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Table of Content
20 September 2008, Volume 35 Issue 9
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动物营养与饲料科学
Application Research of Rumen Protected Amino Acid on Dairy Cows Production
XIA Nan;WANG Jiaqi;ZHAO Guoqi;BU Dengpan;ZHANG Leying;WEI Hongyang;ZHOU Lingyun
2008, 1(9): 5-9.
Abstract
(
2503
)
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Lys and Met are most limiting amino acids on lactating dairy cow. However, it is impossible to achieve ideal performance through traditional methods which feeding high protein feedstuffs or free Met and Lys. Now, the focus of research has being transferred to rumen protected amino acid. The author reviewed the definition, measures, effects and influencing factors of rumen protected amino acid, as well its application in the future.
Effect of Different Forages Quality on Nutrient Digestibility and Growth in Calves Weaning to 7-monthold
CUI Lihong;YU Yan;WANG Siqintana;TURGEN Baiyila
2008, 1(9): 9-11.
Abstract
(
2028
)
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Two groups of calves (n=5) weaning to 7-month old fed with corn stover(CT) and hay (H) respectively were selected and given mixed concentrate (2kg/d ).Effects of different quality on the digestibility and growth performance of calves during the age of weaning to 7-month were investigated. The results showed that daily feed intake of CT and H groups were 2.25 and 3.81 kg (P<0.01), respectively; dry matter intake (DMI), neutral detergent fiber (NDF), acid detergent fiber (ADF), crude protein (CP) and fat intakes in H group had higher than CFS, its digestibility were 63.6%, 51.2%, 43.5%, 64.3%, 55.6% and 57.5%, 43.3%, 40.4%, 44.4%, 59.4%; besides digestibility of CP in two groups were significantly different (P<0.01). Daily gain in H and CFS groups were significant (0.788 and 0.522 kg/d, P<0.01), body slanting length (18.3 and 9.5 cm) and chest girth were significant (P>0.05), change of heights was no difference. It was concluded that comparing with corn-straw，supplementing grass hay would increase the dry matter intake and nutrient digestibility of calves fed during the age of weaning to 7-month and improve the growth performance, especially development of body slanting length and chest girth.
Effects of Trace Mineral on Meat Quality
HE Ruofang;;LI Shaoyu;JIN Haitao;;WANG Linyi;YAN Mingtian
2008, 1(9): 12-15.
Abstract
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3327
)
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Meat quality has become research focus in animal science and was affected by various factors including gene, feed, transport, slaughter and so on. This review concerning the effects of chromium, manganese, selenium and iron on meat quality.
Effect of High Zinc Diet Long-term Exposure on Growth Performance and Serum Biochemical Indexes in Piglets
HE Xiaojia;CHEN Liang;LI Ying;WANG Xichun;WU Jinjie
2008, 1(9): 17-20.
Abstract
(
1979
)
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To study the effect of high zinc diet long-term exposure on growth performance and serum biochemical indexes, sixty “Duroc×Landrace×Yorkshire” crossbred weanling piglets (23 ±1 days of age) were randomly divided into 3 groups with 20 piglets each by weight and sex, fed with basal diet, basal diet plus 3000 mg/kg zinc (from ZnO), and basal diet plus 500 mg/kg zinc (from ZnMet), respectively. Blood samples in 10 piglets from each group were taken on 0，21，42，70 d after weaning for measuring serum biochemical indexes. The results showed that high zinc diets increased significantly (P<0.05 or P<0.01) absolute weigh(AW) and average daily gain(ADG) of weanling stress piglets on 0~42 d postweaning, and dreased significantly (P<0.05) the average daily gain on 42~70 postweaning. The serum AST and ALT levels were significantly (P<0.05 or P<0.01) higher in piglets of treating group than that in piglets of control group on 42~70 postweaning , and the serum TP and ALB levels were significantly (P<0.05 or P<0.01) lower. High zinc diets increased significantly (P<0.01) ALP levels of weanling stress piglets on 21~70 d postweaning. The results indicated that feeding high zinc diets can remarkably degrade weanling piglets stress on the earlier period, but high-zinc diet longterm exposure to weanling piglets caused poisoning response.
Effect of Small Peptides on Growth Performance and Biochemical Parameters of the Yellowfeathered Broilers
TANG Weitong;LI Jiajia;LIN Xubin;HUANG Guanqing
2008, 1(9): 21-23.
