蓝舌病病毒VP7蛋白的原核表达及免疫原性鉴定

doi:10.16431/j.cnki.1671-7236.2015.08.012

Abstract

Abstract: To obtain VP7 protein of bluetongue virus (25 type), VP7 gene was amplified and cloned in pET-24b(+) expression vector.The pET-24b-BTV-VP7 recombinant plasmid was transformed into BL21 (DE3), then the VP7 protein of bluetongue virus was expressed using IPTG and purified by nickel affinity chromatography in vitro.Immunogenicity of VP7 protein was determined by Western blotting and ELISA.The results showed that the molecular weight of VP7 protein was about 40 ku and it could react with goat positive serum specifically.This study laid the foundation for establishing protein chip detection methods in the future.

Key words: bluetongue virus; prokaryotic expression; Western blotting; ELISA

CLC Number:

S852.65

JIA Yun, WANG Zhen-jun, SUN Ming-ying, ZHANG Xue, ZHANG Yong-ning, LUAN Shen-shun, WANG Quan-kai, SUN Ying-jie. Prokaryotic Expression and Immunogenicity Identification of Bluetongue Virus VP7 Protein[J]. , 2015, 42(8): 2000-2005.

References

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Prokaryotic Expression and Immunogenicity Identification of Bluetongue Virus VP7 Protein

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