Abstract:

100 SD rats weighting 100～120 g were divided randomly into 3 groups. The animals in groupⅠwere fed with 3.0 mg/kg selenium as Se-enriched malt (SEM) supplemented diets. Animals in groupⅡand groupⅢreceived 0.1 mg/kg selenium in sodium selenite only and were severed as the positive control and negative control respectively. Animals groupⅠand groupⅡwere induced hepatoma by diethylnitrosamine resolved 〖JP+3〗with sterilized water (100 mg/L) at the dosage of 10 mg/kg body weight every day for 16 weeks，then drank with sterilized water for another 2 weeks. At 18th week，the rats were killed. Microvascular density (MVD) and.vascular endothelial growth factor (VEGF) protein level in tumor tissue were determined by immunohistochemistry. The results indicated that MVD and VEGF protein levels in the SEM group were significantly lower than those in the model group (P<0.05). It demonstrated that SEM could inhibit angiogenesis in the rat hepatoma. The down-regulating of VEGF expression might be involved in the angiogenesis inhibition.

Key words: selenium-enriched malt; diethylnitrosamine; rat hepatocarcinogenesis; angiogenesis