Abstract
(
2046
)
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Sixty-four healthy yellow-feathered broilers of 1-day-old were selected and divided into group A and group B randomly, each group with 4 replicates in which had 8 yellow-feathered broilers. Group A fed the dietⅠ(include 5% fishmeal), group B fed the dietⅡ(include 2% fishmeal) in which added 0.5% small peptides. The trial lasted 8 weeks to investigate the effect of small peptides on growth performance and biochemical parameters of the yellowfeathered broilers. The results showed that used a small number of small peptide products to replace a certain amount of fish meal in the diet reducing dietary protein content, even made yellowfeathered broilers have higher growth performance. Also the content of the serum protein and serum albumin were significantly（P<0.05）increased and the serum total cholesterol were significantly（P<0.05）reduced in the 2-week-old of yellow-feathered broilers.
Effects of Supplement of Youliantai on Growth Performance and Blood Biochemical Parameters in Broiler Chicken with Yellow-feather
LIU Xiaoshen;ZHANG Jiabao;REN Wenzhi;LIU Dianfeng;WEN Lizheng;CHEN Chengzhen
2008, 1(9): 24-27.
Abstract
(
1858
)
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450 one-day cockerels, which were healthy and named north third, were used in this experiment. Those cockerels were divided into six groups (75 cockerels, 3 replications in each group)randomly, Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ,groups were given 0, 20, 40, 60, 80, 100 mg/kg Youliantai respectively. All cockerels were under the same circumstance (temperature, moisture and illumination), automatic drinking water and foraging freedom. At the time of 14, 28, 42, 56, 63 d after beginning experiment, the weight of 5 cockerels from each replication were measured and collected the blood. 3 cockerels from each replication were abataged at 63 d. Results display that 60 mg/kg supplement can improve 13.98 percent body weight compared with control group each day and the ratio of forage to body weight were decreased 3.43 percent (P<0.05). The concentrations of serum neuropeptide Y were correlation with increased supplement of Youliantai. Increased weights every day were positive correlation with serum growth hormone (r=0.712) and with serum IGF-Ⅰ (r=0.812). The ratio of forage to body weight were negative correlation with IGF-Ⅰ (r=-0.796). The performances and economic returns were best at the optimal 60 mg/kg supplement concentration for Youliantai.
生物技术
Adhesion Kinesics of Streptococcus suis Type 2 to PK-15 Cells
JIANG Chenggang;LIU Diqiu;CAI Xuehui
2008, 1(9): 27-29.
Abstract
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2622
)
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The objective of this paper was to study the different invasiveness among
streptococcus suis
type 2 which carried various virulence factors,100 strains of
S suis
were isolated from all over the country. 10 strains of them were
S suis
type 2 by PCR identification, strains with virulence factors were selected to explore the adhevence of them to PK-15 cells.7-4［MRP
+
EF
+
］，8-3［MRP
+
EF
-
］,ZH-1［MRP
-
EF
+
］,and control strains LN-2 ［MRP
－
EF
－
］ were chose in adhesion kinesics test to PK-15 cells.The results indicated that MRP factor was closely related to adhesion of
S suis
type 2 to PK-15 cells.
Establishment of SYBR Green I Real-time PCR Aaasy forDetection of Porcine Circovirus Type 2
ZHANG Jinxiu;CHAO Shengyu;DING Min;LI Gang
2008, 1(9): 30-33.
Abstract
(
2829
)
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Porcine circovirus type 2 ORF2 encodes the capsid protein which contains major immunoreactive epitopes.The whole sequence of the ORF2 was amplified with a special pair of primers and cloned into vectors.The PCV-2 DNA was obtained from the supernatant of PCV-2 infected Dulac cells after freeze thrawing.Then a two-step method of SYBR Green I Real-time PCR amplification was established for the rapid and sensitive detection of PCV2.The detection results confirmed that the established method was rapid, specific and sensitive.
Study on Infectious Molecular Clone of RNA Virus
QIAN Shufeng;HAO Yulan;ZHENG Cuiling
2008, 1(9): 34-37.
Abstract
(
1969
)
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Following the rise and developmemt of reverse genetics technique, it become possible to work with RNA molecule in vitro.The construction infectious molecular clone of RNA virus and operation in DNA moleculear level in vitro is the key to reverse genetics technique. The virus's structure and function can be studied by this way. In this articles the way of constructing infectious clone and in vitro transcription were reviewed.
