合作猪SIRT3基因克隆、生物信息学分析及组织表达研究

doi:10.16431/j.cnki.1671-7236.2024.04.007

Abstract

Abstract: 【Objective】 The CDS region of silence information regulator 3 (SIRT3) gene in Hezuo pigs was cloned and bioinformatic analysis was performed,and the expression level in different tissues of Hezuo pigs was explored,laying a foundation for the study of the function of SIRT3 gene.【Method】 Three 3-month-old Hezuo boars were selected to collect heart,testis,lung and other tissues.The CDS region sequence of SIRT3 gene was obtained by PCR amplification and Sanger sequencing,and the phylogenetic tree was constructed by Mega 7.0.Bioinformatic analysis of SIRT3 protein was performed by online software.The tissue expression of SIRT3 gene was detected by Real-time quantitative PCR.【Result】 The CDS region of SIRT3 gene was 774 bp,encoding 257 amino acids.Hezuo pig had the closest relationship with domestic pig,and had a distant relationship with zebrafish.The molecular weight of SIRT3 protein was 28.68 ku,the theoretical isoelectric point was 5.81,and the instability coefficient was 37.92,indicating that SIRT3 protein was a stable hydrophilic protein.SIRT3 protein had no transmembrane region and no signal peptide,but there were 2 low complexity region,which were mainly distributed in the endoplasmic reticulum.The secondary structure of SIRT3 protein was mainly alpha-helix and random coil,and the results predicted by the tertiary structure model were basically consistent with the secondary structure.Real-time quantitative PCR results showed that SIRT3 gene was expressed in different tissues of Hezuo pigs,and the expression level in heart was the highest,which was extremely significantly higher than that in other tissues (P＜0.01),followed by testis,lung and liver tissues.【Conclusion】 The CDS region sequence of SIRT3 gene in Hezuo pigs was successfully cloned,the expression level of SIRT3 gene in different tissues of Hezuo pigs was explored,and the biological function of its encoded protein was preliminatively analyzed,which provided a reference for further study of SIRT3 gene function in Hezuo pigs.

Key words: Hezuo pigs; SIRT3 gene; cloning; bioinformatics analysis; tissue expression

CLC Number:

S828.8⁺9

YAN Zunqiang, LIANG Yuhao, SONG Kelin, GUN Shuangbao, WANG Pengfei. Cloning,Bioinformatics Analysis and Tissue Expression of SIRT3 Gene in Hezuo Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1390-1399.

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Cloning,Bioinformatics Analysis and Tissue Expression of SIRT3 Gene in Hezuo Pigs

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