高浓度过瘤胃脂肪粉对绵羊葡萄糖和胰岛素耐量的影响

doi:10.16431/j.cnki.1671-7236.2024.04.013

Abstract

Abstract: 【Objective】 The purpose of this study was to explore the effects of high dose of rumen protected fat (RPF) on the growth performance, glucose dynamics and insulin resistance of sheep,so as to provide a scientific basis for the efficient utilization in ruminant nutrition.【Method】 A total of 160 3-months Hulunbuir sheep were selected and divided into 2 groups with 4 pens per group and 20 sheep per pen,which were named HF (23.65% RPF,16.34% corn) and CON (without RPF,36.95% corn) groups.The pre-feeding period of the feeding experiment was 14 days,and the experimental period was 42 days.The feed intake were recorded daily and the body weight of the sheep were measured every 21 days, and the average daily feed intake (ADFI), average daily gain (ADG) anf feed to gain ratio (F/G) were calculated. At the last day of the feeding period,4 sheep were randomly selected from different pens in each group,and they were subjected to intravenous glucose tolerance test (IVGTT),according to 0.25 g/kg BW,50% glucose solution was injected into the jugular vein.The serum from jugular vein blood was collected to measure the concentration of blood glucose and insulin,and the area under curve,maximum concentration,maximum concentration variation,insulin resistance index (HOMA-IR and Matsuda index) were calculated.【Result】 The ADFI and F/G of sheep in the HF group were significantly lower than those in CON group (P＜0.05).During the IVGTT,the serum glucose concentration of sheep in HF group at 2,10,30 min,and area under curve of glucose during the test were significantly lower than those in CON group (P＜0.05).The highest glucose concentration of sheep in HF group was obviously lower than that in CON group (P＜0.05).The insulin concentration at 10 and 30 min,and area under curve of insulin at 60 min in HF group were significantly lower than those in CON group (P＜0.05).The Matsuda index of HF group was obviously higher than that of CON group (P＜0.05).【Conclusion】 Adding high-concentration of RPF in the diet of growing sheep would not affect the growth performance of sheep, while could decrease the serum glucose concertration, and improve insulin sensitivity.

Key words: Hulunbuir sheep; rumen protected fat; glucose tolerance; insulin resistance

CLC Number:

S816.4

XU Tiantian, HU Fan, WANG Xue, WU Zhiwu, YE Bingsen, WEI Lingyun, HE Zhixiong, TAN Zhiliang. Effect of High Concentrated of Rumen Protected Fat on Glucose and Insulin Tolerance in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1448-1455.

References

[1] 王洪荣.反刍动物碳水化合物代谢利用机制及消化道健康的系统营养调控研究进展[J].动物营养学报,2020,32(10):4686-4696. WANG H R.Research advances in system-nutritional regulation on mechanism of carbohydrates metabolism and gastrointestinal health of ruminants[J].Chinese Journal of Animal Nutrition,2020,32(10):4686-4696.(in Chinese)
[2] 王洪荣.反刍动物瘤胃酸中毒机制解析及其营养调控措施[J].动物营养学报,2014,26(10):3140-3148. WANG H R.Mechanism analysis and nutritional strategies for prevention of sub-acute ruminal acidosis in ruminants[J].Chinese Journal of Animal Nutrition,2014,26(10):3140-3148.(in Chinese)
[3] LIU J,XU T,ZHU W,et al.High-grain feeding alters caecal bacterial microbiota composition and fermentation and results in caecal mucosal injury in goats[J].British Journal of Nutrition,2014,112(3):416-427.
[4] 周贵,陈红,万克军,等.关于高产奶牛饲料添加脂肪的探讨[J].吉林畜牧兽医,2002,6:10-12. ZHOU G,CHEN H,WAN K J,et al.Discussion on adding fat to dairy cattle feed[J].Jilin Animal Husbandry and Veterinary Medicine,2002,6:10-12.(in Chinese)
[5] OLIVEIRA D M,CHALFUN J A,CHIZZOTTI M L,et al.Expression of genes involved in lipid metabolism in the muscle of beef cattle fed soybean or rumen-protected fat,with or without monensin supplementation[J].Journal of Animal Science,2014,92(12):5426-5436.
[6] PATRA A K.A Meta-analysis of the effect of dietary fat on enteric methane production,digestibility and rumen fermentation in sheep,and a comparison of these responses between cattle and sheep[J].Livestock Science,2014,162(4):97-103.
[7] 韩文龙,周姝延,包凯,等.过瘤胃葡萄糖和过瘤胃脂肪预混剂对奶牛亚临床酮病治疗效果评价[J].黑龙江畜牧兽医,2014,5:138-140. HAN W L,ZHOU S Y,BAO K,et al.Evaluation of the therapeutic effect of rumen-protected glucose and rumen-protected fat premix on subclinical ketosis in dairy cows[J].Heilongjiang Animal Science and Veterinary Medicine,2014,5:138-140.(in Chinese)
[8] 袁雪,曲永利,韩云胜,等.不同类型过瘤胃脂肪对泌乳前期奶牛体况及生产性能的影响[J].中国畜牧杂志,2016,52(7):40-45. YUAN X,QU Y L,HAN Y S,et al.Effects of different types of rumen-protected fat on body condition and production performance of early lactation cows[J].Chinese Journal of Animal Science,2016,52(7):40-45.(in Chinese)
[9] 张海波.日粮补充棕榈酸对泌乳早期荷斯坦奶牛泌乳性能、血液生化指标和激素水平的影响[J].畜牧兽医学报,2019,50(1):203-210. ZHANG H B.Effects of dietary supplementation of palmitic acid on lactation performance,blood biochemical indexes and hormone concentration in early lactation Holstein dairy cows[J].Chinese Journal of Animal and Veterinary Sciences,2019,50(1):203-210.(in Chinese)
[10] 许鹏,杨致玲,曹名玉,等.饲粮添加不同类型及水平过瘤胃脂肪对荷斯坦奶牛生产性能及乳中脂肪酸组成的影响[J].动物营养学报,2022,34(3):1580-1591. XU P,YANG Z L,CAO M Y,et al.Effects of adding different types and levels of rumen-protected fat on performance and fatty acid composition of milk in Holstein dairy cows[J].Chinese Journal of Animal Nutrition,2022,34(3):1580-1591.(in Chinese)
[11] 王哲文,贺安祥,宋和键,等.过瘤胃脂肪对牦牛血清生化指标、内源激素、脂肪合成及代谢相关酶与肉品质的影响[J].动物营养学报,2023,35(2):968-976. WANG Z W,HE A X,SONG H J,et al.Effects of rumen protected fat on serum biochemical indices,endogenous hormones,enzymes related to fat synthesis and metabolism and meat quality in yaks[J].Chinese Journal of Animal Nutrition,2023,35(2):968-976.(in Chinese)
[12] CRUZ W F G D,SCHOONMAKER J P,RESENDE F D D,et al.Effects of maternal protein supplementation and inclusion of rumen-protected fat in the finishing diet on nutrient digestibility and expression of intestinal genes in Nellore steers[J].Animal Science Journal,2019,90(9):1200-1211.
[13] PERTIE J R,GUZIK T J,TOUYZ R M,et al.Diabetes,hypertension,and cardiovascular disease:Clinical insights and vascular mechanisms[J].The Canadian Journal of Cardiology,2018,34(5):575-584.
[14] EVERARD A,CANI P D.Diabetes,obesity and gut microbiota[J]. Best Practice & Research Clinical Gastroenterology,2013,27(1):73-83.
[15] KONGSTED A H,TYGESEN M P,HUSTED S V,et al.Programming of glucose-insulin homoeostasis:Long term consequences of pre-natal versus early post-natal nutrition insults.Evidence from a sheep model[J].Acta Physiologica,2014,210(1):84-98.
[16] ANTUNES L C,ELKFURY J L,JORNADA M N,et al.Validation of HOMA-IR in a model of insulin-resistance induced by a high-fat diet in Wistar rats[J].Archives of Endocrinology and Metabolism,2016,60(2):138-142.
[17] LIU A W,SONG S O,HAYASHI T,et al.Change in CT-measured abdominal subcutaneous and visceral but not thigh fat areas predict future insulin sensitivity[J].Diabetes Research and Clinical Practice,2019,154:17-26.
[18] DIOGÉNES L V,BEZERRA L R,PEREIRA FILHO J M,et al. Effects of the dietary inclusion of buriti oil on lamb performance,carcass traits,digestibility,nitrogen balance,ingestive behavior and blood metabolites[J].Animals,2020,10(11):1973.
[19] GHORBANI H,KAZEMI B M,HOSSEINYAZDI M,et al.Effects of various fat delivery methods in starter diet on growth performance,nutrients digestibility and blood metabolites of Holstein dairy calves[J].Animal Feed Science and Technology,2020,262:114429.
