猪化脓隐秘杆菌的分离与鉴定

Abstract

Abstract: A bacterial which was Gram positive, slenderness and irregularity in appearance were isolated from a pig， its liver had purulence nodus and lungs and the spleen were haematocele. The isolated bacterium grew slowly in BSA, sheep’s blood agar and chocolate agar, and showed α-haemolysis in sheep’s blood agar. The results of 16S rDNA PCR sequence and API biochemistry identification showed that the isolated bacterium was Arcanobacterium pyogenes. The Arcanobacterium pyogenes caused 100% mouse death in 24~52 h, and cause rabbit death in 72 h by enterocoelia innoculation, it showed that it was virulent bacterium.

Key words: Arcanobacterium pyogenes; 16S rDNA; PCR; biochemistry identification

CLC Number:

S858.28

LU Xing-hua;HE Shi-cheng;TAN Zhi-xiang;LIU Dao-xin;QIU Li-xin;FAN Zhong-xin;. Isolation and Identification of Arcanobacterium Pyogenes of Swine[J]. , 2009, 36(5): 149-151.

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Isolation and Identification of Arcanobacterium Pyogenes of Swine

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