湖羊STAT5a基因第10内含子多态性及其与泌乳性状的关联分析

doi:10.16431/j.cnki.1671-7236.2023.09.024

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the polymorphic sites of signal transducer and activator of transcription 5A (STAT5a) gene intron 10 and its correlation with lactation traits,which provided reference basis for molecular marker breeding of Hu sheep.【Method】 Blood of 71 Hu sheep ewes were collected.The single nucleotide polymorphism (SNP) of STAT5a gene in Hu sheep was detected by PCR amplification and Sanger sequencing.SAS 9.4 software was used to analyze the correlation between SNP polymorphism of STAT5a gene and milk yield,milk fat percentage,milk protein percentage and somatic cell count of Hu sheep at 7-56 days.【Result】 One SNP locus was detected in STAT5a gene intron 10 of Hu sheep (g.41838147 A＞G),and AA,AG and GG genotypes were found in this locus.Chi-square test showed that the mutation site of g.41838147 A＞G deviated from Hardy-Weinberg equilibrium (P<0.05).The polymorphism information content (PIC) calculation showed that the g.41838147 A＞G site was moderately polymorphic (0.25<PIC<0.50).The results of association analysis showed that the GG genotype of g.41838147 A＞G locus was extremely significantly or significantly higher than that of AA and AG genotypes (P<0.01 or P<0.05),and the average lactose rate was significantly higher than that of AA genotype (P<0.05).The average somatic cell count of AG and GG genotypes was significantly lower than that of AA genotype (P<0.05).【Conclusion】 There was one polymorphism locus in STAT5a gene intron 10,which was significantly associated with total milk yield,average lactose rate and average somatic cell count of Hu sheep.

Key words: Hu sheep; STAT5a gene; intron 10; lactation traits; correlation analysis

CLC Number:

S813.3

LIU Yu, ZHANG Linlin, FANG Yi, ZHANG Jinlong, LI Yihai, ZHONG Rongzhen, SHENG Jiahai, HE Yongxiang, GUO Xiaofei, ZHANG Xiaosheng. Polymorphism of STAT5a Gene Intron 10 and Its Association with Lactation Traits in Hu Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3680-3687.

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Polymorphism of STAT5a Gene Intron 10 and Its Association with Lactation Traits in Hu Sheep

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