NF-κB信号通路调控布鲁氏菌胞内存活分子机制的初步研究

doi:10.16431/j.cnki.1671-7236.2016.03.005

Abstract

Abstract: By the infection of Brucella virulent strain and attenuated strain in mice macrophage RAW264.7,the assay was aimed to explore the relationship between NF-κB signaling pathways and Brucella virulent strain and attenuated strain in intracellular survival.Use different MOI Brucella (2308,RB51,16M and M5) to infect mice macrophage RAW264.7,after 0,4,8 and 24 h infected,cracking cell and collecting supernatant,we detected the effect of Brucella on activation of NF-κB signaling pathway by Western blotting.Different concentrations of NF-κB signaling pathway inhibitor were incubated with mice macrophage RAW264.7,with different multiplicities of infection (MOI) of Brucella infecting cells,ELISA kits to detect the expressions of TNF-α,IL-1β and IL-6 cytokine;At the same time,count the number of intracellular bacteria of CFU.The results showed that rough cattle Brucella strains RB51 could strongly activate NF-κB signaling pathway,smooth cattle Brucella strains 2308 was weak in the activation;At the same time,the activation of NF-κB signaling pathway was concentration dependent.When the MOI was 80,infection time was 8 h,NF-κB activation degrees of rough cattle Brucella strains RB51 and smooth cattle Brucella strains 2308 were the strongest,and this pathway was involved in producing TNF-α and IL-6;NF-κB signaling pathway inhibitor BAY11-7082 affected Brucella intracellular survival.So rough cattle Brucella strains RB51 intracellular survival and NF-κB signaling pathway activity were closely related.The results laid the foundation for the further study of Brucella intracellular pathogenesis,also provided scientific basis for the research of new drugs to Brucella,and prevention and treatment of brucellosis.

Key words: Brucella 2308; Brucella RB51; macrophages; NF-κB signaling pathway; intracellular survival

CLC Number:

S852.61⁺4

YI Ji-hai, ZHANG Jun-bo, LI Shuang, WANG Xi-chu, ZHANG Hui, CHEN Chuang-fu. Preliminary Study on NF-κB Signaling Pathway Regulating Brucella Intracellular Survival Molecular Mechanisms[J]. , 2016, 43(3): 592-600.

References

[1] 任洪林,卢士英,周玉,等.布鲁氏菌病的研究与防控进展[J].中国畜牧兽医,2009,36(9):139-143.
[2] Lenntte E H,Balows A,William J,et al.Manual of Clinical Microbiology[M].Washington DC:American Society for Microbiology,1985.
[3] Riley L K,Robertson D C.Brucellacidal activity of human and bovine poly morphonuclear leukocyte Granule extracts against smooth and rough strains of Brucella abortus[J].Infect Immun,1984,46(1):231-236.
[4] 王玉飞,陈泽良,白耀霞,等.布鲁氏菌vjbR突变株的构建及其毒力表型分析[J].微生物学通报,2010,37(8):1188-1193.
[5] 严明,陈万涛.NF-κB 信号转导通路和肿瘤发生、转移关系的研究进展[J].北京口腔医学,2007,1:53-55.
[6] 张彬,肖献忠张文辉,等.核仁素对LPS诱导的白细胞介素1β释放的影响[J].中国病理生理杂志,2010,9:1796-1800.
[7] Karin M,Greten F R.NF-kappaB:Linking inflammation and immunity to cancer development and progression[J].Nat Rev Immunol,2005,5:749-759.
[8] 邓文星,李芳,苏益琼,等.浅谈布鲁氏菌疫苗的研究进展[J].中国畜禽种业,2012,4:42-43.
[9] 陆承平.兽医微生物学[M].第3版.北京:中国农业出版社,2005.
[10] Mariani F,Cappelli G,Riccardi G,et al. Mycobacterium tuberculosis H37Rv comparative gene-expression analysis in synthetic medium and human macrophase[J].Gene,2000,253(2):281-291.
[11] Castañeda-Roldán E I,Avelino-Flores F,Dall'Agnol M,et al.Adherence of Brucella to human epithelial cells and macrophages is mediated by sialic acid residues[J].Cell Microbiology,2004,6(5):435-445.
[12] Kuroda M,Wilson T H,Tsuchiya T.Regulation of galactoside transport by the PTS[J].J Mol Microbiol,Biotechnol,2001,3(3):381-384.
[13] Ryan S,Taylor C T,McNicholas W T.Systemic inflammation:A key factor in the pathogenesis of cardiovascular complications in obstructive sleep apnoea syndrome[J].Thorax,2009,85(1010):693-698.
[14] Plommet M,Plommet A M.Virulence of Brucella:Bacterial growth and decline in mice[J]. Ann Rech Vet,1988,19(1):65-67.
[15] Forestier C,Moreno E,Pizarro-Cerda J,et al.Lysosomal accumulation and recycling of lipopolysaccharide to the cell surface of murine macrophages,an in vitro and in vivo study[J].J Immunol,1999,162(11):6784-6791.
[16] Forestier C,Deleuil F,Lapaque N,et al.Brucella abortus lipopolysaccharide in murine peritoneal macrophages acts as a down-regulator of T cell activation[J].J Immunol,2000,165(9):5202-5210.

