西南矮马肌肉生长抑制素基因启动子区PCR-RFLP 多态性检测

Abstract

Abstract: In this present study, promoter region of myostation(MSTN) gene of Guizhou pony, Debao pony and Ningqiang pony were analyzed by PCR-RFLP. The results indicated that there was one SspⅠdigestion site in the 204 bp amplified fragment. An T→C mutation at the site 156 bp of this gene by DNA sequencing which produced two sorts of genotypes, AA and AB, however, BB genotype was not founded in this study. The genotype frequency of AB in Guizhou pony and Ningqiang pony were higher than AA, while AA genotype was preponderant in Debao pony. The allele frequency of A were higher than B in all these three pony breeds. Guizhou pony and Debao pony were at Hardy-Weinberg equilibrium (P＞0.05), but Ningqiang pony was significantly different (P＜0.05).

Key words: Southwest pony; MSTN gene; PCR-RFLP; polymorphism

CLC Number:

XU Long-xin, YANG Sheng-lin, YANG Hai-bing, LI Ai-ping, WANG Zhong-xia. PCR-RFLP Polymorphism of Promoter Region of MSTN Gene in Southwest Pony[J]. .

References

1 王全喜,红海,芒来,等. 蒙古马MSTN基因第三外显子的克隆及其SSCP研究[J]. 华北农学报, 2005,20(3):14~16.

2 王全喜,红海,芒来,等. 蒙古马肌生成抑制基因(MSTN)的克隆及序列分析[J]. 华北农学报,2006,21(5):45~49.

3 王振山,岳高峰. 中国矮马血液遗传标记的初步研究[J]. 黑龙江畜牧兽医,2002(10):3~5.

4 巨立中,成军,钟彦伟. 启动子DNA结合蛋白研究策略[J]. 世界华人消化杂志,2004,12(1):141~142.

5 李聚才,张春珍,杨易,等. 宁夏肉牛杂交改良群体肌肉生长抑制素基因多态性分析[J]. 中国畜牧兽医,2012,39(4):148~152.

6 姜运良,李宁,吴常信,等.不同品种猪肌肉生长抑制素基因单核苷酸多态性分析[J].遗传学报,2001,28(9):840~845.

7 姜运良,李宁,杜立新,等. 猪肌肉生长抑制素基因5'调控区T→A突变与生长性状的关系分析[J].遗传学报, 2002, 29(5):413~416.

8 熊恩国,朱开林,游兰韶,等.湖南省桑植县及天平山自然保护区蝶类[J].湖南农业大学学报,1999,25(4):312~317.

9 Dunner S, Miranda M E, Amigues Y, et al. Haplotype diversity of the myostatin gene among beef cattle breeds[J]. Genet Sel Evol, 2003, 35(1):103~118.

10 Klauzinska M,Siadkowska E,Grochowska R,et al. Polymorphism of ,olecular-genetic systems in the Polish Red cattle[J]. Tsitol Genet, 2001, 35(1):58~60.

11 Lee S J, McPherron A C. Myostatin and the control of skeletal muscle mass [J]. Curr Opin Genet Dev, 1999, 9(5): 604~607.

12 Marchitelli C, Savarese M C, Crisa A, et al. Double muscling in marchigiana beef breed is caused by a stop codon in the third exon of myostatin gene [J].Mamm Genome, 2003,14(6):392~395.

13 Mcpherron A C, Lawler A M, Lee S J. Regulation of skeletal muscle mass in mice by a new TGF-β superfamily member[J]. Nature, 1997,387:83~90.

14 Oblap R V, Zvezhkhovski L, Ivanchenko E V, et al. Comparative analysis of the genetic structure of Red Polish cattle in Poland and the Ukraine[J]. Tsitol Genet, 2002, 36(2):35~43.

15 Smith J A,Lewis A M,Wiener P,et al.Genetic variation in the bovine myostatin gene in UK beef cattle: Allele frequencies and haplotype analysis in the South Devon[J].Animal Genetic,2000,31(5):306.

16 Dall’Olio S, Fontanesi L, Costa L N, et al. Analysis of horse myostatin gene and identification of single nucleotide polymorphisms in breeds of different morphological types[J]. Journal of Biomedicine and Biotechnology, 2010, 2010: 39~49.

[1]	WANG Zeping, WANG Feifei, ZHANG Ruiming, FU Yanfeng, ZHAO Weimin, DAI Chaohui, CHENG Jinhua, LIAO Chao, LI Hui. Analysis on Polymorphism of SLA-1 Gene and Its Disease Resistance Potential in Suzi Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1918-1927.
[2]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.
[3]	TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.
[4]	LIU Zelin, YANG Juan, XU Mingzhu, ZHANG Nian, XIONG Qi, LIU Jingbo, TAO Hu. Association Analysis Between Polymorphisms of ZBP1 Gene and Lambing Performance in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 161-173.
[5]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[6]	XU Haoqi, XU Jingyi, HU Lirong, ZHANG Fan, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, LIU Qiaoxiang, GUO Gang, WANG Yachun. Polymorphism of PIK3CB Gene and Its Association Analysis with Reproductive and Milk Production Traits in Chinese Holstein Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3438-3452.
[7]	DING Mengqin, HEIDAXI·Bahati, PENG Fengqiang, HU Yali, SHAERSHANBIEKE·Abudilida. Analysis of Polymorphisms of FSHR Gene and Its Association with Production Performance of Baicheng You Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3044-3053.
[8]	ZHOU Di, AO Ye, JIANG Guirong, ZHAO Zhonghai, LI Jun, YANG Rong, GONG Fei, CHEN Changxue, SHEN Yin, LI Hui. Polymorphism of MEF2C Gene Exon and Its Association Analysis with Weight and Body Size Traits in Qiandongnan Xiaoxiang Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2613-2621.
[9]	MA Yanru, YU Yongsheng, CAO Yang, QIN Lihong, ZHAO Yumin, WU Jian. Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2186-2194.
[10]	GAO Beiyao, LIU Yanguang, JIA Qi, LIU Jianqiang, LUO Xinhui, ZHANG Lichun. Study on Polymorphism and Differential Expression of PBD-124 Gene in Different Breeds of Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2208-2215.
[11]	CHAI Zhixin, WU Zhijuan, WANG Jikun, ZHANG Chengfu, ZHONG Jincheng, XIN Jinwei. Polymorphisms of CTGF and Gprin3 Genes and Their Association with Body Size Traits in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2216-2227.
[12]	RUAN Yong, CHEN Xiang, DAI Lingang, HUANG Jiajin, AN Dongwei, ZOU Qishun. Correlation Analysis of VNN1 Gene Polymorphism and Lambing Number in F3 Generation Boza Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1384-1392.
[13]	ZHANG Yonghao, XIAO Yi, GUO Lijun, Tuerxun·Abudoureheman, WANG Yutao. Genetic Polymorphism Analysis of MHC-DRB3 Gene Exon 2 in Ovis ammon polii and Five Domestic Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 962-972.
[14]	LI Mingming, WU Qingwei, DENG Yuting, JIN Shengwei, HE Na, ZHANG Junxia. Correlation Analysis of BMPRⅡ Gene Polymorphism and Its Haplotype with Lambing Traits in Tibetan Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 587-597.
[15]	WANG Yingtong, YANG Suan, TAN Yuancheng, WANG Chunyuan, ZHANG Yiyu. Polymorphism of SLC8A1 Gene and Its Effect on Eggshell Quality in Changshun Blue-eggshell Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4688-4696.

PCR-RFLP Polymorphism of Promoter Region of MSTN Gene in Southwest Pony

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments