100 SD rats weighting 100～120 g were divided randomly into 3 groups. The animals in groupⅠwere fed with 3.0 mg/kg selenium as Se-enriched malt (SEM) supplemented diets. Animals in groupⅡand groupⅢreceived 0.1 mg/kg selenium in sodium selenite only and were severed as the positive control and negative control respectively. Animals groupⅠand groupⅡwere induced hepatoma by diethylnitrosamine resolved 〖JP+3〗with sterilized water (100 mg/L) at the dosage of 10 mg/kg body weight every day for 16 weeks，then drank with sterilized water for another 2 weeks. At 18th week，the rats were killed. Microvascular density (MVD) and.vascular endothelial growth factor (VEGF) protein level in tumor tissue were determined by immunohistochemistry. The results indicated that MVD and VEGF protein levels in the SEM group were significantly lower than those in the model group (P<0.05). It demonstrated that SEM could inhibit angiogenesis in the rat hepatoma. The down-regulating of VEGF expression might be involved in the angiogenesis inhibition.

In this study，mRNA isolated from embryos was subjected to reverse transcription polymerase chain reaction to investigate the expression of eNOS, iNOS mRNA in normal pathenogenetic activation porcine embryos. The results showed that eNOS was expressed only in two-cell and four-cell stage embryos, while iNOS was expressed around early embryo development stages. These studies determined that inducible NOS (iNOS) and endothelial NOS (eNOS) are differentially distributed in cells along the development, higher level of eNOS was detected at four-cell stage embryo but iNOS at morula/blastocyst stage. The study provides the first evidence for the presence of mRNA of NOS isoforms during porcine embryogenesis. Furthermore, the expression of NOS during the embryo development suggest that NO may be of crucial importance for reproductive events that occur in this period.

Effect of four commonly used immunization program of Newcastle disease in commercial broiler were detected under the high maternal antibody titer, the results indicated that the level of maternal antibody had decreased significantly, the change was not significant in group but significant between groups. After the second inoculation, maternal antibody still had great interference with the live vaccine group, the antibody titer below the protection level(6 log2) at 55 days old, but the antibody titer of the 7-day-old group higher than 3-day-old group. Maternal antibody had small interference with the group used both inactivated vaccine and live vaccine, antibody level of 3-day-old group up to 6log2 at 50 days old while antibody level of 7-day-old group up to 6.4log2 at 45 days old and rose to 7log2 at 50 days old and maintained to the end of trial. The antibody level of 7-day-old group which was about five days ahead of schedule to achieve the level of antibody protection was higher than the 3-day-old group.

Immune colloidal gold technique has been received universal attention by the domestic and foreign researchers for its fast, accurate and simple characteristic. The main affect factors of the technique were analysised in this research， the materials and the methods for preparing and labelling the colloidal gold, coating the nitrocellulose filter, the preservation and stability detection for the end item. It is expected to provide the theory basis and the technology reference for preparing the production of the colloidal gold detection.