Cloning and Sequence Analysis of the L1 Gene of Avian Reovirus C-98 Strain
HAO Ruifeng;GUAN Pingyuan;Wurihan;Temuerbagen;MA Lifeng;YU Fuli;LI Ruigang
2008, 1(9): 37-40.
Abstract
(
2644
)
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Three specific pairs of primers, which were designed according to avian reovirus strain S1133 (GenBank) L1 gene sequence, were used to clone the L1 gene of the avian reovirus C-98 isolated from Inner Mongolia through RTPCR. Then the acquired L1 gene was sequenced. The result showed that there was 3959 bp in C-98 L1 gene, including 21 bp of the 5’ noncoding region and 56 bp of 3’ noncoding region. The reading frame was between the 22
nd
bp and the 3903
rd
bp. The comparison of the sequences of different strains showed higher homology between the strain C-98 and strain 919 from Taiwan district, strain 1733, 2408, S1133 from USA, and the homology of nucleotide sequence was 99.8%, 99.7%, 99.7% and 99.4% respectively; while there was a low relationship in comparison with the mammalian reovirus L3 genes and corresponding amino acid sequences, and the homology of nucleotide sequence was between 43% and 45%.
The Progress in Individual Age Estimation by the Length of Telomere
YANG Xiaofeng;YANG Shuming;TANG Xiaoyan;YU Hongxia;WANG Di;SUN Fengmei;
2008, 1(9): 41-44.
Abstract
(
1885
)
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The mechanism of cellular senescence is an important issue in cell biology. With the development of study on relationship between telomeretelomerase and cell senescence/aging, the telomere shortening has become one of the biological marks of cell aging and age growth. This article firstly introduced the structure and function of telomere and telomerase, then analyzed the relationship between them and cell aging or the growth of age, finally summarized the application progress in age estimation based on telomeres and telomerase.
The Prevention of
Blastomyces Albicans
Contamination in Cell Culture
ZHAO Wei;XUE Jianhua
2008, 1(9): 45-47.
Abstract
(
2596
)
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To study the feasibility of preventing
blastomyces albicans
contamination in cell culture by Fluconazole, Fluconazole was added into medium with different concentration，the survival of C2C12 was observed．The results showed that the combination of 300 μg／ml Fluconazole was better than others in preventing
blastomyces albicans
，and it did not obviously affect the survival of C2C12 cells．Conclusion: Fluconazole can be used to eliminate
blastomyces albicans
contamination in cell culture．Fluconazole, Amikacin and Cefradine can be used to prevent eumycete contamination in cell culture．
Expression of β- defensins in Foetal Mongolia Sheep in Situ Hybridization
SHAO Yanhong;LI Yonglan;CAO Guifang;WANG Xiumei;LI Shufeng
2008, 1(9): 48-52.
Abstract
(
1837
)
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Detected the expression of the mRNA of β-defensins (SBD-2) in the foetal Mongolia sheep by in situ hybridization which labeled with digoxin.Took the lung, stomach, intestine, uterus, tongue, heart from the 4-month-old foetal Mongolia sheep to do the situ hybridization. Results: sBD-2 mRNA distributes in lung, stomach, intestine, uterus, tongue, heart which are representative of the respiratory system, digestive system ,absorption system ,reproductive system and muscle tissue of the foetal Mongolia sheep.Indicated that sBD-2 distributed widely in the fetal body as an effective and broad-spectrum antibacterial peptide.
Construction of pGherlin Eukaryotic Expression Plasmid and of its Effect on the Rat Growing
DUAN Xiaoming;FAN Jinxiu;GUO Rui;LI Jiakui
2008, 1(9): 52-55.
Abstract
(
1730
)
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The cDNA of pGhrelin was amplified by RT-PCR and was cloned into the pcDNA3.1 (+) to construct the eukaryotic expression vector. The vector was injected to rats’ hind legs’ muscle. The result showed that rat growth was improved after the injection of vector at 10, 20, 35 d post-injection in the treatment rats was higher (P<0.05) than that of control, but the rat weight at 46 d postinjection in the treatment rats was not obviously improved. It suggests that the eukaryotic expression vector has the ability to promote the growth of rats.
遗传繁育
The Advance of Parthenogenetic Activation of Oocyte on Different Mammals
WU Yunqiqige;LI Xiangchen;YUE Hua;LIU Peng;XI Ninigen;GUAN Weijun;MA Yuehui
2008, 1(9): 55-58.
