连续培养瘤胃模拟发酵的工作参数设置研究进展

doi:10.16431/j.cnki.1671-7236.2017.01.015

Abstract

Abstract:

In vitro culture method is an important way to research in ruminant dietetics, including batch and continuous culture methods. Because the continuous culture method can better simulate the conditions of fermentation in rumen, it was applied more widely. There are many operation parameters of rumen simulation technique involved in continuous culture, such as temperature, pH, dilution rate, the proportion of buffer to rumen fluid, stirring rate and way, meanwhile, different operation parameters and their combinations will have a great influence on the fermentation results. On the basis of summarizing the results of previous studies, the authors review the effects of different parameters on volatile fatty acid, dry matter disappearance rate, pH, enzyme activity, number of protozoa and microbial protein, in dual-flow continuous culture system in this paper.

Key words: in vitro culture; continuous culture system; operating parameters

CLC Number:

Q482

WU Meng-nan, SHEN Wei-jun, ZHANG Pei-hua, CHEN Dong. Research Progress on Working Parameters of the Continuous Culture of Rumen Simulation System[J]. , 2017, 44(1): 106-112.

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Research Progress on Working Parameters of the Continuous Culture of Rumen Simulation System

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