雷州山羊TBX15基因多态性及其与体尺性状的关联分析

doi:10.16431/j.cnki.1671-7236.2024.09.021

Abstract

Abstract: 【Objective】 The aim of this study was to screen the potential single nucleotide polymorphism (SNP) of T-box transcription factor 15 (TBX15) gene and analyze its correlation with body size traits in Leizhou goats, so as to provide theoretical support for molecular breeding of Leizhou goats. 【Method】 DNA were extracted from blood samples of 78 Leizhou goats.TBX15 gene SNP was detected by PCR amplification and direct sequencing, and its genetic diversity was analyzed.The correlation between the polymorphism of TBX15 gene and body size traits such as body height, body length and chest circumference in Leizhou goats were analyzed using SPSS 23.0 software.The linkage disequilibrium and haplotype of TBX15 gene SNP were analyzed by HaploView software. 【Result】 Three SNPs were found on the intron of TBX15 gene, namely g.96346139 A＞G, g.96345894 T＞C and g.96330058 G＞A.There were three genotypes in all the three SNPs, and the dominant genotypes were GG, CC and AA, respectively.The chi-square test indicated that three SNPs were all in Hardy-Weinberg equilibrium, the polymorphic information content (PIC) were 0.309, 0.285 and 0.294, respectively, indicating moderate polymorphism (0.25＜PIC＜0.50).The association analysis results showed that the body height, cross height and body length of GG genotype at g.96346139 A＞G of TBX15 gene in Leizhou goats were significantly higher than those of AA genotype (P＜0.05).The body height, cross height, body length and chest depth of CC genotype at g.96345894 T＞C of TBX15 gene were significantly higher than those of TT genotype (P＜0.05).The chest depth of GG genotype at g.96330058 G＞A of TBX15 gene was significantly lower than that of the other two genotypes (P＜0.05).In the analysis of haplotypes and linkage disequilibrium, it was found that there was a strong linkage relationship among three SNPs (R²＞0.33), with five main haplotypes, among which the dominant haplotype was ACG, with a probability of 0.716. 【Conclusion】 Three SNPs of TBX15 gene in Leizhou goats had significant effects on body height, body length and other body size indicators, and the three SNPs were in linkage disequilibrium with a strong linkage relationship.Haplotype ACG could serve as candidate markers in Leizhou goat breeding.

Key words: Leizhou goats; TBX15 gene; body size traits; polymorphism; correlation

CLC Number:

S813.3

ZHOU Guangxian, TAN Jiayong, YANG Jian, LIU Yanfen, GAN Shangquan, ZHAO Zhihui, KANG Danju. Polymorphism of TBX15 Gene and Its Association Analysis with Body Size Traits in Leizhou Goats[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(9): 3930-3938.

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Polymorphism of TBX15 Gene and Its Association Analysis with Body Size Traits in Leizhou Goats

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