不同嫩度牦牛背最长肌中SERPINE1基因克隆、生物信息学及表达分析

doi:10.16431/j.cnki.1671-7236.2024.06.006

Abstract

Abstract: 【Objective】 This study was aimed to clone the serpin family E member 1 (SERPINE1) gene sequence in yak,and explore the nucleotide sequence difference of SERPINE1 gene in longissimus dorsi muscle of yak with different tenderness,so as to provide experimental data for further study on its effect on the tenderness of yak meat.【Method】 Based on the yak SERPINE1 gene sequence in GenBank,specific primers were designed to amplify the full length of SERPINE1 gene sequence in longissimus dorsi muscle of yak with high and low tenderness by PCR,and their structure and encoded protein were analyzed by bioinformatics.Real-time quantitative PCR was used to detect SERPINE1 gene expression in longissimus dorsi muscle of yak with different tenderness.【Result】 The total length of SERPINE1 gene in longissimus dorsi muscle of yak with high and low tenderness was 8 315 and 8 318 bp,respectively,encoding 402 amino acids with identical amino acid sequence.There were 3 base deletions and 22 base mutations (4 base translocations and 18 base transitions) of SERPINE1 gene in high and low tenderness longissimus dorsi muscle.The nucleotide sequence of SERPINE1 gene in yak showed 99.26%,99.26%,99.59%,96.94%,97.02%,90.49%,86.52% and 80.10% similarity to Bos taurus,Bos indicus,Bison bison bison,Ovis aries,Capra hircus,Sus scrofa,Homo sapicns and Mus musculus,respectively.SERPINE1 protein was a hydrophilic stable extracellular protein containing 23 amino acid signaling peptides, and located in the extracellular with 34 potential phosphorylation sites,including 1 reactive center loop (RCL).The secondary structure of SERPINE1 protein was mainly composed of alpha helix (44.78%) and random coil (35.32%).Real-time quantitative PCR results showed that the expression of SERPINE1 gene in longissimus dorsi muscle of yak with high tenderness was extremely significantly higher than that in longissimus dorsi muscle of yak with low tenderness (P＜0.01).【Conclusion】 The full length of SERPINE1 gene was successfully cloned from longissimus dorsi muscle of yak with high and low tenderness and the bioinformatics characteristics were analyzed,which provided theoretical reference for the subsequent research on the mechanism of SERPINE1 gene involved in the regulation of yak meat tenderness.

Key words: yaks; SERPINE1 gene; tenderness; bioinformatics; expression

CLC Number:

S823.8⁺5

JING Kemin, ZHANG Peng, LI Yuqian, TIAN Yuan, DONG Wenjing, CHAI Zhixin, WANG Jikun, ZHONG Jincheng, CAI Xin. Cloning, Bioinformatic and Expression Analysis of SERPINE1 Gene in Longissimus Dorsi Muscle of Yaks with Different Tenderness[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2308-2318.

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Cloning, Bioinformatic and Expression Analysis of SERPINE1 Gene in Longissimus Dorsi Muscle of Yaks with Different Tenderness

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