不同因素对特哈杂交绵羊消化道线虫感染情况的影响

doi:10.16431/j.cnki.1671-7236.2024.07.031

Abstract

Abstract: 【Objective】 This experiment was aimed to study the effects of season,gender,group and flock on the infection intensity of gastrointestinal nematodes (GIN),weight,hematocrit and specific antibody immunoglobulin G (IgG) content in Texel and Kazakh (hereinafter referred to as Teha) crossbred sheep,in order to provide a reference basis for research on the prevention and treatment of GIN in sheep and anti-GIN breeding.【Method】 Feces were collected rectally,blood was collected from the jugular vein,and weights were weighed in Teha crossbred sheep.The eggs of GIN were qualitatively detected by saturated brine flotation method.The modified McMaster method was used for egg counting of GIN,Infection rate and infection intensity were calculated.Hematocrit was measured using a veterinary auto hematology analyzer.Nematode L3 larval somatic cell antigens were prepared,and specific antibody IgG content in the serum of the sheep was determined using indirect ELISA method.【Result】 The infection rate of GIN in Teha crossbred sheep was high at 94.44%,which was moderate. And total of nine GIN were identified which were Trichostrongylus spp.,Haemonchus contortus,Ostertagia spp.,Bumastomum trigonocephalum,Chabertia spp.,Marshallagia spp.,Nematodirus spp.,Oesophagostomum spp.and Trichuris globuulosa.Season had a significant effect on the infection intensity of GIN and weight of sheep (P＜0.05),the infection intensity of GIN was significantly higher in spring than that in summer (P＜0.05),and the weight of sheep was significantly higher in summer than that in spring (P＜0.05).Gender had a significantly effect on the infection intensity,weight and hematocrit (P＜0.05),with the infection intensity of GIN significantly higher in rams than in ewes (P＜0.05),the weight was significantly higher in rams than in ewes (P＜0.05),and hematocrit was significantly higher in ewes than in rams (P＜0.05).Group had a significant effect on weight (P＜0.05),the weight of F2×Ha sheep was significantly higher than that of F2 and F2×F1 sheep (P＜0.05),the weight of F1×F1 sheep was significantly higher than that of F2×F1 sheep (P＜0.05),and the weight of F2×F2 sheep was significantly lower than that of other groups (P＜0.05).Flock had a significant effect on the infection intensity in sheep (P＜0.05),with the infection intensity of GIN in flock 1 being significantly higher than that in flocks 2 and 3 (P＜0.05),and the infection intensity of GIN in flock 3 was significantly higher than that in flock 2 (P＜0.05).All factors had no effect on specific antibody IgG content (P＞0.05).【Conclusion】 Season,gender,group and flock had influence on the infection of GIN in sheep,and different control measures could be selected for sheep through the effect of factors on the infection and the differences between different levels of each factor,to provide a theoretical basis for anti-GIN breeding strategies.

Key words: Teha crossbred sheep; gastrointestinal nematodes; infection rate; intensity of infection

CLC Number:

S852.73⁺1

WANG Yuqi, HE Sangang, LIANG Long, HAILATI Kasimu, JIAO Wenjing, YUE Chenbing, HAN Bing, SONG Xuebin, YAN Xiaofei. Effect of Different Factors on the Infection of Gastrointestinal Nematodes in Teha Crossbred Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 3067-3076.

