新疆阿克苏地区羊泰勒虫PCR检测及遗传多样性分析

doi:10.16431/j.cnki.1671-7236.2025.03.031

Abstract

Abstract: 【Objective】 The purpose of this experiment was to investigate the classification,genetic diversity and incidence of Theileria of sheep in Keping and Aatii counties,Aksu region,Xinjiang.【Method】 A total of 78 sheep blood samples from farms in Keping and Awati counties were tested for the pathogen of ovine theileriosis by PCR,and the positive samples were sequenced,and the sequences obtained were analyzed for similarity and genetic diversity with 18S rRNA sequences of Theileria species in GenBank.【Result】 The overall PCR positive rate of ovine theileriosis was 84.6% (66/78) in the two counties,with rates of 73.3% (22/30) in Keping county and 91.7% (44/48) in Awati county.The similarity of 6 representative sequences obtained by sequencing was more than 93%,and their similarity with 18S rRNA sequences of Theileria ovis in GenBank database was more than 97%.The genetic evolution tree revealed independent clades for 9 species of Theileria ovis,Theileria annulata and Theileria cervi,and so on.Theileria ovis was closely related to Theileria annulata,and was closely related to isolates from Turkey,Iraq and Saudi Arabia.Haplotype analysis of 50 18S rRNA gene sequences from seven countries including China,Iraq,Turkey,Saudi Arabia showed that these sequences were distributed in 16 haplotypes.Five sequences from this study were grouped into Hap_1 along with sequences from Henan and Gansu provinces in China,and the other one was isolated as Hap_16.Population structure analysis using a total of 47 sequences from China,Iraq,Turkey and Saudi Arabia indicated a separation site in all four countries.Strains from Iraq exhibited high nucleotide diversity,while that of isolates from China,Turkey and Saudi Arabia was low.Strains from Turkey had the highest average difference number,indicating large genetic differences within the population compared to the other three countries where genetic differences were small.Haplotype diversity for isolates from Turkey,Iraq and Saudi Arabia was more than 0.5,with Iraqi isolates showing the highest value reflecting high genetic diversity,whereas Chinese strain value was 0.250 indicating low genetic diversity.Tajima’s D value for all four countries above were less than 0,suggesting that the population had shifted its genetic balance state,possibly due to population expansion.【Conclusion】 The data of this study showed that Theileria infection was prevalent in Keping and Awali counties,Aksu,and Theileria ovis was the main pathogen. Theileria uilenbergi and Theileria luwenshuni were not detected,and Theileria ovis had a distant genetic relationship with them.Theileria ovis populations from different regions were characterized by high haplotype and genetic diversity,and population expansion was occurring.The results of this study could provide a reference for the comprehensive control of ovine theileriosis and the healthy development of local aquaculture in Aksu.

Key words: sheep; Theileria; PCR detection; genetic evolution; prevention and control

CLC Number:

S852.72

ZHOU Na, LI Caishan, ZHAO Xueqing, ABUDUKADIER· Mejiti, DENG Yuqian, LIU Shiyu, SHI Wenyu, GUO Qingyong, BAYINGCHAHAN· Gailike. PCR Detection and Genetic Diversity Analysis of Theileria of Sheep in Aksu,Xinjiang[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1292-1300.

