2013,44(1):155-160.(in Chinese)
[4] 王婧怡,申之义,原霖,等.我国部分地区原种猪场3种猪腹泻病毒的血清学调查[J].中国兽医学报,2018,38(2):255-258. WANG J Y,SHEN Z Y,YUAN L,et al.Serological investigation of three of viruses causing porcine diarrhea in pig breeding farms of selected areas in China[J].Chinese Journal of Veterinary Medicine,2018,38(2):255-258.(in Chinese)
[5] 秦谷雨,杨勇,李郁,等.安徽省仔猪腹泻5种病毒感染情况的调查研究[J].动物医学进展,2012,33(12):59-63. QIN G Y,YANG Y,LI Y,et al.Investigation on infection of five kinds of viruses causing pig diarrhea in Anhui province[J].Progress in Veterinary Medicine,2012,33(12):59-63.(in Chinese)
[6] SONG D,PARK B.Porcine epidemic diarrhoea virus:A comprehensive review of molecular epidemiology,diagnosis,and vaccines[J].Virus Genes,2012,44(2):167-175.
[7] JEOUNG H Y,LIM J A,JEONG W,et al.Three clusters of bovine kobuvirus isolated in Korea,2008-2010[J].Virus Genes,2011,42(3):402-406.
[8] 倪少闯,王添翔,杨晴,等.江苏某猪场仔猪腹泻的临床调查与病因分析[J].畜牧与兽医,2017,49(5):142-148. NI S C,WANG T X,YANG Q,et al.Clinical investigation and etiological analysis of piglets diarrhea at a pig breeding farm in Jiangsu province[J].Animal Husbandry & Veterinary Medicine,2017,49(5):142-148.(in Chinese)
[9] 刘艳成,杜雅楠,吴卫杰,等.内蒙古地区猪腹泻相关病毒的检测与分析[J].畜牧兽医学报,2015,46(9):1620-1628. LIU Y C,DU Y N,WU W J,et al.Detection an analysis of porcine diarrhea associated virus in Inner Mongolia[J].Acta Veterinaria et Zootechnica Sinica,2015,46(9):1620-1628.(in Chinese)
[10] 南文金,胡鸿惠,吴静波,等.粤北地区新生仔猪腹泻病原学调查及PEDV ORF3基因分子特征和遗传变异分析[J].中国畜牧兽医,2017,44(6):1769-1777. NAN W J,HU H H,WU J B,et al.Etiological investigation of diarrhea in newborn piglets and molecular characterization and genetic variation analysis of ORF3 gene of PEDV in North Guangdong[J].China Animal Husbandry & Veterinary Medicine,2017,44(6):1769-1777.(in Chinese)
[11] LI Z L,ZHU L,MA J Y,et al.Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field strains in South China[J].Virus Genes,2012,45(1):181-185.
[12] 王艳丰,张丁华,李爱心,等.PEDV、TGEV、PoRV 3种猪病毒性腹泻病毒的流行现状及控制策略[J].中国畜牧兽医,2018,45(2):518-527. WANG Y F,ZHANG D H,LI A X,et al.The prevalence and prevention of PEDV,TGEV and PoRV in porcine viral diarrhea[J].China Animal Husbandry & Veterinary Medicine,2018,45(2):518-527.(in Chinese)
[13] 郭晓秋,曲哲会,陈宏智,等.豫南地区规模化猪场仔猪腹泻病毒性病原感染情况调查[J].畜牧与兽医,2015,47(7):113-115. GUO X Q,QU Z H,CHEN H Z,et al.Investigation on diarrhea virus infection of piglets in large-scale pig farms in Southern Henan[J].Animal Husbandry & Veterinary Medicine,2015,47(7):113-115.(in Chinese)
[14] 李洋.一株猪繁殖与呼吸综合征病毒的分离鉴定及其基因组特性和致病性分析[D].杨凌:西北农林科技大学,2019. LI Y.Isolation and identification of a porcine reproductive and respiratory syndrome virus and analysis of its genomic characteristics and pathogenicity[D].Yangling:Northwest A & F University,2019.(in Chinese)
[15] 张若曦,张志,顾文源,等.河北省猪腹泻相关病毒的检测及猪流行性腹泻病毒S基因遗传变异分析[J].畜牧兽医学报,2018,49(3):597-605. ZHANG R X,ZHANG Z,GU W Y,et al.Detection of porcine diarrhea associated virus and genetic variation analysis of S gene of porcine epidemic diarrhea virus in Hebei province[J].Acta Veterinaria et Zootechnica Sinica,2018,49(3):597-605.(in Chinese)
[16] 关淼,赵晓彤,周晨阳,等.2016年辽宁规模化猪场病毒性腹泻流行状况调查分析[J].动物医学进展,2018,39(12):204-210. GUAN M,ZHAO X T,ZHOU C Y,et al.Prevalence investigation of swine viral diarrhea in large-scale pig farms in Liaoning area 2016 and genetic evolution analysis of PEDV S1 gene[J].Progress in Veterinary Medicine,2018,39(12):204-210.(in Chinese)
[17] 朱子健,闫丽辉,鞠妍,等.2015~2016年我国东北地区猪病毒性腹泻流行病学调查[J].中国预防兽医学报,2017,39(5):356-360. ZHU Z J,YAN L H,JU Y,et al.The epidemiological investigation on the viral diarrhea in pigs in the Northeast region of China during 2015-2016[J].Chinese Journal of Preventive Veterinary Medicine,2017,39(5):356-360.(in Chinese)
