多重PCR对猪病毒性繁殖障碍疾病的调查分析

Abstract

Abstract: In order to investigate the prevalence of porcine reproductive failure caused by viruses, 1068 patient samples from 14 pig farms in the vicinity of Taiyuan were detected using multiplex PCR diagnostic methods. The average infection, rate of porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSFV), porcine parvovirus (PPV), pseudorabies virus(PRV) and porcine circovirus type 2 (PCV-2) were 32%,40%,20%,12%,27%, respectively.More than two viruses mixed infection was 39%,two virus infection was 24%. 1572 blood samples were detected using RT-PCR kits for PRRSV RNA and CSFV RNA in 11 cities of Shanxi province from 66 pig farms and 121 free-range pig farm households. The results showed that PRRSV infection rate was 33%. CSFV infection rate was 46%.1593 blood samples were detected to indentify immune antibody titer of CSFV, the average qualified rate was 43.9%. It provides experimental data for working out comprehensive measures to prevent and control the epidemic diseases.

Key words: multiplex PCR; PRRSV; PPV; PRV; PCV-2; CSFV; reproductive failure

CLC Number:

S858.28

WANG Juan-ping;YAO Jing-ming;LEI Yu-ping;ZHOU Sheng-hua;DING Fu-xiang;CAO Ri-liang;WU Xin;SUN Jian-gang. Investigation and Analysis on Multiplex PCR for Porcine Reproductive Failure Caused by Viruses[J]. , 2009, 36(5): 152-156.

[1]	OU Yunwen, PAN Qin, WANG Yang, DAI Junfei, REN Shaoke, ZHANG Yang, ZHANG Jie. Truncated Expression of ORF2 Gene of Porcine Parvovirus Type 6 and Preparation of Its Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2487-2495.
[2]	KANG Pu, REN Zhaowen, CAI Rujian, LYU Zongji, HUANG Yuhao, WANG Xiaohu. Variation Regularity and Transmission of PRRSV-2 Lineage 8 Strains in Guangdong and Surrounding Provinces [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2001-2011.
[3]	CHEN Tianbao, WANG Yani, LIU Baoling, WANG Gang, WANG Xiaohu, LIU Xuhui, CAI Rujian, HE Dongsheng. Research Progress on the Anti-Porcine Reproductive and Respiratory Syndrome Virus Effect and Mechanism of Traditional Chinese Medicine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2114-2129.
[4]	ZHANG Jingxu, XU Yu, LIANG Haiying, ZENG Zhiyong, TANG Deyuan, WANG Bin, XU Songping, WAN Juan, ZHU Yang. Phylogenetic Evolution and Epitope Analysis of Pseudorabies Virus Guizhou Isolates and Vaccine Strains [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1093-1106.
[5]	LI Huawei, GUO Kewei, WANG Xuying, ZHANG Xiaoyu, LU Ziwei, LI Xinfeng, MA Hui, HOU Wenjing. Eukaryotic Expression and Subcellular Localization of Nonstructural Protein 7 of Porcine Reproductive and Respiratory Syndrome Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1160-1168.
[6]	MIAO Weiyuan, ZHANG Siqi, HUANG Changqiao, ZHAO Yi, CHEN Qi, XIE Xiaodong, WEI Yingyi, YU Meiling, HU Tingjun. Effect of N-butanol Fraction of Polygonum hydropiper Flavonoids on Levels Oxidative Stress-related Factors in 3D4/2 Cells Infected with PRV [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 349-358.
[7]	ZHANG Xinjie, XUE Shaohua, LYU Zixin, HUANG Xirong, CHEN Yuxuan, FAN Kewei, YANG Xiaoyan, DAI Ailing. Investigation of Co-infection of PPV7 and PCV2 and Genetic Variation Analysis for PPV7 Cap Gene in Fujian and Guangdong [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2291-2297.
[8]	XIE Sihao, GOU Hongchao, BIAN Zhibiao, LI Bin, CAI Rujian, ZANG Yingan, LI Chunling. Effects of MLKL Gene Knockout on Replication of Pseudorabies Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1934-1941.
[9]	LIU Qingqing, WU Peng, LI Peidong, ZHANG Jiangwei, CHEN Chuangfu, XIAO Chencheng. Construction of Recombinant Adenovirus Expressing Porcine Reproductive and Respiratory Syndrome Virus N Protein and Its Immunogenicity Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1024-1031.
[10]	WANG Zhaoli, YANG Siyu, WU Yanmei, SONG Xiaoxiao, MU Xiang, ZHANG Tao. Effects of PRRSV-LPS Treatment on Neutrophil Adhesion to Porcine Pulmonary Microvascular Endothelial Cells [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 471-479.
[11]	ZHANG Yanmin, ZHOU Yanan, CAO Lei, TIAN Yuan, LIU Xuping, TAN Wensong, ZHAO Liang. Expression and Immunogenicity Analysis of Classical Swine Fever Virus E2-GM-CSF Fusion Protein in HEK293T Cells [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 669-676.
[12]	SUN Lu, ZHANG Jing, YIN Miao, CHENG Yaodie, DAI Kun, WU Qian, ZHAO Hong, CHEN Xiwen. Study on the Antibacterial and Anti-PRRSV Activities of Four Heat Clearing and Detoxifying Compound Chinese Herbal Medicine in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 746-754.
[13]	LIU Huan, ZHANG Mengmeng, CUI Yifang, LIU Jing, ZHANG Jingju, CHEN Dengjin, LI Xiubo, LI Cun, XU Fei. Optimization of Foaming Performance of Compound Quaternary Ammonium Salt Foam Disinfectant and Evaluation of Its Killing Effect on Viruses [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4866-4877.
[14]	CHU Pinpin, JIANG Zhiyong, LI Chunling, GOU Hongchao, LI Yan, CAI Rujian. Antiviral Effect of Silver Nanoparticles on Porcine Reproductive and Respiratory Syndrome Virus in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4356-4363.
[15]	XING Jiayou, FAN Wenjie, WANG Yumin, LU Xiuxiang, SONG Yue, CHU Beibei, WANG Jiang, WANG Mengdi. Effect of Tamoxifen on the Proliferation of Porcine Pseudorabies Virus in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4503-4512.

Investigation and Analysis on Multiplex PCR for Porcine Reproductive Failure Caused by Viruses

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments