子宫蓄脓对犬子宫内膜组织中IGF-2及IGF-1R蛋白表达的影响

doi:10.16431/j.cnki.1671-7236.2016.05.022

Abstract

Abstract: To investigate the expression of IGF-2 and IGF-1R protein in endometria of canine pyometra and analyze the possible correlation with the incidence of canine pyometra.Immunohistochemistry PV method and Western blotting were used to measure the IGF-2 and IGF-1R expression characteristic of 5 normal endometria and 19 endometria of canine pyometra.The results showed that the IGF-2 level of endometria from pyometra group was extremely significant higher than that of control group by immunohistochemistry and Western blotting (P<0.01).The positive expression rate of IGF-1R in endometria of canine pyometra compared with that in the normal endometria was extremely significant higher by immunohistochemistry and Western blotting (P<0.01).The correlation analysis of IGF-2 and IGF-1R in canine pyometra showed that the result of immunohistochemistry was low correlation (ｒ=0.423), the result of Western blotting was high correlation (ｒ=0.927).The data suggested that increased expression of IGF-2 and IGF-1R played an important role in occurrence of canine pyometra.

Key words: IGF-2; IGF-1R; canine pyometra; immunohistochemistry; Western blotting

CLC Number:

S829.2

DU Miao, YUE Ying-wei, ZHOU Peng-yang, LI Shuang, MA Wen-zhi. Effect of Canine Pyometra on IGF-2, IGF-1R Proteins Expressions of Endometria[J]. , 2016, 43(5): 1263-1269.

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Effect of Canine Pyometra on IGF-2, IGF-1R Proteins Expressions of Endometria

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