老龄哈多利系博美犬睾丸组织结构分析

doi:10.16431/j.cnki.1671-7236.2019.11.008

Abstract

Abstract: This study was conducted to characterize the histologic structure of testicular tissue and the distribution of related protein between aging and young adult Hadley Pomeranian,and analyze the variation at different ages and influence on reproductive function.The testicular histochemical characteristics of young adult and agin Hadley Pomeranian were compared by special staining,immunohistochemistry and immunofluorescence.The quantitative analysis was carried out by IPP image analysis software.The results showed that compared with the young adult Pomeranian testis,the number and thickness of spermatogenic epithelial of the aging Pomeranian were decreased,and the number of Leydig cells and Sertoli cells were significantly increased (P<0.05).The basal layer of the seminiferous tubules and vascular wall were increased significantly.The results of immunohistochemistry showed that compared with young adult Pomeranian,the content of Col Ⅳ and HSPG in the aging Pomeranian testicular were extremely significantly decreased (P<0.01),the LN significantly decreased (P<0.05).Immunofluorescence results showed that Col Ⅳ,HSPG and LN were strongly expressed in Leydig cells and Sertoli cells.Therefore,the increased collagen and reticular fibers and the number of Leydig cells and Sertoli cells might be closely related to the spermatogenic function maintenance of the aging Pomeranian,and especially related to the Col Ⅳ and HSPG expression.

Key words: Hadley Pomeranian; aging; testis; immunohistochemistry; immunofluorescence

CLC Number:

S852.16

LI Chengye, GUO Yansheng, YUAN Ligang, WANG Qianmei, YANG Jie, REN Jianming, GAO Rui. Histological Characteristics Analysis of Aging Hadley Pomeranian Testis[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3198-3207.

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Histological Characteristics Analysis of Aging Hadley Pomeranian Testis

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