不同嫩度徐州牛肌肉转录组比较分析

doi:10.16431/j.cnki.1671-7236.2025.02.003

Abstract

Abstract: 【Objective】 The gene expression differences of longissimus dorsi muscles with different tenderness in Xuzhou cattle were explored to identify the relevant regulatory genes and signaling pathways,providing a theoretical basis for further research on the mechanisms of muscle growth regulation in Xuzhou cattle. 【Method】 This study focused on a distinctive local cattle breed from Jiangsu,known as Xuzhou cattle.After slaughter,the longissimus dorsi muscle was assessed for shear force,dividing the samples into a high-tenderness group (n=6,shear force ＜5.5 kg) and a low-tenderness group (n=4,shear force＞7 kg).Transcriptome sequencing was conducted on muscle tissues from both groups.After filtering,quality control,alignment,and quantification of the sequencing data,differentially expressed genes were identified using the DESeq2 package in R language.Functional enrichment analysis was subsequently performed.Lastly,significantly differentially expressed genes,particularly cystatin B (CSTB),were analyzed for species homology,protein physicochemical properties,and other functional characteristics. 【Result】 ① Transcriptome analysis revealed that compared with the low-tenderness group,94 genes were up-regulated and 37 genes were down-regulated in the high-tenderness group.The top 20 differentially expressed genes included CSTB,VWF,DIRAS3,CDKN1A, CFB, etc. ② GO and KEGG enrichment analysis indicated that these differentially expressed genes were primarily involved in biological processes such as coagulation,immune response,wound healing,and the Wnt signaling pathway related to lipid metabolism.Gene pathway network analysis highlighted that CSTB,PRKG1,and PLAUR were among the differentially expressed genes shared across biological processes and molecular functions.③ The homology analysis of CSTB gene of cattle showed the highest similarity with Ovis aries at 97.96%,followed by Sus scrofa.The protein physicochemical properties analysis indicated that CSTB consists of 98 amino acid residues,with an instability index of 33.17 (＜40),suggesting that it was a stable protein.The prediction of protein secondary structure showed that CSTB was mainly composed of extended chain and random coil.STRING database analysis suggested potential interactions between CSTB and CTSH,WC1-10,and CTSB. 【Conclusion】 In this study,differentially expressed genes influencing the varying tenderness of Xuzhou cattle muscle were identified,including CSTB,VWF,DIRAS3,CDKN1A,and CFB.The results provide a theoretical basis and data support for beef quality research.

Key words: Xuzhou cattle; longissimus dorsi muscle; RNA-Seq; muscle tenderness

CLC Number:

S823.8⁺1

SHEN Yangyang, AN Zhenjiang, GAO Linna, XIA Shuwen, CHEN Kunlin, DING Qiang, GAO Yundong, XUE Jinguo, ZHONG Jifeng, WANG Huili. Comparative Analysis of Muscle Transcriptome of Xuzhou Cattle with Different Tenderness[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(2): 522-533.

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Comparative Analysis of Muscle Transcriptome of Xuzhou Cattle with Different Tenderness

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