Abstract:

For exploring genetic diversity of Juema pig, researched on the polymorphism of growth hormone gene order of Juema pig, and selected sequences in 14 kinds of pig from GenBank. Full sequence of growth hormone gene of 7 Juema pigs had been cloned successfully for the first time, from which 5 Juema pigs were sequenced respectively, whose polymorphism is intron 1 contains 1 commutation and 1 transversion, intron 2 contains 2 transversions, explain that the difference between Juma pigs may be connected with controlling of intron. The variation of 155 nucleotide all were detected in coding and noncoding region by comparing Juema pig with other 14 breeds, and 119 out of them were in intron regions and 28 out of them were in exon regions, with 104 transversions, 14 commutations, 23 absences, 14 inserts among them. Among substitutive sites of basic group, most was transversion mutations; the comparision between transversion and commutations was 7.43∶1, which exists transversion bias phenomenon. The percentage and divergence number of Juema pig’s growth hormone gene homology analyse showed that homology was nearer by comparing Juema pig with other minitype pig, homology of Juema pig and other large pig was farer. Dendrogram of growth hormone gene sequence in Juema pig etc. of 15 kinds analyse, which indicated Juema pig having far distant genetic relationship with the Vize pig; Juema pig and Rongjiang pig’s genetic relationships were nearest.

Key words: Juema swine; growth hormone gene; homology; polymorphism