松辽黑猪H-FABP基因位点多态性研究

Abstract

Abstract: In order to detect the polymorphism of heart fatty acid-binding protein (HFABP)in the SongLiao black pig , genetic variations of 5’-upstream region and the second intron in 62 SongLiao black pigs H-FABP gene were checked by PCRRFLP with HinfI, HaeIII and MspI.The results indicate that SongLiao black pig only shows sigle AA genotype in MspI-RFLP, for HinfI-RFLP, SongLiao black pig had polymorphism and frequencies of allele H is 0.7177, and for HaeIII-RFLP, SongLiao black pig had polymorphism and frequencies of allele d is 0.6210.So the experiment indicates that SongLiao black pig has polymorphism in the 5’-upstream region and the second intron of H-FABP gene.

Key words: songliao black pig; H-FABP gene; polymorphism; PCR-RFLP

CLC Number:

Q754

GAO Yan;XIAO Shuqi;LI Yi;ZHAO Zhongli;YUAN Bao;ZHANG Jiabao;ZHAO Zhihui. Study on the Polymorphism of Songliao Black Pig Heart Fatty Acid-Binding Protein Gene[J]. , 2007, 34(3): 63-66.

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Study on the Polymorphism of Songliao Black Pig Heart Fatty Acid-Binding Protein Gene

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