References

[1] AL-HOSARY A A,ELSIFY A,SALAMA A A,et al.Phylogenetic study of Theileria ovis and Theileria lestoquardi in sheep from Egypt:Molecular evidence and genetic characterization[J].Veterinary World,2021,14(3):634-639.
[2] 陈倩,王坤轮,闫亚群,等.中国不同地区羊吕氏泰勒虫分子流行病学调查及遗传进化分析[J].中国畜牧兽医,2020,47(6):1902-1909. CHEN Q,WANG K L,YAN Y Q,et al.Molecular epidemiological investigation and genetic evolution analysis of Theileria luwenshuni in sheep and goats in different regions of China[J]. China Animal Husbandry & Veterinary Medicine, 2020,47(6):1902-1909.(in Chinese)
[3] MORRISON W I.The aetiology,pathogenesis and control of theileriosis in domestic animals[J].Revue Scientifique et Technique,2015,34(2):599-611.
[4] EL IMAM A H,HASSAN S M,GAMEEL A A,et al.Variation in susceptibility of three Sudanese sheep ecotypes to natural infection with Theileria lestoquardi[J].Small Ruminant Research,2015,124:105-111.
[5] YAGHFOORI S,MOHRI M,RAZMI G.Experimental Theileria lestoquardi infection in sheep:Biochemical and hematological changes[J].Acta Tropicaica,2017,173:55-61.
[6] 郝桂英,杨丽静,李瑞琪,等.四川省攀西地区羊梨形虫的分子流行病学调查[J].中国预防兽医学报,2021,43(1):92-97. HAO G Y,YANG L J,LI R Q,et al.Molecular epidemiological survey of ovine and caprine infection with piroplasms in Panxi region of Sichuan province[J].Chinese Journal of Preventive Veterinary Medicine,2021,43(1):92-97.(in Chinese)
[7] 李有全,彭欲率,刘志杰,等.中国部分地区羊泰勒虫病的流行病学调查及分类鉴定[J].中国农业科学,2012,45(16):3422-3429. LI Y Q,PENG Y S,LIU Z J,et al.Epidemiological survey and identification of Theileria parasite infection for small ruminants in some parts of China[J].Scientia Agricultura Sinica,2012,45(16):3422-3429.(in Chinese)
[8] 王铁男,胡诗悦,钱年超,等.吉林省延边地区羊泰勒虫病的分子流行病学调查[J].中国预防兽医学报,2013,35(12):1020-1022. WANG T N,HU S Y,QIAN N C,et al.Molecular epidemiological of ovine and caprine theileriosis in Yanbian area[J].Chinese Journal of Preventive Veterinary Medicine,2013,35(12):1020-1022.(in Chinese)
[9] NEHRA A K,MOUDGIL A D,KUMARI A,et al.Population genetic characterization of Theileria annulata based on the cytochrome b gene,with genetic insights into buparvaquone susceptibility in Haryana (India)[J].Acta Tropica,2024,250:107103.
[10] 李有全.我国羊泰勒虫的生物学特性研究[D].北京:中国农业科学院,2007. LI Y Q.Biological characterization of Theileria spp.infective for sheep and goats in China[D] Beijing:Chinese Academy of Agricultural Sciences, 2007.(in Chinese)
[11] 刘国平,任清明,贺顺喜,等.我国东北三省蜱类的分布及医学重要性[J].中华卫生杀虫药械,2008,14(1):39-42. LIU G P,REN Q M,HE S X,et al.Distribution and medical importance of ticks in three provinces of Northeast China[J].Chinese Journal of Hygienic Insecticides & Equipments, 2008,14(1):39-42.(in Chinese)
[12] OOSTHUIZEN M C,ALLSOPP B A,TROSKIE M,et al.Identification of novel Babesia and Theileria species in South African giraffe (Giraffa camelopardalis,Linnaeus,1758) and roan antelope (Hippotragus equinus,Desmarest 1804)[J].Veterinary Parasitology,2009,163(1-2):39-46.
