萨能奶山羊胎儿成纤维细胞miRNA文库的构建及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2020.11.003

Abstract

Abstract: The purpose of this experiment was to enrich miRNA library of goat and explore the role of miRNAs in the regulation of cell growth.The expression of Saanen dairy goat fetal fibroblast miRNAs was analyzed by Illumina high-throughput sequencing and bioinformatics technology.Total miRNAs cDNA library from fibroblasts of Saanen dairy goat was successfully constructed,16 395 039 total reads and 16 150 181 clean reads had been obtained and among them were 205 857 unique sRNAs.From those clean reads,247 novel miRNAs were identified and 8 401 target genes were predicted with 10 832 target sites.Meanwhile,the first base pairs of candidate miRNAs were biased for U and A.GO analysis revealed that more than 69.6% of the genes were related to cells and cellular components,more than 54.1% of the genes were involved in organelle related activity,and approximately 65.0% of the genes were related with cells and cellular processes.KEGG pathway analysis showed that approximately 10.5% of the genes were related to a metabolic pathway,and that it was the pathway that accounts for the most.In conclusion,the data showed that miRNAs played an important regulatory role in fetal fibroblasts.The results provided a basis for further research on the growth regulation of fetal fibroblasts and miRNAs of donor cells in the breast bioreactor of dairy goats.

Key words: fetal fibroblast; miRNA; high-throughput sequencing; GO annotation; KEGG pathway

CLC Number:

CHANG Weihua, WANG Juanhong, CUI Zilong. Construction of miRNA Library and Bioinformatics Analysis About Fetal Fibroblasts of Saanen Dairy Goat[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3436-3444.

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Construction of miRNA Library and Bioinformatics Analysis About Fetal Fibroblasts of Saanen Dairy Goat

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