梅花猪胃肠道5-羟色胺、胃饥饿素和胰高血糖素样肽-1免疫阳性细胞分布特征研究

doi:10.16431/j.cnki.1671-7236.2017.05.034

Abstract

Abstract:

In order to analyze the distributions and densities of endocrine cells in the gastrointestinal tract of Meihua pig (an elite native breed of Guangdong province, China), 6 healthy piglets weaned at 30 days of age were fed with the same diet till 65 days of age. At 65 days of age, different sections of the gastrointestinal tract of each piglet were collected, and the features and densities of 5-hydroxytryptamine (5-HT), ghrelin and glucagon-like peptide-1 (GLP-1) immunopositive cells were detected by immunohistochemistry. The results showed that the 5-HT positive cells distributed at the epithelial layer throughout the intestinal tract (duodenum, jejunum, ileum and colon) with a variety of features (e.g. cone, round, spindle), and they could be classified into either open-shape or closed-shape type, the density of 5-HT positive cells in duodenum was highest; The ghrelin positive cells were located along the gastrointestinal tract (gastric glands, pylorus, duodenum and jejunum) with both open-shape and closed shape, and its density decreased along the tract with most cells in gastric glands; The GLP-1 positive cells were observed along the intestinal tract, but its density increased from duodenum to ileum and then decreased in colon. This study might provide supporting data for further study of the gastrointestinal physiology of Meihua pig.

Key words: Meihua pig; gastrointestinal tract; 5-hydroxytryptamine(5-HT); ghrelin; glucagon-like peptide-1(GLP-1); immunohistochemistry

CLC Number:

S828.8⁺1

CHEN Zhong-jian, WU Xiu-ju, ZHU Cui, ZHANG Wei-na, LIANG Rui, YAN Shi-juan, SU Hao-xian, CHEN Zhuang. Distribution of 5-hydroxytryptamine, Ghrelin or Glucagon-like Peptide-1 Immunopositive Cells in the Gastrointestinal Tract of Meihua Pig[J]. , 2017, 44(5): 1498-1507.