Preliminary Study on NF-κB Signaling Pathway Regulating Brucella Intracellular Survival Molecular Mechanisms

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZENG Dongdong, LIU Caixia, KOU Lijun, QIN He, JIN Ruijie, WANG Jing, REN Jingjing, MA Xun, JIANG Jianjun. Construction and Biological Characteristics of Lm4b_02324 Gene Deletion Strains of Listeria monocytogenes [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1981-1990.
[2]	QIU Runhui, GUAN Feihu, WANG Zihang, GUO Jia, ZHU Dexin, ZHANG Wei, WEI Chunyan, HUO Mingkai, SUN Zhihua, ZHANG Hui. Construction and Biological Characteristics of Brucella Secreting Protein BMCO Gene Deletion Strain [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1630-1640.
[3]	LIU Qian, GUO Zhiting, ZHANG Kang, WANG Xuezhi, ZHANG Jingyan, LI Jianxi. Research Progress on Astragalus Polysaccharide on Innate Immunalregulation of Macrophage [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1746-1756.
[4]	ZHANG Yuzhu, YU Chao, ZHENG Huihua, TANG Xinyue, HUANG Ronglei, XIE Guanghong. Mechanism Study of Protocatechuic Acid on LPS-induced Endometritis in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1585-1591.
[5]	WANG Yueli, SHAO Zhiran, YI Jihai, WANG Yong, WANG Zhen, CHEN Chuangfu. miR-145a-3p Regulates Brucella Induced Mouse Macrophage RAW264.7 Autophagy by Targeting ATG14 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1117-1125.
[6]	QIU Shulei, CHEN Xiaolan, YANG Haifeng, CHEN Yuku, HU Yuanliang, GAO Yongxu, WU Caihong. Effects of Selenized Garlic Polysaccharide on the Function of Mouse Peritoneal Macrophages [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1144-1152.
[7]	YANG Yu, GUAN Wenchao, CUI Xiaozhen, JIA Yongxin, YANG Shiyu. Effects of Licochalcone A on Oxidative Stress Induced by Haemophilus parasuis Infection in Porcine Alveolar Macrophages [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 480-487.
[8]	XIE Shanshan, YANG Qin, DENG Xiaoyu, YI Jihai, WANG Zhen, CHEN Chuangfu. Effects of Mitochondrial Fusion Protein 2 Gene Interference and Overexpression on Macrophage Apoptosis Induced by Brucella [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 283-293.
[9]	ZHANG Jing, QIAN Yinghong, ZHANG Kai, WU Jindi, LIU Bo, MAO Wei, CAO Jinshan. Effects of Staphylococcus aureus Lipoprotein on Immunity in M1 Mouse Bone Marrow-Derived Macrophages [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 3079-3086.
[10]	LIU Haiyao, LI Na, ZHANG Xiaoguang, LI Ziheng, BAO Chuntong, JIANG Hexiang, LIU Baijun, XIAO Jiameng, WANG Jun, LI Fengyang, LEI Liancheng. Role of S100A12 in the Expression of Inflammatory Cytokines and Phagocytosis in Porcine Alveolar Macrophages Induced by Actinobacillus pleuropneumoniae [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4262-4273.
[11]	YANG Qin, DENG Xiaoyu, ZHAO Xiaoli, ZHANG Huan, XI Jing, YI Jihai, WANG Yong, ZHANG Qian, WANG Zhen, CHEN Chuangfu. Effects of Brucella Type Ⅳ Secretion System on Autophagy,Intracellular Survival and Inflammatory Response of Dendritic Cells [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 257-264.
[12]	DENG Xiaoyu, HE Jinke, YANG Qin, YI Jihai, WANG Yueli, XI Jing, WANG Zhen, CHEN Chuangfu. Construction of Brucella abortus A19 VirB Promoter Deletion Strain and Study on Its Biological Characteristics [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 864-872.
[13]	CHA Jinlong, LIU Chaoying, GAO Hong, WANG Shiyu, SHAN Chunlan, JING Linxi, WANG Hao, YANG Wei. Effect of Pathogenic E.coli HPI on the Expression of Burnout-related Molecules in Caspase-1 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3181-3189.
[14]	YI Jihai, WANG Yueli, WANG Zhen, CHEN Chuangfu. Research Progress on the Relationship Between Intracellular Survival Mechanism of Brucella and Macropohage Polarization [J]. , 2018, 45(4): 1082-1088.
[15]	LI Qi-feng, DING Jin-hua, DOU Wen-jing, ZHANG Hui, CHEN Chuang-fu. Effect on Expression of SUMO-1 in Cells Infected by Brucella and Construction of Lentivirus Vector that Over-expressing SUMO-1 [J]. , 2016, 43(6): 1422-1429.