[13] 田万年,张子健,迟雪.吉林省部分地区蜱和羊携带羊泰勒虫和嗜吞噬细胞无浆体分子流行病学调查[J].中国兽医杂志,2023,59(6):9-15. TIAN W N,ZHANG Z J,CHI X.Molecular epidemiological investigation on ovine Theileria and Anaplasma phgocytophilum infections in ticks and sheep in selected regions of Jilin province[J].Chinese Journal of Veterinary Medicine,2023,59(6):9-15.(in Chinese)
[14] 朱奕霏,于龙政,吴保义,等.羊泰勒虫PCR检测及进化分析[J].延边大学农学学报,2019,41(4):67-72. ZHU Y F,YU L Z,WU B Y,et al.PCR detection and phylogenetic analysis of Theileria ovis[J].Journal of Agricultural Science Yanbian University,2019,41(4):67-72.(in Chinese)
[15] 李佳,段真真,白正辉,等.阿克苏地区羊感染泰勒虫病流行病学调查及分类鉴定[J].黑龙江畜牧兽医,2016,21:165-167. LI J,DUAN Z Z,BAI Z H,et al.Epidemiological investigation and classification identification of ovine theileriosis in Aksu area [J]. Heilongjiang Animal Science and Veterinary Medicine,2016,21:165-167.(in Chinese)
[16] ALLSOPP B A,BAYLIS H A,ALLSOPP M T,et al.Discrimination between six species of Theileria using oligonuc eotide probes which detect small subunit ribosomal RNA sequences[J].Parasitology,1993,107(2):157-165.
[17] 杨菊凤,田慧丽,郭瑞萍,等.山东省媒介蜱类种类鉴定及羊泰勒虫病流行病学调查[J].中国兽医学报,2015,35(6):930-937. YANG J F,TIAN H L,GUO R P,et al.Identification of tick species and epidemiological survey of ovine theileria parasite infection in Shandong province[J].Chinese Journal of Veterinary Science,2015,35(6):930-937.(in Chinese)
[18] 朱玉涛.新疆部分地区羊体表革蜱种属的鉴定、系统发育分析及其携带羊泰勒虫的分子诊断[D].乌鲁木齐:新疆农业大学,2017. ZHU Y T.Genus identification and phylogenetic analysis of Dermacentor on body surface and molecular diagnosis of theileriosis of Xinjiang sheep[D].Urumqi:Xinjiang Agricultural University,2017.(in Chinese)
[19] 宁东坡,张杨,肖培培,等.新疆博州部分地方羊泰勒虫病流行病学调查[J].云南畜牧兽医,2020,4:26-29. NING D P,ZHANG Y,XIAO P P,et al.Epidemiological survey of ovine theileriosis in parts of Bozhou,Xinjiang[J]. Yunnan Journal of Animal Science and Veterinary Medicine,2020,4:26-29.(in Chinese)
[20] 孟强飞,赵爱云,严勇,等.策勒县不同季节和田羊和策勒黑羊梨形虫感染情况调查[J].黑龙江畜牧兽医,2019,6:83-85. MENG Q F,ZHAO A Y,YAN Y,et al.Investigation on the infection of piroplasmosis in Hetian sheep and Cele black sheep in different seasons in Cele county[J].Heilongjiang Animal Science and Veterinary Medicine,2019,6:83-85.(in Chinese)
[21] 宋军科,彭先启,王雪婷,等.羊吕氏泰勒虫陕西分离株的鉴定及遗传进化分析[J].中国兽医学报,2018,38(2):307-310. SONG J K,PENG X Q,WANG X T,et al.Identification and phylogenetic analysis of Theileria luwenshuni from infected goats in Shaanxi province[J].Chinese Journal of Veterinary Medicine,2018,38(2):307-310.(in Chinese)
[22] KUNDAVE V R,NEHRA A K,RAM H,et al.Genetic diversity in the Tams1 gene of Theileria annulata (Duschunkowsky and Luhs,1904) infecting cattle[J].Acta Tropica,2021,224:106121.
[23] KATZER F,MCKELLAR S,KIRVAR E,et al.Phylogenetic analysis of Theileria and Babesia equi in relation to the establishment of parasite populations within novel host species and the development of diagnostic tests[J].Molecular and Biochemical Parasitology, 1998,95(1):33-44.

PCR Detection and Genetic Diversity Analysis of Theileria of Sheep in Aksu,Xinjiang

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