禽呼肠孤病毒感染DF1细胞后干扰素刺激基因在转录水平表达量的动态变化

doi:10.16431/j.cnki.1671-7236.2021.04.030

Abstract

Abstract: In order to reveal the expression of interferon (IFN) and interferon stimulated gene in DF1 cells infected with Avian reovirus (ARV),infect DF1 cells with ARV virus,observe cytopathic changes,collect cell samples at 0,6,12,24,36,48,72 and 96 h after infection,and extract reverse transcription into cDNA.Real-time PCR assays was used to detect the dynamic changes of interferon IFN-α and IFN-β and 9 common avian interferon-stimulating genes at the transcription level at different time points after infection.The results showed that after ARV infected DF1 cells,DF1 cells appeared typical cytopathic effect.The virus began to proliferate rapidly at 12 h after infection and maintained at a high level from 36-96 h.The expression of IFN-α and IFN-β at the transcriptional level was significantly down-regulated after infection (P<0.05;P<0.01);IFI6,OAS,IFIT5,and ISG12 had similar changes in the expression level of transcription,and all showed significant up-regulation (P<0.05;P<0.01),reaching peak at 96 h after infection.Among them,IFIT5 had the largest up-regulation,and its expression at 96 h after infection was 19.62 times than that at 0 h (P<0.01).While the expression changes of Mx,IFITM3,PKR,Viperin,and ZAP were similar,and all showed significant down-regulation (P<0.05;P<0.01),among them,the downward adjustment of Mx,IFITM3 and Viperin was larger than that of PKR and ZAP.The results showed that interferon and various interferon-stimulating genes showed regular changes at the transcription level after ARV infection in DF1 cells,which was related to virus replication in DF1 cells.The results showed that ARV infection could induce the expression of variety of interferon-stimulating genes,which played an important role in resisting ARV virus invasion,inhibiting ARV virus replication,release,and virus clearance.This study provided a further research direction of ARV pathogenesis and virus-inhibiting mechanism responses to ARV infection.

Key words: Avian reovirus (ARV); DF1 cell; interferon; interferon stimulated gene; transcrip-tional level

CLC Number:

S831.7

WANG Sheng, WAN Lijun, XIE Zhixun, XIE Liji, LUO Sisi, FAN Qing, ZHANG Yanfang, ZENG Tingting, HUANG Jiaoling, ZHANG Mingxiu, XIE Zhiqin, DENG Xianwen. Altered Gene Expression Profiles of the Interferon Stimulated Genes in DF1 Cells Infected with Avian Reovirus[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1423-1430.

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Altered Gene Expression Profiles of the Interferon Stimulated Genes in DF1 Cells Infected with Avian Reovirus

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