[18] 徐丽华,李军星,苏菲,等.2011-2017年浙江省猪病毒性腹泻病的流行病学调查[J].中国兽医科学,2018,48(5):625-630. XU L H,LI J X,SU F,et al.Epidemiological investigation on the viral diarrhea in pigs in Zhejiang province during 2011-2017[J].Chinese Veterinary Science,2018,48(5):625-630.(in Chinese)
[19] 王淞,曾昊,陈智,等.山东省猪腹泻病例中PEDV、TGEV和PRV的感染调查与分析[J].中国畜牧兽医,2017,44(7):2165-2170. WANG S,ZENG H,CHEN Z,et al.Investigation and analysis of porcine epidemic diarrhea virus,transmissible gastroenteritis virus and pseudorabies virus of swine diarrhea cases in Shangdong provine[J].China Animal Husbandry & Veterinary Medicine,2017,44(7):2165-2170.(in Chinese)
[20] 胡兴义,张双翔,金志强,等.贵州地区仔猪病毒性腹泻流行病学调查[J].中国预防兽医学报,2016,38(7):542-545. HU X Y,ZHANG S Y,JIN Z Q,et al.The epidemiological investigation on viral diarrhea of piglets in Guizhou region[J].Chinese Journal of Preventive Veterinary Medicine,2016,38(7):542-545.(in Chinese)
[21] 宋亚兵,徐帅飞,苏丹萍,等.2012年~2016年广东省猪丁型冠状病毒的流行病学调查及分析[J].中国预防兽医学报,2018,40(10):886-890. SONG Y B,XU S F,SU D P,et al.Epidemiological survey and analysis of porcine deltacoronavirus in Guangdong province from 2012-2016[J].Chinese Journal of Preventive Veterinary Medicine,2018,40(10):886-890.(in Chinese)
[22] 崔新格.河南省部分地区猪腹泻类冠状病毒流行病学调查及PEDV遗传进化分析[D].郑州:河南农业大学,2018. CUI X G.Epidemiological survey of swine diarrhea coronavirus in some areas of Henan province and analysis of its genetic evolution using PEDV[D].Zhengzhou:Henan Agricultural University,2018.(in Chinese)
[23] 秦毅斌,何颖,何苹萍,等.广西猪乙型脑炎血清流行病学调查分析[J].南方农业学报,2011,42(6):668-671. QIN Y B,HE Y,HE P P,et al.Serological epidemiology of Japanese encephalitis virus infection to swine in Guangxi[J].Southern Agricultural Journal, 2011,42(6):668-671.(in Chinese)
[24] 陈静.猪流行性腹泻的流行病学调查及免疫保护试验的研究[D].南宁:广西大学,2013. CHEN J.The study of PED epidemiological investigation and immunoprotection experiment of PED vaccines[D].Nanning:Guangxi University,2013.(in Chinese)
[25] 董波,张寄香,戴爱玲,等.闽西地区仔猪病毒性腹泻流行情况调查[J].江苏农业科学,2018,46(12):144-146. DONG B,ZHANG J X,DAI A L,et al.Epidemiological investigation and analysis of piglet viral diarrhea in Western Fujian[J].Jiangsu Journal of Agricultural Sciences,2018,46(12):144-146.(in Chinese)
[26] 朱良强,占松鹤,何长生,等.安徽部分地区冬春季仔猪腹泻的流行病学调查与分析[J].畜牧与兽医,2013,45(8):36-38. ZHU L Q,ZHAN S H,HE C S,et al.Epidemiological investigation and analysis of piglet diarrhea in Anhui province[J].Animal Husbandry & Veterinary Medicine,2013,45(8):36-38.(in Chinese)
[27] 林庆燕,陈书琨,孙洁,等.引起猪腹泻的病毒分离鉴定[J].动物医学进展,2014,35(10):75-79. LIN Q Y,CHEN S K,SUN J,et al.Isolation and identification of porcine diarrhea viruses[J].Progress in Veterinary Medicine, 2014,35(10):75-79.(in Chinese)
[28] 秦毅斌,卢冰霞,何颖,等.猪流行性腹泻病毒CH/GX/2015/750A株的分离鉴定及全基因组序列分析[J].中国畜牧兽医,2018,45(1):178-188. QIN Y B,LU B X,HE Y,et al.Isolation,identification and complete genome sequence analysis of porcine epidemic diarrhea virus CH/GX/2015/750A[J].China Animal Husbandry & Veterinary Medicine,2018,45(1):178-188.(in Chinese)
[29] 秦毅斌,何苹萍,卢冰霞,等.猪丁型冠状病毒CH/GX/1468B/2017的分离鉴定及全基因组序列分析[J].中国畜牧兽医,2019,46(7):1907-1916. QIN Y B,HE P P,LU B X,et al.Isolation,identification and complete genome sequence analysis of porcine deltacoronavirus strain CH/GX/1468B/2017[J].China Animal Husbandry & Veterinary Medicine,2019,46(7):1907-1916.(in Chinese)

A Epidemiological Investigation on Diarrhea Viral Disease of Piglets in Some Pig Farms in Guangxi During 2016 to 2019

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	KANG Pu, REN Zhaowen, CAI Rujian, LYU Zongji, HUANG Yuhao, WANG Xiaohu. Variation Regularity and Transmission of PRRSV-2 Lineage 8 Strains in Guangdong and Surrounding Provinces [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2001-2011.