References

[1] ROEBER F,JEX A R,GASSER R B.Impact of gastrointestinal parasitic nematodes of sheep,and the role of advanced molecular tools for exploring epidemiology and drug resistance-An Australian perspective[J].Parasites&Vectors,2013,6(1):153.
[2] BISSET S A,VLASSOFF A,DOUCH P G,et al.Nematode burdens and immunological responses following natural challenge in Romney lambs selectively bred for low or high faecal worm egg count[J].Veterinary Parasitology,1996,61(3-4):249-263.
[3] GRAY G D.The use of genetically resistant sheep to control nematode parasitism[J].Veterinary Parasitology,1997,72(3-4):345-366.
[4] ZVINOROVA P I,HALIMANI T E,MUCHADEYI F C,et al.Breeding for resistance to gastrointestinal nematodes-The potential in low-input/output small ruminant production systems[J].Veterinary Parasitology,2016,225:19-28.
[5] MAVROT F,HERTZBERG H,TORGERSON P.Effect of gastrointestinal nematode infection on sheep performance:A systematic review and Meta-analysis[J].Parasites&Vectors,2015,8(1):557.
[6] ARZIK Y,KIZILASLAN M,WHITE S N,et al.Genomic analysis of gastrointestinal parasite resistance in Akkaraman sheep[J].Genes(Basel),2022,13(12):2177.
[7] HECKENDORN F,BIEBER A,WERNE S,et al.The genetic basis for the selection of dairy goats with enhanced resistance to gastrointestinal nematodes[J].Parasite(Paris,France),2017,24:32.
[8] LACROUX C,NGUYEN T H,ANDREOLETTI O,et al.Haemonchus contortus(Nematoda:Trichostrongylidae) infection in lambs elicits an unequivocal Th2 immune response[J].Veterinary Research,2006,37(4):607-622.
[9] 钱德兴,龙鳌,曾红,等.铜仁、遵义、毕节三地(市)九县牛羊寄生蠕虫消长规律调查研究[J].中国兽医寄生虫病,2000,8(4):28-30. QIAN D X,LONG A,ZENG H,et al.Investigation and research on the pattern of growth and decline of parasitic helminths in cattle and sheep in nine counties of Tongren,Zunyi,and Bijie[J].Chinese Journal of Veterinary Parasitology,2000,8(4):28-30.(in Chinese)
[10] SISSAY M M,UGGLA A,WALLER P J.Epidemiology and seasonal dynamics of gastrointestinal nematode infections of sheep in a semi-arid region of Eastern Ethiopia[J].Veterinary Parasitology,2007,143(3-4):311-321.
[11] ZUK M,MCKEAN K A.Sex differences in parasite infections:Patterns and processes[J].International Journal for Parasitology,1996,26(10):1009-1023.
[12] 阎晓菲,刘明军.绵羊抗消化道线虫感染育种技术研究进展[J].福建农业学报,2020,35(8):911-918. YAN X F,LIU M J.Research progress on breeding technology of sheep resistance to gastrointestinal nematode infection[J].Fujian Journal of Agricultural Sciences,2020,35(8):911-918.(in Chinese)
[13] YAN X F,LIU M J,HE S G,et al.An epidemiological study of gastrointestinal nematode and Eimeria coccidia infections in different populations of Kazakh sheep[J].PLoS One,2021,16(5):e0251307.
[14] 孔繁瑶.家畜寄生虫学[M].2版.北京:中国农业大学出版社,2016. KONG F Y.Livestock Parasitology[M].2nd ed.Beijing:China Agricultural University Press,2016.(in Chinese)
[15] ZAJAC A M,CONBOY G A.Veterinary Clinical Parasitology[M].8th ed.Iowa,USA:Johnwiley and Sons,Inc.,2012.
[16] MAFF.Manual of Veterinary Parasitological Laboratory Techniques[M].London,UK:Ministry of Agriculture,Fisheries and Food.Technical Bulletin,1986.
[17] YAN X F,HE S G,LIU Y Y,et al.Molecular identification and phylogenetic analysis of gastrointestinal nematodes in different populations of Kazakh sheep[J].Experimental Parasitology,2023,254:108625.
[18] 原建琦.辐照致弱捻转血矛线虫L3期幼虫诱导羊保护性免疫效果及其机制的初步研究[D].上海:上海师范大学,2021. YUAN J Q.Preliminary study on the protective immune effect and mechanism of the L3 larvae of Haemonchus contortus induced by irradiation in sheep[D].Shanghai:Shanghai Normal University,2021.(in Chinese)
[19] GAULY M,KRAUS M,VERVELDE L,et al.Estimating genetic differences in natural resistance in Rhön and Merinoland sheep following experimental Haemonchus contortus infection[J].Veterinary Parasitology,2002,106(1):55-67.