References

[1] Mace O J,Tehan B, Marshall F.Pharmacology and physiology of gastrointestinal enteroendocrine cells[J]. Pharmacol Res Perspect,2015,3(4):e00155.
[2] Tolhurst G,Reimann F, Gribble F M.Intestinal sensing of nutrients[J]. Handb Exp Pharmacol,2012,209:309-335.
[3] Muscogiuri G,Cignarelli A,Giorgino F,et al.GLP-1:Benefits beyond pancreas[J]. J Endocrinol Invest,2014,37(12):1143-1153.
[4] Ariyasu H,Takaya K,Tagami T,et al.Stomach is a major source of circulating ghrelin, and feeding state determines plasma ghrelin-like immunoreactivity levels in humans[J]. J Clin Endocrinol Metab,2001,86(10):4753-4758.
[5] Kojima M,Hosoda H,Date Y,et al.Ghrelin is a growth-hormone-releasing acylated peptide from stomach[J]. Nature,1999,402(6762):656-660.
[6] Gershon M D.5-Hydroxytryptamine (serotonin) in the gastrointestinal tract[J]. Curr Opin Endocrinol Diabetes Obes,2013,20(1):14-21.
[7] Hoyer D,Hannon J P, Martin G R.Molecular, pharmacological and functional diversity of 5-HT receptors[J]. Pharmacol Biochem Behav,2002,71(4):533-554.
[8] Donovan M H, Tecott L H.Serotonin and the regulation of mammalian energy balance[J]. Front Neurosci,2013,7:36.
[9] 景栋林,王晔,邓日烈,等.梅花猪的生态分布及其品种特点[J]. 中国畜牧兽医,2006,33(7):38-39.
[10] 胡鸿惠,彭国良,肖正中.梅花猪品种资源的保护与遗传保种研究探讨[J]. 中国畜牧兽医,2015,42(4):1004-1008.
[11] Yano J M,Yu K,Donaldson G P,et al.Indigenous bacteria from the gut microbiota regulate host serotonin biosynthesis[J]. Cell,2015,161(2):264-276.
[12] Burkey T E,Skjolaas K A, Minton J E.Board-invited review:Porcine mucosal immunity of the gastrointestinal tract[J]. J Anim Sci,2009,87(4):1493-1501.
[13] Lam D D,Garfield A S,Marston O J,et al.Brain serotonin system in the coordination of food intake and body weight[J]. Pharmacol Biochem Behav,2010,97(1):84-91.
[14] Wang H,Steeds J,Motomura Y,et al.CD4⁺ T cell-mediated immunological control of enterochromaffin cell hyperplasia and 5-hydroxytryptamine production in enteric infection[J]. Gut,2007,56(7):949-957.
[15] Worthington J J.The intestinal immunoendocrine axis:Novel cross-talk between enteroendocrine cells and the immune system during infection and inflammatory disease[J]. Biochem Soc Trans,2015,43(4):727-733.
[16] Zietek T, Rath E.Inflammation meets metabolic disease:Gut feeling mediated by GLP-1[J]. Front Immunol,2016,7:154.
[17] Liou A P.Digestive physiology of the pig symposium:G protein-coupled receptors in nutrient chemosensation and gastrointestinal hormone secretion[J]. J Anim Sci,2013,91(5):1946-1956.
[18] 张晗,王磊,张涛,等.不同日龄猪小肠黏膜5-HT分布特征的研究[J]. 中国农业科学,2008,41(12):4218-4224.
[19] 刘霞,郭俊峰,褚春薇,等.贵州小型猪胃肠黏膜5-HT IR及SS-IR细胞的分布规律及形态学初步观察[J]. 黑龙江畜牧兽医,2015,11:125-127.
[20] 童川,岳晓慧,冯俊基,等.仔猪消化道胃泌素和五羟色胺分泌细胞时空分布的观察[J]. 中国农学通报,2010,26(10):5-8.
[21] 杨贵波,王平.改变食物组成对大鼠胃肠道嗜铬细胞的影响[J]. 动物学报,1997,43(1):55-60.
[22] 李洋洋,苏文,李淑兰,等.禁食对小鼠胃肠道5-羟色胺细胞的形态和分布密度的影响[J]. 黑龙江畜牧兽医,2016,4:37-39.
[23] Reynolds C B,Elias A N, Whisnant C S.Effects of feeding pattern on ghrelin and insulin secretion in pigs[J]. Domest Anim Endocrinol,2010,39(2):90-96.
[24] Du G M,Shi Z M,Wei X H,et al.Expression of gastric ghrelin and H⁺-K⁺-ATPase mRNA in weanling piglets and effect of ghrelin on H⁺-K⁺-ATPase expression and activity in gastric mucosal cellsin vitro[J]. Res Vet Sci,2007,82(1):99-104.
[25] Zhang H,Yin J,Li D,et al.Tryptophan enhances ghrelin expression and secretion associated with increased food intake and weight gain in weanling pigs[J]. Domest Anim Endocrinol,2007,33(1):47-61.
[26] Dong X Y,Xu J,Tang S Q,et al.Ghrelin and its biological effects on pigs[J]. Peptides,2009,30(6):1203-1211.
[27] Vitari F,Di Giancamillo A,Deponti D,et al.Distribution of ghrelin-producing cells in the gastrointestinal tract of pigs at different ages[J]. Vet Res Commun,2012,36(1):71-80.
[28] Rindi G,Necchi V,Savio A,et al.Characterisation of gastric ghrelin cells in man and other mammals:Studies in adult and fetal tissues[J]. Histochem Cell Biol,2002,117(6):511-519.
[29] Solcia E,Rindi G,Buffa R,et al.Gastric endocrine cells:Types, function and growth[J]. Regul Pept,2000,93(1-3):31-35.
[30] Janssen S,Laermans J,Iwakura H,et al.Sensing of fatty acids for octanoylation of ghrelin involves a gustatory G-protein[J]. PLoS One,2012,7(6):e40168.
[31] Eissele R,Goke R,Willemer S,et al.Glucagon-like peptide-1 cells in the gastrointestinal tract and pancreas of rat, pig and man[J]. Eur J Clin Invest,1992,22(4):283-291.
[32] 宋宇宏,姜吉文,欧叶涛,等.GLP-1-IR细胞在大鼠和成人肠道的分布[J]. 黑龙江医药科学,2001,24(3):3-4.
[33] 王俊波,李艳君,宁树君,等.GLP-1-IR细胞在鼠、猪和人胎儿肠道的分布[J]. 解剖科学进展,2000,6(2):140-143.
[34] Mace O J, Marshall F.Digestive physiology of the pig symposium:Gut chemosensing and the regulation of nutrient absorption and energy supply[J]. J Anim Sci,2013,91(5):1932-1945.

