检测猪圆环病毒2型SYBR Green I荧光定量PCR方法的建立

›› 2008, Vol. 1 ›› Issue (9): 30-33.

• 生物技术 • Previous Articles Next Articles

Establishment of SYBR Green I Real-time PCR Aaasy forDetection of Porcine Circovirus Type 2

ZHANG Jinxiu1，CHAO Shengyu1，2，DING Min1，LI Gang1

1. Institute of Animal Science，Chinese Academy of Agricultural Sciences， Beijing 100193，China；2.Institute of Animal Sciences，Haixi Autonomous Prefecture,Delingha 817000,China

Received:1900-01-01 Revised:1900-01-01 Online:2008-09-20 Published:2008-09-20

Abstract

Abstract: Porcine circovirus type 2 ORF2 encodes the capsid protein which contains major immunoreactive epitopes.The whole sequence of the ORF2 was amplified with a special pair of primers and cloned into vectors.The PCV-2 DNA was obtained from the supernatant of PCV-2 infected Dulac cells after freeze thrawing.Then a two-step method of SYBR Green I Real-time PCR amplification was established for the rapid and sensitive detection of PCV2.The detection results confirmed that the established method was rapid, specific and sensitive.

Key words: porcine circovirus type 2; Dulac cells; SYBR Green I Real-time PCR

CLC Number:

S852.65+9.2

ZHANG Jinxiu;CHAO Shengyu;DING Min;LI Gang. Establishment of SYBR Green I Real-time PCR Aaasy forDetection of Porcine Circovirus Type 2 [J]. , 2008, 1(9): 30-33.

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Establishment of SYBR Green I Real-time PCR Aaasy forDetection of Porcine Circovirus Type 2

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