高精料饲粮中添加辣蓼对绵羊瘤胃发酵参数和瘤胃菌群的影响

doi:10.16431/j.cnki.1671-7236.2025.01.018

Abstract

Abstract: 【Objective】 The experiment aimed to investigate the effects of adding Polygonum hydropiper L.to high-concentrate diet on rumen fermentation parameters,microbial populations,lactic acid metabolism,and enzyme activity in sheep. 【Method】 Forty 3-month-old Duhan hybrid male sheeps with an average weight of (23.26±0.33) kg were selected and randomly divided into four groups with 10 sheeps per group.Lambs were fed individually with a crude-to-processed diet ratio of 75∶25.The levels of Polygonum hydropiper L.supplementation were 0 (control),5 (L1),10 (L2),and 15 g/d (L3).After a 15-day adaptation period,a 60-day experimental period was conducted,lasting 75 days.Rumen fluid samples were collected from all lambs 3 hours after morning feeding at the end of the experimental period for subsequent analysis of rumen fermentation parameters,microbial enzyme activities,lactic acid metabolism,and microbial populations. 【Result】 ① The total volatile fatty acid (TVFA) content in rumen fluid of sheep in group L3 was significantly higher than that in control group and L1 group (P＜0.05),the propionic acid content was significantly higher than that in the other three groups (P＜0.05),the ratio of ethylene to propylene was significantly lower than that in control group,and the ammonia nitrogen (NH₃-N) content was significantly lower than that in L1 group and control group (P＜0.05).The content of microbial protein (MCP) was significantly higher than that in L1 group and control group (P＜0.05).The contents of propionic acid and MCP in rumen fluid of sheep in L2 group were significantly higher than those in control group (P＜0.05),the ratio of ethylene to propylene and the content of NH₃-N in L2 group were significantly lower than those in control group (P＜0.05),the content of NH₃-N in rumen fluid of sheep in L1 group was significantly lower than that in control group (P＜0.05).② The activity of cellobiase in rumen fluid of sheep in L2 and L3 groups was significantly higher than that in control group (P＜0.05),and the activity of protease was significantly lower than that in control group (P＜0.05),the protease activity in rumen fluid of sheep in L3 group was also significantly lower than that in L1 group (P＜0.05),and the α-amylase activity was significantly higher than that in L1 group and control group (P＜0.05).③ Lactic acid content in rumen fluid of sheep in L3 group was significantly lower than that in control group and L1 group (P＜0.05),and lactate dehydrogenase activity was significantly lower than that in other three groups (P＜0.05).Pyruvate content and pyruvate kinase activity in rumen fluid of sheep in 3 experimental groups were significantly higher than those of control group,and L3 group was significantly higher than L2 and L1 groups (P＜0.05).④ The number of total methanogens in rumen fluid of sheep in group L3 was significantly lower than that in control group (P＜0.05),the number of total protozoa and Prevotella ruminalis was significantly lower than that in L1 group and control group (P＜0.05),and the number of Ruminococcus albus was significantly higher than that in control group (P＜0.05).The number of Ruminbacter amylophilus was significantly higher than that in L1 and control groups (P＜0.05).The number of total protozoa and Prevotella ruminalis in rumen fluid of sheep in L2 group was significantly lower than that in control group (P＜0.05),and the number of Ruminbacter amylophilus was significantly higher than that in control group (P＜0.05). 【Conclusion】 The addition of Polygonum hydropiper L. to a high-concentrate diet influenced rumen microbiota,improved fermentation,reduced A/P ratio,enhanced pyruvate-related protein synthesis,decreased NH₃-N concentrations,and regulated lactic acid metabolism by altering enzyme activities,thereby reducing lactic acid accumulation.Under these conditions,the optimal supplementation level was determined to be 15 g/d.

Key words: high-concentrate diet; Polygonum hydropiper L.; sheep; rumen fermentation; lactic acid

CLC Number:

S826.92

YU Tianli, HE Zhenlian, HAN Yijing, REN Jingyu, XIA Chengqiang, PEI Caixia. Effects of Adding Polygonum hydropiper L.to High-concentrate Diet on Fermentation Parameters and Flora of Rumen in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 205-214.

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Effects of Adding Polygonum hydropiper L.to High-concentrate Diet on Fermentation Parameters and Flora of Rumen in Sheep

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