不同浓度的IGF-1对山羊毛囊干细胞体外培养的影响

›› 2009, Vol. 36 ›› Issue (5): 127-129.

• 遗传繁育 • Previous Articles Next Articles

Effect of Different Concentrations of IGF-1 on the Culture of Goat

SHI Ming-yan1，3, GAO Xue2, YANG Xue-yi1, DOU Zhong-ying3

(1.Life Science Department of Luoyang Normal College, Luoyang 471022, China; 2.Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 3.Shaanxi Branch of National Stem Cell Engineering Center, Northwest A&F University,Yangling 712100, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-05-20 Published:2009-05-20

Abstract

Abstract: Hair follicles were isolated from goat ear skin by 2.4 U/mL Dispase digestion and the bulges were separated with special fine needle and digested with 0.5 mg/mL trypsin plus 0.2 mg/mL EDTA. The results showed that cells were cultured in different concentrations of IGF-1, IGF-1 had a good effect on the goat hair follicle stem cells in vitro and the 10 ng/mL was best concentration.

Key words: hair follicle stem cells; colony-forming efficiency; IGF-1; in vitro culture

CLC Number:

Q813.1⁺1

SHI Ming-yan;;GAO Xue;YANG Xue-yi;DOU Zhong-ying. Effect of Different Concentrations of IGF-1 on the Culture of Goat[J]. , 2009, 36(5): 127-129.

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Effect of Different Concentrations of IGF-1 on the Culture of Goat

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