›› 2008, Vol. 1 ›› Issue (9): 37-40.

• 生物技术 • Previous Articles     Next Articles

Cloning and Sequence Analysis of the L1 Gene of Avian Reovirus C-98 Strain

HAO Ruifeng1, GUAN Pingyuan1, Wurihan2, Temuerbagen2, MA Lifeng2, YU Fuli2, LI Ruigang2
   

  1. 1.College of Animal Science and Animal Medicine, Inner Mongolia Agricultural University, Huhhot 010018, China; 2.Center of Prevent and Control of Animal Disease of Inner Mongolia, Huhhot 010010, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-09-20 Published:2008-09-20

Abstract: Three specific pairs of primers, which were designed according to avian reovirus strain S1133 (GenBank) L1 gene sequence, were used to clone the L1 gene of the avian reovirus C-98 isolated from Inner Mongolia through RTPCR. Then the acquired L1 gene was sequenced. The result showed that there was 3959 bp in C-98 L1 gene, including 21 bp of the 5’ noncoding region and 56 bp of 3’ noncoding region. The reading frame was between the 22nd bp and the 3903rd bp. The comparison of the sequences of different strains showed higher homology between the strain C-98 and strain 919 from Taiwan district, strain 1733, 2408, S1133 from USA, and the homology of nucleotide sequence was 99.8%, 99.7%, 99.7% and 99.4% respectively; while there was a low relationship in comparison with the mammalian reovirus L3 genes and corresponding amino acid sequences, and the homology of nucleotide sequence was between 43% and 45%.

Key words: avian reovirus; L1 gene; molecular cloning; sequence analysis

CLC Number: