牛骨骼肌卫星细胞发育过程中的转录组和翻译组联合分析

doi:10.16431/j.cnki.1671-7236.2024.11.002

Abstract

Abstract: 【Objective】 The aim of this study was to analyze and compare the growth and development process of bovine skeletal muscle at two different levels of transcription and translation using transcriptome sequencing (RNA-Seq) and ribosome-nascent-chain-complex sequencing (RNC-Seq), analyze the developmental characteristics and patterns,and screen out key genes that might be involved in the growth and development process of bovine muscle in the transcriptome and translatome,in order to provide a target for the subsequent screening and utilization of functional genes. 【Method】 Samples of the proliferative period (GM) and the second day of differentiation (DM2) of skeletal muscle satellite cells from 4-month-old bovine embryos were collected for RNA-Seq and RNC-Seq analysis.The differentially expressed genes were analyzed using bioinformatics,and the differentially expressed genes related to skeletal muscle development were analyzed from the perspective of translational regulation. 【Result】 The differential gene screening of BSMSCs in DM2 phase compared to GM phase showed that a total of 2 808 differential genes (1 749 up-regulated genes and 1 059 down-regulated genes) were obtained from transcriptome screening,and 3 740 differential genes (1 769 up-regulated genes and 1 971 down-regulated genes) were obtained from translatome screening.There were 725 differentially expressed genes shared between transcriptome and translatome,including 618 genes with similar expression trends and 107 genes with opposite expression trends.The functional enrichment analysis results showed that 103 genes related to skeletal muscle development were obtained.Further in-depth analysis showed that the transcriptional and translational regulation were relatively independent and the translational regulation had a certain effect of retrogression on the transcriptional regulation,22 target genes closely related to the growth and development of bovine muscle (CDK1,SERPINE1,THBS1,IGFBP3,CNB1,GADD45A,CYCS,CDK2,CCND1,CCNE2,ZMAT3,ACTB,ACTA1,TNNT1,MYH7,ACTG1,TUBA1C,TNNC1,KIF20A,TUBB4B,MYL3 and TUBB3) and 13 key genes with translation retrogression (GREM1,EGR1,SFRP4,NKD2,DKK3,IGFBP3,CTSK,ITGAV,CTSV,CDKN1A,SESN3,GADD45A and ZMAT3) were obtained. 【Conclusion】 In this study,22 key genes that might be closely related to the growth and development of bovine muscles and 13 key genes with translation callbacks were screened,providing important targets for further regulating the process of bovine muscle growth and development through molecular means for new breed cultivation.

Key words: bovine skeletal muscle satellite cells; RNA-Seq; RNC-Seq; bioinformatics

CLC Number:

S813

MA Sufang, YANG Wenqing, ZHANG Bingbing, GUO Ruonan, XU Yingxin, ZHANG Linlin, GUO Yiwen, HU Debao, GUO Hong, DING Xiangbin, LI Xin. Joint Analysis of Transcriptome and Translatome During the Development of Bovine Skeletal Muscle Satellite Cells[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(11): 4665-4677.

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Joint Analysis of Transcriptome and Translatome During the Development of Bovine Skeletal Muscle Satellite Cells

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