Distribution of 5-hydroxytryptamine, Ghrelin or Glucagon-like Peptide-1 Immunopositive Cells in the Gastrointestinal Tract of Meihua Pig

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Mingliang, ZHANG Hailong, MA Shipeng, WANG Cailian, LANG Xia. Effects of Zanthoxylum bungeanum Essential Oil on Production Performance and Gastrointestinal Tissue Structure of Small-tail Han Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 556-563.
[2]	ZHAO Fengmiao, GUO Ting, ZHOU Yaping, ZHAO Hongmei, WANG Yuchen, TIAN Guangyuan, SUN Yajie, BIAN Yuchen, YU Jialiang, HAO Yongqing. Pathogenicity Analysis of Bovine Viral Diarrhea Virus and Immune Effect Evaluation of E2 Protein in New Zealand White Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3549-3558.
[3]	LYU Xiaonan, XU Lipu, ZHANG Wen, WANG Na, CAO Huan, WANG Xiaoliang, WANG Shu, WANG Jingbo. Research on Histopathological Changes and Virus Distribution Situation of Mirror Carps Infected with Carp Edema Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1077-1084.
[4]	BU Ying, ZHENG Nan, WANG Jiaqi, ZHAO Shengguo. Research Progress on Immunoglobulin Regulating Gastrointestinal Health and Microbial Function in Animals [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3800-3808.
[5]	GUO Yan, DING Wei, TAO Jinzhong, CHEN Shaoyu, YUAN Ligang, LI Yingkang. Comparison of Skin Histochemical Characteristics in Tan Sheep at Different Ages [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2762-2770.
[6]	REN Jianming, WEI Yanming, YUAN Ligang, WANG Qianmei, LI Chengye. Comparison of the Distribution of PGP9.5 and NPY in Cryptorchidism and Testicular Tumors in Dogs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2741-2750.
[7]	ZHANG Yu, SHI Liguang, XUN Wenjuan, LIU Qiang. Research Progress on the Effects of Dietary Protein and Neutral Detergent Fiber Levels on Growth and Development of Young Ruminants [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2618-2624.
[8]	YU Wenjing, WANG Chao, WANG Min, ZHAO Shengguo, LIN Bo. Research Progress on the Effect of Sodium Butyrate on the Development of Gastrointestinal Tract of Calves [J]. , 2019, 46(8): 2354-2359.
[9]	ZHAO Fang, WANG Gen, ZHAO Guodong, GAO Chao, LI Xiaobin, MA Chen, YANG Kailun. Effect of Supplemented with Rumen-protected 5-hydroxytryptophan on Melatonin Content and Microbial Communities in Intestinal Tract Digesta of Sheep [J]. , 2019, 46(3): 690-702.
[10]	LI Chengye, GUO Yansheng, YUAN Ligang, WANG Qianmei, YANG Jie, REN Jianming, GAO Rui. Histological Characteristics Analysis of Aging Hadley Pomeranian Testis [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3198-3207.
[11]	MA Shuang, SONG Peng-yan, ZHAI Fu-zhan, ZHONG Xiu-hui. Effects of Maternal Exposure to Bisphenol A on Survival Rate, Reproductive Hormones and Relative Genes of Offspring Mice [J]. , 2017, 44(7): 2033-2041.
[12]	ZHANG Yu-yu, ZHAO Xin-lin, QI Feng-hua, LU Yan, SHI Xiao-yu, HAN Xiao-li, XU Chun-sheng. Study on the Distribution of Glial Fibrillary Acidic Protein in Intestine of Yellow Feather Broiler [J]. , 2017, 44(5): 1315-1320.
[13]	ZHANG Ke, LI Bi-bo, TONG Tao, CHEN Yu-lin. Anatomical Features and Changing Rule of Gastrointestinal Parameter in Shaanbei White Cashmere Goat at 0 to 56 days of Age [J]. , 2017, 44(5): 1338-1345.
[14]	Wuliyasu, Wuyahan, SHI Zhen-dan, Ailungaowa, Zhamuga, Wuyunsiqin, Enhemarite, Narenhua. Effect of Cryopreservation on the Expression of Tetraspanin CD9 in Sheep Oocytes [J]. , 2016, 43(8): 2104-2111.
[15]	DU Miao, YUE Ying-wei, ZHOU Peng-yang, LI Shuang, MA Wen-zhi. Effect of Canine Pyometra on IGF-2, IGF-1R Proteins Expressions of Endometria [J]. , 2016, 43(5): 1263-1269.