山羊痘病毒A6L基因的克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2015.08.008

Abstract

Abstract: To explore the molecular characteristics of protein A6 from goatpox virus (GTPV), genomic DNA was extracted from goatpox virus Gulang isolate.The specific primers were designed and used to amplify the A6L gene from the genomic DNA by PCR.A PCR product was ligated into pGEM-T Easy vector.After transformation into E.coli DH5α, the positive clones were sequenced and the sequences were analyzed with the bioinformatics softwares.The result showed that A6L gene sequence contained an open reading frame (ORF) of 1128 nucleotides and deduced protein consisted of 375 amino acids with the theoretical molecular weight of 43.68 ku and isoelectric point of 7.50.Analysis of secondary structure of protein A6 revealed that α-helix, β-strand and random coil were 53.30%, 10.24% and 39.46%, respectively.Analysis of multiple sequence alignment indicated A6L gene from different capripoxvirus isolates were highly conserved with identity of more than 97%.The present results laid the foundation for further studies of biological functions of protein A6 and interaction among the early proteins of capripoxvirus.

Key words: goatpox virus; A6L gene; gene cloning; sequence analysis

CLC Number:

S852.65

ZHAO Yin-long, WU Guo-hua, ZHU Hai-xia, WU Jian, YAN Xin-min, ZHAO Zhi-xun, LI Jian, WU Na, MU Xiao-feng, ZHANG Qiang. Cloning and Sequence Analysis of A6L Gene of Goatpox Virus[J]. , 2015, 42(8): 1973-1978.

References

[1] Bowden T R,Coupar B E,Babiuk S L,et al.Detection of antibodies specific for sheeppox and goatpox viruses using recombinant cap ripoxvirus antigens in an indirect enzyme-linked immunosorbent assay[J].J Virol Methods,2009,161(1):19-29.
[2] 陈轶霞.羊痘诊断技术研究进展[J].安徽农业科学,2008,36(34):15008-15009.
[3] Gershon P D,Black D N.A comparison of the genomes of capripoxvirus isolates of sheep,goats,and cattle[J].Virology,1988,164(2):341-349.
[4] Gershon P D,Black D N.A capripoxvirus pseudogene whose only intact homologs are in other poxvirus genomes[J].Virology,1989,172(1):350-354.
[5] Tulman E R,Afonso C L,Lu Z,et al.Genome of lumpy skin disease virus[J].Virol,2001,75:7122-7130.
[6] Betakova T,Wolffe E J,Moss B,et al.Regulation of vaccinia virus morphogenesis:Phosphorylation of the A14L and A17L membrane proteins and C-terminal truncation of the A17L protein are dependent on the F10L kinase[J].Journal of Virology,1999,73(5):3534-3543.
[7] Wu X,Meng X,Yan B,et al.Vaccinia virus virion membrane biogenesis protein A11 associates with viral membranes in a manner that requires the expression of another membrane biogenesis protein,A6[J].Journal of Virology,2012,86(20):11276-11286.
[8] Meng X,Wu X,Yan B,et al.Analysis of the role of vaccinia virus H7 in virion membrane biogenesis with an H7-deletion mutant[J].Journal of Virology,2014,87(14):8247-8253.
[9] Satheshkumar P S,Weisberg A,Moss B.Vaccinia virus H7 protein contributes to the formation of crescent membrane precursors of immature virions[J].Journal of Virology,2009,83(17):8439-8450.
[10] Maruri-Avidal L,Weisberg A S,Moss B.Vaccinia virus L2 protein associates with the endoplasmic reticulum near the growing edge of crescent precursors of immature virions and stabilizes a subset of viral membrane proteins[J].Journal of Virology,2011,85(23):12431-12441.
[11] Meng X,Embry A,Rose L,et al.Vaccinia virus A6 is essential for virion membrane biogenesis and localization of virion membrane proteins to sites of virion assembly[J].Journal of Virology,2012,86(10):5603-5613.
[12] Rodriguez J R,Risco C,Carrascosa J L,et al.Vaccinia virus 15-kilodalton (A14L) protein is essential for assembly and attachment of viral crescents to virosomes[J].J Virol,1998,72:1287-1296.
[13] Traktman P,Liu K,DeMasi J,et al.Elucidating the essential role of the A14 phosphoprotein in vaccinia virus morphogenesis:Construction and characterization of a tetracycline-inducible recombinant[J].J Virol,2000,74(8):3682-3695.
[14] Rodriguez J R,Risco C,Carrascosa J L,et al.Characterization of early stages in vaccinia virus membrane biogenesis:Implications of the 21-kilodalton protein and a newly identified 15-kilodalton envelope protein[J].J Virol,1997,71:1821-1833.
[15] Wolffe E J,Moore D M,Peters P J,et al.Vaccinia virus A17L open reading frame encodes an essential component of nascent viral membranes that is required to initiate morphogenesis[J].J Virol,1996,70(5):2797-2808.

