载脂蛋白CⅢ刺激猪主动脉血管内皮细胞差异表达基因的研究

doi:10.16431/j.cnki.1671-7236.2016.03.004

Abstract

Abstract: The aim of this study was to investigate the differential expression genes induced by ApoCⅢ,and study the function of ApoCⅢ.Porcine aortic vascular endothelial cells were successfully isolated using enzyme digestion,and then screened the differential expression genes induced by ApoCⅢ using the Solexa high-throughput sequencing technology.The results showed 647 differential expression genes,including 390 up-regulated genes and 257 down-regulated genes.The qRT-PCR results verified that the gene expression results from Solexa sequencing data were reliable.GO and Pathway analysis showed that the function of differential expression genes were related to immune response,cell apoptosis and death.These findings suggested that ApoCⅢ affected the physiological function of porcine aortic endothelial cells by the molecular pathways of inflammation,cell adhesion and apoptosis,which provided a theoretical basis for further understanding the molecular mechanisms of atherosclerosis caused by ApoCⅢ.

Key words: ApoCⅢ; porcine aortic vascular endothelial cell; high-throughput sequencing

CLC Number:

Q786

YUE Yuan, CHEN Hong-yan, WANG Jia-wei, XU Ming-qiang, DING Yu, JIANG Hao, GAO Yan, ZHANG Jia-bao, YAN Shou-qing. Analysis on Differential Expression Genes in Porcine Aortic Vascular Endothelial Cells Induced by Apolipoprotein C Ⅲ[J]. , 2016, 43(3): 585-591.

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Analysis on Differential Expression Genes in Porcine Aortic Vascular Endothelial Cells Induced by Apolipoprotein C Ⅲ

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