Abstract
(
1732
)
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The parthenogenetic activation of oocyte is one of the key technologies of nuclear transplantation, the rate of oocyte activation directly affects the efficiency of nuclear transplantation. This paper mainly discusses oocyte parthenogenetic activation on different mammals and its mechanism and paths.
Bovine PON1 Gene and PON2 Gene Cloning and Prokaryotic Expression
JI Aiguo;HUAI Yahong;ZHANG Lupei;LI Junya;WANG Shuhui;XU Shangzhong;GAO Xue;REN Hongyan
2008, 1(9): 59-63.
Abstract
(
2086
)
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To construct and express prokaryotic expression vector of bovine paraoxonase-1 and paraoxonase-2 (PON1 and PON2) and to obtained pure PON1 and PON2. Full length sequences of PON1 and PON2 genes were amplified from bovine liver by RT-PCR and were cloned into the expression vector-pET28a. PON1 and PON2 fusion proteins were expressed in BL21 under IPTG induction and the expressed fusion proteins were detected by SDSPAGE. The recombinant plasmids containing the target genes were constructed successfully. The fusion proteins were expressed in
E coli
in soluble form. The fusion proteins are expressed in soluble form, which is a useful reagent for further preparation of purifying and study of the function of PON1 and PON2 in blood vessel and diabetes mellitus, and boosting the birth weight of calves.
The Molecular Genetic Study on Etiological Factor of Mink Self-Biting
MA Lina;LI Yumei;BAI Xiujuan
2008, 1(9): 64-67.
Abstract
(
2432
)
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Self-biting is the frequent diease to furriery animal such as mink,fox,which affect on the furriery qualities. The reason isn’t definite.Primarily discuss the reason from molecule genetics.Constract the group of health and self-biting with RAPD (random amplified polymorphic DNA，RAPD)，randomly select 40 polymorphisms primers which are good reproducibility. Abstract 40 DNA from each group,and random construction gene cistem from 10 DNA. Primer S103(sequence is AGACGTCCAC) with obviouslily difference from health and selfbiting is selected,and clone,send the result of clone to Sangon biologic company in shanghai to sequence. Through primer S103, only in the disease mink groups we amplify 750 bp DNA fragment, 600 bp DNA is showed up in the health group but disease group isn't. So this can serve as molecular genetic labeling to distinguish from healthy and sick mink groups. So the different fragments both primarily distinguish the molecular genetic labeling from health and diease,it bases on the deeply study for the self-biting.
The Post-translational Modification of Tumor-inhibiting Factor P53
NA Risu;LIU Changqing;LU Taofeng;DAO Erji;GUAN Weijun;MA Yuehui;
2008, 1(9): 68-71.
Abstract
(
785
)
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The tumor-inhibiting factor P53 is most closely related with cancer， and it must be post-translational modified before performing its regular function. The comprehensive realization of the process has great significance in molecular mechanism of tumorigenesis and treatment and prevention of tumour. This article generality discussed several questions about the posttranslational modification of P53 including phosphory-lation modification， acetylation modification， smallubiquitin-like modification and the ubiquitinproteasome pathway of P53， and illuminated its regulatory networks. This discussion supplied the theory basis to have a better realization in the molecular mechanism of treatment and prevention of tumour.
The Research Progress in Genome Imprinting
WANG Jianchun;WU Guojiang;LI Shijie;
2008, 1(9): 71-73.
Abstract
(
1837
)
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Genomic imprinting is a parentoforigin specific gene expression which is a vital epigenetic mechanism. This article reviews the characters of imprinting genes and the mechanisms of genomic imprinting. It also demonstrates the biology significance of genomic imprinting.
Blood Protein Polymorphism and Cluster Analysis of Bos Grunniens in China
GU Chaoyong
2008, 1(9): 74-75.
Abstract
(
1643
)
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Analysing 11 categories of China bos grunniens by using system cluster analysis according to blood protein polymorphism, suggests that China bos grunniens is classified west hypotype and north hypotype. Also the west hypotype is classified Qinhai-Xizang platequ hypotype and traversing mountain hypotype. In addition, China bos grunniens’ consanguinity close, the origin is single. The blood protein polymorphism is comparatively poor, and the gene tends to homozygosity.