[20] MANDAL A,YADAV N K,SHARMA D K,et al.Transformation methods for analysis of data on faecal egg count in sheep[J].Indian Journal of Animal Sciences,2008,78:364-366.
[21] 艾山江·塔斯坦,比尔来西肯·赛都力.伊犁河谷牧区哈萨克羊线虫病流行状况分析[J].畜禽业,2021,32(7):15-16. AISHANJIANG T,BIERLAIXIKEN S.Analysis of the epidemiological status of nematode disease in Kazakh sheep in pastoral areas of the Ili River Valley[J].Livestock and Poultry Industry,2021,32(7):15-16.(in Chinese)
[22] CHAGAS A C,VIEIRA L S,FREITAS A R,et al.Anthelmintic efficacy of neem (Azadirachta indica A.Juss) and the homeopathic product Fator Vermes in Morada Nova sheep[J].Veterinary Parasitology,2008,151(1):68-73.
[23] SINGH E,KAUR P,SINGLA L D,et al.Prevalence of gastrointestinal parasitism in small ruminants in Western zone of Punjab,India[J].Veterinary World,2017,10(1):61-66.
[24] 阎晓菲,刘宜勇,童婷,等.伊犁不同地区绵羊消化道线虫和球虫感染的季节动态调查[J].家畜生态学报,2022,43(8):66-72. YAN X F,LIU Y Y,TONG T,et al.Seasonal dynamics of sheep gastrointestinal nematodes and coccidia infection in different areas of Ili[J].Acta Ecologiae Animalis Domastici,2022,43(8):66-72.(in Chinese)
[25] 贺鑫,李佳莹,何鹏飞,等.特哈杂交羔羊消化道线虫的遗传参数估计及其与生长性状关系[J].草食家畜,2023,1:1-8. HE X,LI J Y,HE P F,et al.Estimation of genetic parameters of digestive tract nematodes in Teha cross lambs and its relationship with growth traits[J].Grass Feeding Livestock,2023,1:1-8.(in Chinese)
[26] IDRIS A,MOORS E,SOHNREY B,et al.Gastrointestinal nematode infections in German sheep[J].Parasitology Research,2012,110(4):1453-1459.
[27] GOLDING N,SMALL R W.The relative resistance to gastrointestinal nematode infection of three British sheep breeds[J].Research in Veterinary Science,2009,87(2):263-264.
[28] MILLER J E,BAHIRATHAN M,LEMARIE S L,et al.Epidemiology of gastrointestinal nematode parasitism in Suffolk and Gulf Coast Native sheep with special emphasis on relative susceptibility to Haemonchus contortus infection[J].Veterinary Parasitology,1998,74(1):55-74.
[29] AMARANTE A F,SUSIN I,ROCHA R A,et al.Resistance of Santa Ines and crossbred ewes to naturally acquired gastrointestinal nematode infections[J].Veterinary Parasitology,2009,165(3-4):273-280.
[30] HAYWARD A D,GARNIER R,WATT K A,et al.Heritable,heterogeneous,and costly resistance of sheep against nematodes and potential feedbacks to epidemiological dynamics[J].The American Naturalist,2014,184(S1):S58-S76.
[31] NUSSEY D H,WATT K A,CLARK A,et al.Multivariate immune defences and fitness in the wild:Complex but ecologically important associations among plasma antibodies,health and survival[J]. Proceedings Biological Sciences,2014,281(1779):20132931.
[32] AL-SHAIBANI I,PHULAN M,ARIJO A,et al.Contamination of infective larvae of gastrointestinal nematodes of sheep on communal pasture[J].Biology,2008,10(6):653-657.
[33] REEG K J,GAULY M,BAUER C,et al.Coccidial infections in housed lambs:Oocyst excretion,antibody levels and genetic influences on the infection[J].Veterinary Parasitology,2005,127(3-4):209-219.
[34] 赵乾明,齐萌,徐泽立,等.规模舍饲羊场羊消化道及环境寄生虫情况调查[J].中国畜牧兽医,2023,50(5):2156-2165. ZHAO Q M,QI M,XU Z L,et al.Investigation of intestinal parasites occurrence in sheep and environmental contamination in large scale housing farms[J].China Animal Husbandry&Veterinary Medicine,2023,50(5):2156-2165.(in Chinese)
[35] 景纪春,宁晓冬,崔艳艳,等.规模化全舍饲湖羊消化道寄生虫感染情况调查[J].中国畜牧兽医,2019,46(7):2103-2109. JING J C,NING X D,CUI Y Y,et al.Survey prevalence of gastrointestinal parasites in Hu sheep from stal-feeding farms[J].China Animal Husbandry&Veterinary Medicine,2019,46(7):2103-2109.(in Chinese)

Effect of Different Factors on the Infection of Gastrointestinal Nematodes in Teha Crossbred Sheep

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