猪垂体特异性转录因子1基因cDNA的克隆及序列分析

China Animal Husbandry & Veterinary Medicine ›› 2009, Vol. 36 ›› Issue (5): 92-94.

• 生物技术 • Previous Articles Next Articles

Cloning and Sequence Analysis of cDNA of Swine POU1F1 Gene

SUN Li-ya, SONG Cheng-yi,GAO Bo, ZHAO Qin,WANG Xiao-yan, ZHOU Zhi-hui,WANG Sheng-zhi, ZHOU Hui-yun,LI Bi-chun

(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China )

Received:1900-01-01 Revised:1900-01-01 Online:2009-05-20 Published:2009-05-20
Contact: SONG Cheng-yi

Abstract

Abstract: A pair of primers was designed to clone the POU1F1 cDNA according to published swine POU1F1 mRNA sequence. The total RNA was extracted from swine pituitary and the full length cDNA of swine POU1F1 was amplified by reverse transcription-polymerase chain reaction (RT-PCR). The PCR product with expected size of 876 bp was inserted into PTZ57R/T vector and sequenced. Finally the result sequence was analyzed by DNAStar and it demonstrated 99.7% homology with published sequence in GenBank. The cloned cDNA will be used for further function research of alternative splicing of swine POU1F1 Gene .

Key words: POU1F1; cDNA; clone; sequence analysis

CLC Number:

Q785

SUN Li-ya;SONG Cheng-yi;GAO Bo;ZHAO Qin;WANG Xiao-yan;ZHOU Zhi-hui;WANG Sheng-zhi;ZHOU Hui-yun;LI Bi-chun. Cloning and Sequence Analysis of cDNA of Swine POU1F1 Gene[J]. China Animal Husbandry & Veterinary Medicine, 2009, 36(5): 92-94.

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Cloning and Sequence Analysis of cDNA of Swine POU1F1 Gene

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