云南边境地区流行性出血热病毒血清学监测及血清型鉴定

doi:10.16431/j.cnki.1671-7236.2019.10.029

Abstract

Abstract: To study the epidemic situation of epidemic hemorrhagic disease virus (EHDV) in cattle and goats from Jiangcheng county in Yunnan province in recent years,in this study,three monitoring points were set up for three consecutive years from 2014 to 2016.Ten cattle and five goats with negative EHDV antibodies were selected as sentinel animals every year.Blood was collected once a week from May to October,and once a month in November and December,which were used to monitor antibody and antigen,and were used to isolate virus.A group specific S7 gene fragment primer was used to detect the samples of cytopathic lesions by RT-PCR method.At the same time,EHDV-1,-5,-6,-7,-10 standard positive serums were used to neutralize and identify the isolated viruses.The results showed that the positive rates of EHDV antibody from 2014 to 2016 were 41.9%,58.6% and 75.4%,respectively;In three years,15 cattle of EHDV positive were found in Jiangcheng county,and twenty samples were isolated from them.The RT-PCR result confirmed the EHDV,and genetic evolution analysis found that 11 strains were closely related to the EHDV strains isolated in Japan in 1997 and 2003,9 strains were closely related to the separation of Australia in 1977 and 1981,and 5 strains were closely related to the separation of Guangxi in 2015;Neither antibody nor antigen-positive animal was detected in goats for 3 years;After the neutralization test serotype identification,20 strains were determined to include 4 serotypes of EHDV-5,-6,-7,and -10,and the infection time was between May and September.This study found that there were multiple serotypes of EHDV in Jiangcheng county.The positive rate of EHDV antibodies increased year by year from 2014 to 2016.There was an urgen need to strengthen the continuous study of EHDV infection and activity patterns,and improve the prevention and control efficiency of epidemic hemorrhagic disease.

Key words: epizootic hemorrhagic disease virus (EHDV); surveillance animals; infection rate; serotype

CLC Number:

S852.65

KOU Meiling, YANG Zhenxing, LI Le, ZHU Jianbo, GAO Lin, MIAO Haisheng. Serological Investigation and Serotype Identification of Epidemic Hemorrhagic Virus in Yunnan Border Area[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 3065-3074.

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Serological Investigation and Serotype Identification of Epidemic Hemorrhagic Virus in Yunnan Border Area

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