Investigation on Polymorphism of Plasma Cerum Loplasmin in Duluoke Hybird-pig
SHI Quanjun;FANG Junbang;LU Fushan
2008, 1(9): 76-77.
Abstract
(
1595
)
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20 Duluoke（paternal） Hybirdpigs were used to investigate the polymorphism of plasma cerumLoplasmin（Cp）by means of a polyacrylamide gel eletrophoresis in vertical plate.The results were as follows： ①There were both CpAB and CpBB phenotypes on the locus of plasma cerumLoplasmin in Duluoke binary and ternary Hybirdpigs examined and CpBB type was a preponderance phenotypes； ②The frequencies of CpA and CpB alleles were 0.1429,0.2308 and 0.8571,0.7692 in Duluoke binary and ternary Hybirdpigs examined; ③The gene heterozygosities were separate 0.2441,0.3350，the gene homozygosities were separate 0.7559,0.6650，the numbers of effective allele were separate 1.3229,1.5038 and the gene homozygosity indexes were separate 0.7152,0.6371.
奶业专栏
The Application of Dairy Ration Formulation Diagnosis System
CHEN Bo;XIONG Benhai;SHAO Changhong
2008, 1(9): 78-80.
Abstract
(
2021
)
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Only sereval important parameters are calculated in the majority of home current dairy cattle ration formulation software programs. This is because it is difficult to consider all nutrients parameters in a whole system. Developed by the author, this dairy cattle ration formulation diagnosis system could compute those reached nutrients in cattle ration and diagnose nutrients banlance according to the ingredients composition on a cattle ration. Also, this software could predict some basic production performance after feeding a specific ration. Limit to the space, this article just takes the banlance of metabolize protein in dairy nutrition as an example to calculate and analyze the protein banlance of dairy ration according to the result calculated by this diagnose software.
Hot Stress with HSP70 Expression in Dairy Cow
GUO Tongjun;WANG Jiaqi;WANG Jianping;BU Dengpan;WEI Hongyang;ZHOU Lingyun
2008, 1(9): 83-86.
Abstract
(
1693
)
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HSP70 is the marked protein and typical protein of heat shock proteins at the stress. This paper summarized the HSP70 class,structure,function,transcription,translation and detection methods.
疾病防治
Pathological Changes of
Tragopan Temminckii
Infected with
Heterakis Isolonche
ZHANG Min;LI Tongyi;LI Dezhong;ZHANG Longxian;CUI Bin;DING Li;WANG Fang;NING Changshen
2008, 1(9): 86-88.
Abstract
(
2187
)
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Gross lesions and histopathological changes were observed in
tragopan temminckii
that infected naturally by
H isolonche
. The results showed that pathological change mainly occurred in the cecum. Gross lesions were characterized as cecum turgescence, thickening, petechial haemorrhages and erosion of the mucosa, and nodules in the serosa. The examination of cecal histological sections were characterized as hemorrhage, diffuse chronic typhlitis with mononuclear cells infiltrate in the lamina propria, necrotic areas with cholesterol clefts in the submucosa, and
H isolonche
worms were arounded with neoplastic nodules.
Research Progress of the Medicine and Vaccine against Avian Influenza Virus
MO Juan;LIU Jianhua;DU Xiangdang;PAN Yushan;ZHANG Sumei;YUAN Li
2008, 1(9): 91-94.
Abstract
(
1993
)
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Avian influenza(AI) affects poultry industry seriously and threatens the health of human beings. The epidemic of AI, especially about domestic and abroad in recent years, the mechanism of AIV, especially the medicine and vaccine for control AIV research progress were summarized.
Monitoring Antibody Titer of PCV-2 in Northwestern Hubei Province
XIONG Jianglin;ZHOU Hualin;WANG Changyi;YANG Xubo;TAN Huaxiang
2008, 1(9): 96-97.
Abstract
(
1570
)
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The antibody titer of PCV-2 of 282 serum samples from 38 standard pig farms were detected with ELISA. The results showed the positive sera were 207, the positive rate was 73.4%,and the positive values were between 0.15 and 0.60, especially focused on 0.3 to 0.4,which was up to 25.1%. Among the tested pig farms, 82.9％ pig farms were PCV-2 positive and only 17.1％ pig farms were negative. Through the clinic observation and the pathologic autopsy, the sick swine had the typical character of PCV-2.
兽医临床
Pathogeny, Diagnosis and Treatment of Canine Urolithiasis
WANG Baojie;CAI Lanfen
2008, 1(9): 114-115.