Cloning and Sequence Analysis of A6L Gene of Goatpox Virus

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Zhishuai, SUI Zhiyuan, ZHANG Jihu, LI Qingjin, XING Feng. Cloning of CNP Gene and Its Tissue Expression Characteristics at Puberty in Dolang Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1402-1412.
[2]	SONG Chun, WANG Bolin, LI Mei, WEN Ming, WANG Kaigong, CHEN Changxiu, CHENG Zhentao. Isolation and Identification of Mycoplasma synoviae Guizhou Strain and Sequence Analysis of VlhA Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1516-1523.
[3]	WEI Yunfang, LI Feixiang, Xing Yun, Huang Qingguo, LI Judong, WAN Jiusheng, CHEN Fangliang. Study on Cloning, Bioinformatics Analysis and Spatio-temporal Expression of IGF2 Gene in Kunming Dogs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 866-875.
[4]	HE Xianming, REN Guangcai, YE Junxian, LAN Hong, LIU Yufu, XIONG Ting, YANG Zekun, XU Ting, CHEN Ruiai. Isolation and Identification of a Strain of Infectious Bursal Disease Virus and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1005-1014.
[5]	CAI Jie, WU Jiayi, PAN Yuanxia, HU Gaowei, ZHANG Linzhou. Isolation, Identification and Drug Resistance Analysis of Chryseobacterium sp. from Pseudosciaena crocea [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1135-1143.
[6]	HU Xiaobing, LIN Biaosheng, WANG Zhenwei, ZHENG Lijing. Isolation, Identification and Genomic Sequence Analysis of a Strain of Clostridium butyricum from Pigs Utilizing Lactic Acid [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 569-578.
[7]	KOU Meiling, MIAO Haisheng, LI Le, XIE Jiarui, LI Huachun, SONG Jianling. Isolation and Identification of Chuzan Virus from Palyam Serogroup Virus in Yunnan Border Area [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4401-4409.
[8]	ZHANG Kai, XIA Yuxin, WANG Liqiang, HOU Shaoyang, WEN Haiyan, GE Shihao, ZHOU Wanrong, JIANG Lili, FAN Zhaobin. Cloning,Bioinformatics and Tissue Expression Analysis of Galectin-1 Gene in White Leghorn Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3736-3745.
[9]	TAN Bin, WANG Chao, WANG Yan, ZHANG Shuqin. Isolation and Identification of Parvovirus from Palm Civets and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4012-4018.
[10]	YU Huimin, WU Pengjie, LI Nannan, TAN Jing, XU Shufa, WU Jiangli. Cloning, Bioinformatics Analysis and Prokaryotic Expression of GABARAP Gene in Apis cerana cerana [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 60-69.
[11]	LIU Jinrui, LI Zhonghui, JIANG Fangfang, MA Yila, LI Wenrong, LI Haiying. Cloning of PDGFD Gene and Its Expression in Different Adipose Tissue in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3138-3146.
[12]	ZHANG Junfang, SUN Jianfu, SUN Bin, TANG Lin, WANG Ying, WANG Enze, CUI Yan, LI Qiang, YAN Changguo, LI Xiangzi. Cloning and Bioinformatics Analysis of PPARγ Gene of Yanbian Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2695-2704.
[13]	WANG Zhiyan, SUN Yujie, XU Xin, HUANG Baicheng, TIAN Kegong. Isolation,Identification and Pathogenicity of Porcine Deltacoronavirus Strain HeN10 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2982-2989.
[14]	LI Nan, HE Yuwen, ZUO Yuanyuan, MENG Jinxin, XU Tiangang, WANG Jinglin. Analysis of Molecular Biological Characteristics of Structural Genes of Getah Virus (SZC30) Isolated from Midge [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1149-1160.
[15]	ZHANG Qi, CHEN Haotian, SHAN Jiabao, CHANG Rui, ZHANG Xue, WANG Yao, WANG Junwei, MA Bo. Cloning and Sequence Analysis of NS1 and VP1 Genes of Gosling Plague Virus from Jilin District [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1161-1169.