贵州白山羊肌细胞生成素基因克隆与生物信息学分析

doi:10.16431/j.cnki.1671-7236.2024.03.004

Abstract

Abstract: 【Objective】 This study was aimed to clone myogenin gene in Guizhou White goat and analyze the structure characteristics by bioinformatics,so as to provide an important reference basis for further exploring molecular regulation mechanism of muscle development of goat.【Method】 The blood genomic DNA of Guizhou White goat was used as template,four pairs of specific primers were designed,and the complete coding sequence of myogenin gene in Guizhou White goat was obtained by PCR amplification,Sanger sequencing and sequence assembly method.The molecular structure characteristics of myogenin gene,such as composition of nucleotide and amino acid sequence,phosphorylation sites,key domains and functional motifs were analyzed by BioEdit 7.0,NetPhos 3.1,Conserved Domains Search,SMART and Motif Scan softwares,respectively.The sequence similarity comparison of myogenin gene among 15 Artiodactyla species and phylogenetic tree construction were carried out by DNAStar Lasergene,DNAMAN 8.0 and Mega 5.0 softwares.【Result】 The length of myogenin gene sequence in Guizhou White goat was 2 424 bp(obtain GenBank accession No.:HZ53289),which was consisted of 3 exons,2 introns and partial of 5'-UTR and 3'-UTR,and was 471,82,122,765,589,135 and 260 bp in length,respectively.The length of coding region was 675 bp,which was encoded 224 amino acids.The deduced amino acid sequence analysis showed that 1-86 amino acids were the unique basic domain of myogenic determining factors family,81-139 amino acids were the helix-loop-helix DNA binding domain,and 160-170 amino acids were the low complexity regions.There were 21 phosphorylation sites and functional motif structure such as nuclear localization signal protein.Amino acid sequence alignment showed that six cysteine residues,one nuclear localization signal protein and one low complexity region were completely conserved among 15 Artiodactyla species.Sequence similarity and molecular phylogenetic analysis all showed that Guizhou White goat myogenin gene had the closest genetic relationship with Boer goat,Hu sheep and Marco Polo sheep.【Conclusion】 The myogenin gene of Guizhou White goat was successfully cloned,with a size of 2 424 bp in length and encoding 224 amino acids,which contained the Basic and H-L-H domains.These results could provide theoretical reference for further exploring the molecular regulation mechanism of muscle growth and development of goat.

Key words: Guizhou White goat; myogenin gene; cloning; molecular phylogenetic evolution; bioinformatics

CLC Number:

S827
S813.3

SONG Xingchao, MENG Jinzhu, ZHAO Yuanyuan, WU Zhenyang, AN Qingming. Cloning and Bioinformatics Analysis of myogenin Gene in Guizhou White Goat[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 926-935.

