基于网络药理学探究芪翁黄柏散治疗仔猪腹泻的有效成分及作用机制

doi:10.16431/j.cnki.1671-7236.2022.12.037

Abstract

Abstract: 【Objective】 The purpose of this research was to predict the effective components and explore the mechanism of Qiweng Huangbo powder in treating diarrhea of piglets based on network pharmacology.【Method】 Potential active ingredients and target proteins of Qiweng Huangbo powder were screened through TCMSP database website, and Cytoscape 3.9.1 software was used to construct a drug components-targets network.GeneCards and OMIM databases were used to retrieve and remove repeat targets, and the common targets of disease and drugs were obtained.The common targets were imported into STRING database and the protein-protein interaction (PPI) network was constructed.GO function and KEGG pathway enrichment analysis were performed for the common targets.【Result】 A total of 37 active components and 74 targets of Qiweng Huangbo powder were obtained, among which 68 common targets intersected with the disease.147 biological process, 15 cell component and 32 molecular function were obtained by GO functional annotation.98 pathways were obtained by KEGG pathway enrichment analysis.Quercetin, kaempferol, stigmasterol, tetrahyroberberine, berberine and so on were active components.Tumour necrosis factor (TNF), tumor protein p53(TP53), vascular endothelial growth factor A(VEGFA), interleukin 6 (IL6), IL10, chemokine 2(CCL2), epidermal growth factor (EGF), NF-kappa-B inhibitor alpha(NFKBIA) and so on were shown as key targets of the drug.Cancer, phosphatidylinositol-3/kinase protein kinase B(PI3K-Akt), mitogen-activated protein kinase (MAPK), IL17, inflammatory bowel disease and other signal pathways were the key pathways, and which also involved in immune response, transcription, apoptosis, cytokine activity, extracellular aspects.【Conclusion】 This study predicted the effective components and action mechanism of Qiweng Huangbo powder in the treatment of piglet diarrhea, which reflected the characteristics of multiple components, multiple targets and multiple pathways of Traditional Chinese medicine, and provided direction and reference for subsequent research.

Key words: network pharmacology; piglet diarrhea; Qiweng Huangbo powder; signal; pathways

CLC Number:

S853.74

CHEN Wenguang, ZHANG Yang, XIAO Xiaoyue, LIU Yanyan, CHEN Xueying, WANG Xiumin, QIN Junjie, LI Yanhua. Investigation of the Effective Components and Active Mechanism of Qiweng Huangbo Powder in the Treatment of Piglet Diarrhea Based on Network Pharmacology[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4898-4908.

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Investigation of the Effective Components and Active Mechanism of Qiweng Huangbo Powder in the Treatment of Piglet Diarrhea Based on Network Pharmacology

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