[20] OBICI S,FENG Z,MORGAN K,et al.Central administration of oleic acid inhibits glucose production and food intake[J].Diabetes,2002,51(2):271-275.
[21] MATTOS R.Effects of dietary fatty acids on reproduction in ruminants[J].Reviews of Reproduction, 2000,5(1):38-45.
[22] 刘凯.甜菜碱和过瘤胃脂肪对育肥羊生产性能和肌肉脂肪酸组成的影响[D].兰州:兰州大学,2016. LIU K.Effects of betaine and rumen protected fat on the performance and the muscle fatty acids composition of finishing sheep[D].Lanzhou:Lanzhou University,2016.(in Chinese)
[23] VAN C F,EZEQUIEL J M,AUREA A P,et al.Feeding behavior,nutrient digestibility,feedlot performance,carcass traits,and meat characteristics of crossbred lambs fed high levels of yellow grease or soybean oil[J].Small Ruminant Research,2016,137:151-156.
[24] 刘融,陶慧,张乃锋,等.饲粮中棕榈油脂肪粉添加水平对育肥期湖羊生长性能和营养物质消化代谢的影响[J].动物营养学报,2018,30(12):5013-5022. LIU R,TAO H,ZHANG N F,et al.Effects of palm oil powder supplemental level on growth performance and nutrient digestion and metabolism of finishing Hu sheep[J].Chinese Journal of Animal Nutrition,2018,30(12):5013-5022.(in Chinese)
[25] HILL S R,KNOWLTON K F,DANIELS K M,et al.Effects of milk replacer composition on growth,body composition,and nutrient excretion in preweaned Holstein heifers[J].Journal of Dairy Science,2008,91(8):3145-3155.
[26] HILL T M,BATEMAN H G,ALDRICH J M,et al.Effects of fat concentration of a high-protein milk replacer on calf performance[J].Journal of Dairy Science,2009,92(10):5147-5153.
[27] HADDAD S G,YOUNIS H M.The effect of adding ruminally protected fat in fattening diets on nutrient intake,digestibility and growth performance of Awassi lambs[J]. Animal Feed Science and Technology,2004,113(1):61-69.
[28] ROBERTS-THOMSON K M,PARKER L,BETIK A C,et al.Oral and intravenous glucose administration elicit opposing microvascular blood flow responses in skeletal muscle of healthy people:Role of incretins[J]. The Journal of Physiology,2022,600(7):1667-1681.
[29] 赵祥,杨美红,董宽虎,等.添加铬对肥育绵羊血清代谢物和免疫球蛋白含量的影响[J].动物营养学报,2005,17(2)::38-42. ZHAO X,YANG M H,DONG K H,et al.Effect of addition of chromium on the concentration of serum metabolites and immunoglobulin in finishing sheep[J].Chinese Journal of Animal Nutrition.2005,17(2):38-42. (in Chinese)
[30] LOHRENZ A K,DUSKE K,SCHNEIDER F,et al.Milk performance and glucose metabolism in dairy cows fed rumen-protected fat during mid-lactation[J].Journal of Dairy Science,2010,93(12):5867-5876.
[31] GRANDL G,STRAUB L,RUDIGIER C,et al.Short-term feeding of a ketogenic diet induces more severe hepatic insulin resistance than an obesogenic high-fat diet:A short-term ketogenic diet induces hepatic insulin resistance[J].The Journal of Physiology,2018,596(19):4597-4609.
[32] CASTALDO G,PALMIERI V,GALDO G,et al.Aggressive nutritional strategy in morbid obesity in clinical practice:Safety,feasibility,and effects on metabolic and haemodynamic risk factors[J].Obesity Research & Clinical Practice,2016,10(2):169-177.
[33] WATANABE M,TOZZI R,RISI R,et al.Beneficial effects of the ketogenic diet on nonalcoholic fatty liver disease:A comprehensive review of the literature[J].Obesity Reviews,2020,21(8):DOI:10.1111/obr.13024.
[34] GOWER B A,CHANDLER-LANEY P C,OVALLE F,et al.Favourable metabolic effects of a eucaloric lower-carbohydrate diet in women with pcos[J].Clinical Endocrinology,2013,79(4):550-557.
[35] HUSSAIN T A,MATHEW T C,DASHTI A A,et al.Effect of low-calorie versus low-carbohydrate ketogenic diet in type 2 diabetes[J].Nutrition,2012,28(10):1016-1021.
[36] HASAN-OLIVE M M,LAURITZEN K H,ALI M,et al.A ketogenic diet improves mitochondrial biogenesis and bioenergetics via the PGC1α-SIRT3-UCP2 axis[J].Neurochemical Research,2019,44(1):22-37.
[37] BURÉN J,ERICSSON M,DAMASCENO N R T,et al.A ketogenic low-carbohydrate high-fat diet increases LDL cholesterol in healthy,young,normal-weight women:A randomized controlled feeding trial:3[J].Nutrients,2021,13(3):814.
[38] JORNAYVAZ F R,JURCZAK M J,LEE H-Y,et al.A high-fat,ketogenic diet causes hepatic insulin resistance in mice,despite increasing energy expenditure and preventing weight gain[J].American Journal of Physiology—Endocrinology and Metabolism,2010,299(5):E808-E815.
[39] DOURIS N,MELMAN T,PECHERER J M,et al.Adaptive changes in amino acid metabolism permit normallongevity in mice consuming a low-carbohydrate ketogenic diet[J].Biochimica et Biophysica Acta—Molecular Basis of Disease,2015,1852(10):2056-2065.
[40] YUAN X,WANG J,YANG S,et al.Effect of the ketogenic diet on glycemic control,insulin resistance,and lipid metabolism in patients with t2 dm:A systematic review and Meta-analysis:1[J].Nutrition & Diabetes,2020,10(1):1-8.

Effect of High Concentrated of Rumen Protected Fat on Glucose and Insulin Tolerance in Sheep

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