Abstract: According to GenBank Salmonella invasion protein A (invA) gene sequences, a pair of primers were designed for amplification of specific nucleic acid fragment of 202 bp, after optimizing the PCR amplification conditions, established a Salmonella-specific, sensitive and rapid PCR detection method. Specificity test results showed that Salmonella strains are able to amplify the specific nucleic acid fragment, the results of E.coli, Pasteurella multocida, Staphylococcus aureus, Shigella, Bacillus cereus, Proteus mirabilis, Pseudomonas aeruginosa were negative. Sensitivity test results showed that the sensitivity of the method can reach 2.43×10³ CFU/mL.

Key words: Salmonella; PCR; detection