References

[1] WEI D, ZHANG J, RAZA S H A, et al.Interaction of MyoD and MyoG with Myoz2 gene in bovine myoblast differentiation[J].Research in Veterinary Science, 2022, 152(20):569-578.
[2] HERNÁNDEZ-HERNÁNDEZ J M, GARCÍA-GONZÁLEZ E G, BRUN C E, et al.The myogenic regulatory factors, determinants of muscle development, cell identity and regeneration[J].Seminars in Cell&Developmental Biology, 2017, 72(12):10-18.
[3] OLIANI L C, LIDANI K, GABRIEL J E.Differentiated evolutionary relationships among chordates from comparative alignments of multiple sequences of MyoD and MyoG myogenic regulatory factors[J].Genetics and Molecular Research, 2015, 14(4):12561-12566.
[4] SOUSA-JUNIOR L P B, MEIRA A N, AZEVEDO H C, et al.Variants in myostatin and MyoD family genes are associated with meat quality traits in Santa Inês sheep[J].Animal Biotechnology, 2022, 33(2):201-213.
[5] 李静云,王新乐,张云飞,等.绵羊MyoG基因内含子Ⅱ多态性及其与生长性状的关联分析[J].中国畜牧兽医, 2022, 49(4):1364-1373. LI J Y, WANG X L, ZHANG Y F, et al.Polymorphism of MyoG gene intron Ⅱ and its association with growth traits in sheep[J].China Animal Husbandry&Veterinary Medicine, 2022, 49(4):1364-1373.(in Chinese)
[6] 刘铮铸,巩元芳,付志新,等.山羊MyoG基因内含子Ⅱ变异及其在体质量上的遗传效应分析[J].畜牧兽医学报, 2011, 42(7):1015-1021. LIU Z Z, GONG Y F, FU Z X, et al.Genetic variation of goat MyoG gene intron Ⅱ and its genetic effects on body weight[J].Acta Veterinaria et Zootechnica Sinica, 2011, 42(7):1015-1021.(in Chinese)
[7] 许厚强.贵州地方畜禽遗传资源志[M].北京:中国农业出版社, 2019. XU H Q.Animal Genetic Resources in Guizhou[M].Beijing:China Agriculture Press, 2019.(in Chinese)
[8] 国家畜禽遗传资源委员会.中国畜禽遗传资源志--羊志[M].北京:中国农业出版社, 2012. CHINA NATIONAL COMMISSION OF ANIMAL GENETIC RESOURCES.Animal Genetic Resources in China Sheep and Goats[M].Beijing:China Agriculture Press, 2012.(in Chinese)
[9] LI L, YANG P, SHI S, et al.Association analysis to copy number variation (CNV) of Opn4 gene with growth traits of goats[J].Animals(Basel), 2020, 10(3):441-448.
[10] 刘铮铸,巩元芳,张传生,等.波尔山羊MyoG基因的鉴定和序列分析[J].畜牧兽医学报, 2010, 41(10):1337-1341. LIU Z Z, GONG Y F, ZHANG C S, et al.Identification and sequence analysi of MyoG gene in Boer goat (Capra hircus)[J].Acta Veterinaria et Zootechnica Sinica, 2010, 41(10):1337-1341.(in Chinese)
[11] 赵子贵,黄晨,宋少锐,等.隆林山羊肌细胞生成素基因的序列分析与结构预测[J].中国畜牧兽医, 2012, 39(11):17-21. ZHAO Z G, HUANG C, SONG S R, et al.Sequence analysis and structure prediction of MyoG gene in Longlin goat[J].China Animal Husbandry&Veterinary Medicine, 2012, 39(11):17-21.(in Chinese)
[12] 宋兴超,孟金柱,赵园园,等.贵州白山羊MyoG基因启动子区序列克隆及生物信息学分析[J].中国畜牧兽医, 2023, 50(3):893-903. SONG X C, MENG J Z, ZHAO Y Y, et al.Cloning and bioinformatics analysis of MyoG gene promoter region sequence in Guizhou White goat[J].China Animal Husbandry&Veterinary Medicine, 2023, 50(3):893-903.(in Chinese)
[13] KOZAK M.Structural features in eukaryotic mRNAs that modulate the initiation of translation[J].Journal of Biological Chemistry, 1991, 266(30):19867-19870.
[14] 王宁,陈润生.基于内含子和外显子的系统发育分析的比较[J].科学通报, 1999, 44(19):2095-2102. WANG N, CHEN R S.Comparison of phylogenetic analysis based on intron and exon[J].Chinese Science Bulletin, 1999, 44(19):2095-2102.(in Chinese)
[15] 宋兴超,刘汇涛,岳志刚,等.甘肃马鹿myogenin基因克隆、序列结构特征预测及其分子系统进化分析[J].四川动物, 2013, 32(1):51-59. SONG X C, LIU H T, YUE Z G, et al.Cloning, bioinformatics prediction on structural characteristics and phylogenetic evolution analysis of myogenin gene in Cervus elaphus kansuensis[J].Sichuan Journal of Zoology, 2013, 32(1):51-59.(in Chinese)
[16] 宋兴超,徐超,刘琳玲,等.吉林梅花鹿肌肉发育关联基因MyoG的鉴定及生物信息学分析[J].特产研究, 2019, 41(4):11-16. SONG X C, XU C, LIU L L, et al.Identification and bioinformatics analysis of MyoG gene related to muscle development in Jilin sika deer (Cervus nippon)[J].Special Wild Economic Animal and Plant Research, 2019, 41(4):11-16.(in Chinese)
[17] SOUMILLION A, ERKENS J H F, LENSTRA J A, et al.Genetic variation in the porcine myogenin gene locus[J].Mammalian Genome, 1997, 8(8):564-568.
[18] 郭金虎,单祥年,常青,等.赤麂SRY基因的测序及其与部分偶蹄目动物SRY基因序列的比较[J].动物学报, 2001, 47(2):145-149. GUO J H, SHAN X N, CHANG Q, et al.Sequencing SRY gene of Muntiacus muntiak vaginalis and a phylogenetic comparison with some of Artiodactyla[J].Acta Zoologica Sinica, 2001, 47(2):145-149.(in Chinese)
[19] 侯佩兴,孙立彬,赵洪波.偶蹄目牛科和鹿科SRY基因编码区进化研究[J].上海交通大学学报(农业科学版), 2009, 27(4):350-352. HOU P X, SUN L B, ZHAO H B.Evolution of SRY gene encoding region in the Bovidae and Gervidae of the Artiodactyla[J].Journal of Shanghai Jiaotong University(Agricultural Science), 2009, 27(4):350-352.(in Chinese)
[20] 何学谦,陈昌维.会理黑山羊MyoG基因的鉴定及序列分析[J].畜牧与兽医, 2014, 46(4):46-49. HE X Q, CHEN C W.Identification and sequence analysis of MyoG gene in Huili Black goat[J].Animal Husbandry&Veterinary Medicine, 2014, 46(4):46-49.(in Chinese)
[21] TE PAS M F, SOUMILLION A, HARDERS F L, et al.Influences of myogenin genotypes on birth weight, growth rate, carcass weight, backfat thickness, and lean weight of pigs[J].Journal of Animal Science, 1999, 77(9):2352-2356.
[22] GENXI Z, YING T, TAO Z, et al.Expression profiles and association analysis with growth traits of the MyoG and Myf5 genes in the Jinghai Yellow chicken[J]. Molecular Biology Reports, 2014, 41(11):7331-7338.
[23] ZHANG Z, XU F, ZHANG Y, et al.Cloning and expression of MyoG gene from Hu sheep and identification of its myogenic specificity[J].Molecular Biology Reports, 2014, 41(2):1003-1013.
[24] MEADOWS E, CHO J H, FLYNN J M, et al.Myogenin regulates a distinct genetic program in adult muscle stem cells[J].Developmenatal Biology, 2008, 322(2):406-414.

Cloning and Bioinformatics Analysis of myogenin Gene in Guizhou White Goat

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