[2]	YANG Zhi, YU Jiaxin, YANG Linfu, WANG Kang, LI Guanzong, DUAN Gang, YANG Fumei, LIU Zhiqi, ZHOU Ke, HE Jun, DAI Feiyan. Study on the Therapeutic Effect of Modified Sini Decoction on Piglet Diarrhea [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1707-1717.
[3]	LIANG Yingxin, CHEN Zejia, LI Jichao, CUI Xiaoxiao, FENG Qianqi, LI Shoujun, CHEN Aolei. Investigation on the Infection of Equine Intestinal Parasite in Partial Cities in Guangdong Province [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2725-2734.
[4]	GUO Luanying, LI Jialei, JIN Qinqin, MA Jun, LIU Quan. Research Progress on Bovine Hepacivirus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1994-2000.
[5]	CHEN Wenguang, ZHANG Yang, XIAO Xiaoyue, LIU Yanyan, CHEN Xueying, WANG Xiumin, QIN Junjie, LI Yanhua. Investigation of the Effective Components and Active Mechanism of Qiweng Huangbo Powder in the Treatment of Piglet Diarrhea Based on Network Pharmacology [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4898-4908.
[6]	WU Jing, SHI Jincheng, SANIYE Kerban, NAMAN Abudula, SUN Lei, YAO Gang, ZHONG Qi, LUO Liangtao, ZHAO Hongqiong, MA Xuelian. Investigation of the Main Viral Pathogens of Angus Calf Diarrhea in Kashgar Region of Xinjiang and Whole Gene Sequence Analysis of Bovine Norovirus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4420-4428.
[7]	YIN Yanwen, SHI Kaichuang, SUN Wenchao, XIE Shouyu, QU Sujie, LU Wenjun, WANG Luxia, QIN Yong, PEI Xingbiao, LING Dan. Surveillance Analysis of Low Pathogenic Avian Influenza Virus in Border Areas of Guangxi from 2013 to 2019 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 3002-3009.
[8]	ZHANG Shuang, GUO Yu, XUE Wenhui, ZHAO Qianhui, HUO Shuying. Research Progress on Poultry Candida glabrata [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1013-1019.
[9]	SUN Jiali, DONG Hao, PENG Xiaowei, ZHU Liangquan, JIANG Hui, FENG Yu, LI Xiaoying, DING Jiabo, WANG Chunfeng. Research Progress on Brucella canis [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3803-3811.
[10]	WANG Jiayun, WANG Xin, WANG Bingyu, ZENG Hao, SHEN Wenhui, YU Jiang, CHEN Zhi, ZHANG Yuyu, SUN Wenbo, GUO Lihui, REN Sufang, LI Yubao, WU Jiaqiang. Epidemiological Investigation of CSFV in Shandong Province from 2017 to 2019 [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1870-1881.
[11]	CHEN Qian, WANG Kunlun, YAN Yaqun, ZHOU Yongchun, ZHAO Cong, JIAN Fuchun, WANG Rongjun, ZHANG Longxian, NING Changshen. Molecular Epidemiological Investigation and Genetic Evolution Analysis of Theileria luwenshuni in Sheep and Goats in Different Regions of China [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1902-1909.
[12]	LIU Hanyang, ZHANG Yi, XIAO Ran, CHEN Bin, WU Yongsheng, SU Zhipeng, LI Yupeng, PEI Chaoxin, XU Zhenying, ZHU Jiawen. Molecular Epidemiological Investigation on ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus in Sichuan in 2018 [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1172-1182.
[13]	DUAN Qunpeng, ZHAO Shuo, QIN Yibin, LU Bingxia, HE Ying, CHEN Zhongwei, LIU Lei, ZHOU Yingning, LI Bin, JIANG Dongfu, LIANG Jiaxing, ZHAO Wu. Epidemiological Investigation Analysis of Porcine Circovirus Type 3 in Guangxi [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1183-1190.
[14]	YANG Chunjie, LIU Xueting, MI Xue, CHEN Ying, WEI Zuzhang, HUANG Weijian, OUYANG Kang. Epidemiological Investigation and VP1 Gene Sequence Analysis of Porcine Enterovirus Type G in Guangxi [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1216-1224.
[15]	LI Shaohan, ZHANG Guangzhi, CUI Shangjin, QIN Tong. Research Progress of Canine Coronavirus and Feline Coronavirus [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 4059-4068.