共表达AsiaⅠ型口蹄疫病毒P1-2A和3C基因重组山羊痘病毒的构建及鉴定

›› 2009, Vol. 36 ›› Issue (5): 87-91.

• 生物技术 • Previous Articles Next Articles

Construction and Identification of Recombinant Goatpox Virus Coexpressing the P12A and 3C Genes of FMDV

JU Hou-bin¹，ZHANG Qiang²，ZHOU Jin-ping¹，LIU Pei-hong¹，WEI Guang-sen²

(1.Shanghai Animal Disease Control Center, Shanghai 201103,China;2.Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-05-20 Published:2009-05-20
Contact: WEI Guang-sen

Abstract

Abstract: Transfer vector is considered important step in the construction of recombinant virus, based on the sequence analysis of the genome of goatpox virus, based on the KpnⅠof the pUC119-TK, transfer vector with the P7.5 controlled EGFP was constructed. After transfection in BHK21 cells infected with goatpox virus, the reporter gene expressed 48 hours later. The results lay foundation for recombinant vaccine development.

Key words: FMDV; goatpox virus; P12A gene; 3C gene; TK gene

CLC Number:

JU Hou-bin;ZHANG Qiang;ZHOU Jin-ping;LIU Pei-hong;WEI Guang-sen. Construction and Identification of Recombinant Goatpox Virus Coexpressing the P12A and 3C Genes of FMDV[J]. , 2009, 36(5): 87-91.

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Construction and Identification of Recombinant Goatpox Virus Coexpressing the P12A and 3C Genes of FMDV

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