Abstract: In broiler-fryer industry of Chongqing, there has a disease which harmed 15 to 30 days old broilers since September 2007. The disease occurred as an acute septicemia characterized by fibrinous perricarditis, perihhepatitia, and airsacculitis. In order to search for the etiopathogenisis and the means of cure, we isolated and identified the pathogenic bacterium. In the study, SS nutrient agar was used, and 26 strains Salmonella enteritidis (SE) were isolated and identified by biochemistry and PCR. The SE strains had pathogenicity for chicken, ducklings and mice. Drug susceptibility test for 26 strains showed their resistance to Amoxicillin, Ofloxacin, and so on. The chicken immunized with CQ0709 strain inactivated vaccine or injected with special IgY can against artificial challenge. The results showed drug resistance SE infection had been a problem in the intensive production of broiler in Chongqing.

Key words: broiler;  Salmonella enteritidis; drug susceptibility test; PCR