Abstract: Toxoplasmosis is a zoonotic parasitic disease, with a worldwide distribution, which can seriously hazard human health and make a large economic loss of animal husbandry in many countries. However, due to the deficiency of specific clinical sign, the diagnosis of toxoplasmosis becomes more difficult. The fast development of molecular techniques provide feasiblely quick, sensitive, specific and stable detection methods for diagnosing toxoplasmosis. This paper summarize the research advancement of molecular diagnosis technique from the following perspective: nucleic acid molecular hybridization, polymerase chain reaction（PCR）, monoclonal antibody（McAb）and gene chip．

Key words: Toxoplasma gondii; nucleic acid molecular hybridization; PCR; McAb; gene chip