Abstract
(
1787
)
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Canine urolithiasis was one of the common diseases in veterinary clinics. In this review, pathogeny, diagnosis and treatment of canine urolithiasis were analyzed and summarized , in order to provide reference for prevention and treatment of the disease.
动物生产
Mechanism and Regulation of Heat Stress on Livestock Reproductive Performance
GUO Liang;ZHANG Minhong
2008, 1(9): 119-122.
Abstract
(
1747
)
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Heat stress can affect livestock reproductive performance and animal production. In order to improve prevention measures, the effect，mechanism and regulation of heat stress on livestock reproductive performance are reviewed.
Study of Improvement of Conception Rate in Yellow Cattel and Hybrrd Cattel
ZHENG Hong
2008, 1(9): 132-133.
Abstract
(
1572
)
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To 471 oxen, the mixed stud bull carries on enhances the conception rate the experiment. The result indicated tha the cow sentiment time conception rate and the total conception rate processes which with LRH-A respectively were 56.6% and 65.93%, and were signficantly higher than control group(24.42% and 49.21%)(P<0.05); The cow sentiment time conception rate and the total conception rate processes which with hCG respectively are 53.33% and 72.72%, and were signficantiy higher than those in the cow of control group(33.69% and 52.54%)(P<0.05).
Study of Improvement of Conception Rate in Yellow Cattel and Hybrrd Cattel
ZHENG Hong
2008, 1(9): 133-134.
Abstract
(
1643
)
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Metrics
To 471 oxen, the mixed stud bull carries on enhances the conception rate the experiment. The result indicated that the cow sentiment time conception rate and the total conception rate processes which with LRH-A respectively were 56.69% and 65.93%, and were signficantly higher than control group(24.40% and 49.21%)(P<0.05); The cow sentiment time conception rate and the total conception rate processes which with hCG respectively are 53.33% and 72.72%, and were signficantiy higher than those in the cow of control group(33.69% and 52.54%)(P<0.05).
Produce Performance Canada for Angus and Guizhou Weining Cattle
FANG Hua;WANG Shiyan;LI Xiaojiang;LI Bo;LUO Wei
2008, 1(9): 135-136.
Abstract
(
1670
)
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Angus, adaptability is very capable, the fertility is high. The bull calf was produced by Angus♂×Wei♀, this time the biggest newborn weigh was 33.50 kg, and the smallest was 19.80 kg. Usually, the difference was the biggest notable (P<0.01) than the bull calf’s newborn weigh “10 to 15 kg” by Weining cattle self mating and animal husbandry calving.
经验交流
GPI: Important Role for Prion Toxicity
ZHANG Taixiang;TIAN Guoning;ZHANG Jinling;TIAN Guohua
2008, 1(9): 141-142.
Abstract
(
2318
)
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Prion was nerve recessive encephalopathy of human and other mammal, which was one of the hot point in the world. It was said the pathogenic of the disease was the protein, which had same first structure with the normal protein coded by the host, just had different conformation of the second structure. Now it is not clear for the mechanism of the disease. So the author reviews the relation of the prion toxicity and GPI according to the report of other people who research the prion.
Protection and Utilization of Breeds of Bamei Pig
YE Zhanshun
2008, 1(9): 148-150.
Abstract
(
1658
)
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Bamei pig, named Jingchuan pig or Huzhu pig, is a famous local breed in china, and it has the features of strong adaptability, high reproduction, good taste, steadily heritage, etc. However, for some reasons, the quantity of Bamei pig reduces sharply and it nearly dies out in the recent years. This paper is proposed to provide the theory and the technology support for the utilization of Bamei pig in china. The purpose is to enhance the seed selective breeding dynamics, enlarge the raw-breeding flock, find out the best way of protecting the breeds; carry out carry out “field + peasant household” production commodity mixed superior pig's breeding promotion pattern; and discuss the development prospect of the “high quality, security, green” pork.
Discussion about Reducing Digestive Time of Kjeldahl Determination
ZHANG Houfeng;ZHANG Shuping
2008, 1(9): 157-158.
Abstract
(
1585
)
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Metrics
Hydrogen peroxide was used in digesting of Kjeldahl determination, the digestive time of Kjeldahl determination reduced from 2.5~3 h to 1 h. The testing results indicated that Kjeldahl determination is fast and simple, accurate and exact.
