不同生长阶段滩羊背最长肌转录组比较

doi:10.16431/j.cnki.1671-7236.2025.03.004

Abstract

Abstract: 【Objective】 The purpose of this study was to explore the differential genes affecting fat deposition in longissimus dorsi muscle of Tan sheep at different growth stages.【Method】 The longissimus dorsi muscle of Tan sheep aged 3,6 and 9 months was collected for RNA extraction and library construction.The data was filtered,quality controlled,and compared using Illumian software.After HTSeq statistics,P_adj (corrected P-value)＜0.05 and |log₂FoldChange|＞1 were used as criteria to screen different expressed genes, GO function and KEGG pathway enrichment analysis was performed for genes, and Real-time quantitative PCR was performed to verify the 5 randomly selected genes.【Result】 Compared with 3 months of age,129 differentially expressed genes (47 up-regulated and 82 down-regulated) were detected at 6 months of age and 521 differentially expressed genes (119 up-regulated and 402 down-regulated) were detected at 9 months of age.Compared with 6 months of age,207 differentially expressed genes (45 up-regulated and 162 down-regulated) were detected at 9 months of age.GO function enrichment results showed that,compared with 3 months of age,the differentially expressed genes were enriched to arachidonic acid monooxygenase activity and phosphoglycerate dehydrogenase activity at 6 months of age and enriched to the positive regulation of cytoplasmic calcium ions,multicellular biological development regulation and other processes at 9 months of age.Compared with 6 months old,9 months old differentially expressed genes were enriched to intracellular calcium-mediated signal transduction and lipid phosphatase activity.The annotation results of KEGG pathway showed that compared with 3 months old,the differentially expressed genes at 6 months of age were mainly concentrated in the signaling pathways of cholesterol metabolism,amino sugar and nucleotide sugar metabolism and the differentially expressed genes at 9 months of age were mainly concentrated in signaling pathways such as extracellular matrix receptor interaction and insulin resistance.Compared with 6 months of age,the differentially expressed genes at 9 months of age were mainly concentrated in c-AMP signaling pathway,synthesis,secretion and action of growth hormone.Real-time fluorescence quantitative PCR results of 5 randomly selected genes,including FGF10 and ADCY7,were consistent with transcriptome sequencing results.【Conclusion】 Through transcriptomic sequencing analysis of longissimus dorsi muscle of Tan sheep at different ages,FGF10 and STAR were selected as candidate genes for the regulation of fat deposition in longissimus dorsi muscle of Tan sheep at 3 and 6 months of age,and ADCY7 and CNR1 were selected as candidate genes for the regulation of fat deposition in longissimus dorsi muscle of Tan sheep at 6 and 9 months of age.PPARGC1A and SLC2A4 were selected as candidate genes for the regulation of fat deposition in longissimus dorsi muscle of Tan sheep aged 3 and 9 months.The results provided theoretical basis for further investigation of fat deposition in longissimus dorsi muscle of Tan sheep at different growth stages.

Key words: Tan sheep; transcriptome; fat; longissimus dorsi muscle

CLC Number:

S826

DU Jiawen, REN Wenyi, XU Xiaofeng, ZHANG Lili. Comparison of the Transcriptome of Longissimus Dorsi Muscle of Tan Sheep at Different Growth Stages[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1011-1021.

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Comparison of the Transcriptome of Longissimus Dorsi Muscle of Tan Sheep at Different Growth Stages

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