精氨酸对干扰素-tau处理牛子宫内膜上皮细胞基因表达的影响

doi:10.16431/j.cnki.1671-7236.2021.07.025

Abstract

Abstract: This experiment was aimed to investigate the regulatory effect of arginine(Arg) on bovine endometrium treated with interferon-tau(IFNT) and its potential molecular mechanism.Bovine endometrial epithelial cells were inoculated into 6-well cell culture plate,different concentrations of IFNT (0,100 ng/mL) were added into the culture medium after the confluence of cells reached 70% to 80%.Cells were harvested to determine the expression of genes related to unfolded protein response (UPR) pathway after 12 h;Different concentrations of Arg (0,0.05,0.1,0.2,0.5,1,2 mmol/L) were subjected to bovine endometrial epithelial cells to detect the proliferation rate for 24 h by CCK8 method,and the optimal concentration of Arg was selected;Bovine endometrial epithelial cells were randomly divided into Arg starvation group,Arg starvation+IFNT treatment group,Arg+IFNT treatment group,100 ng/mL IFNT was added after Arg deprivation or supplementation for 6 h.The expression of genes associated with UPR pathway (BIP,PERK,CHOP,ATF6),apoptosis (Bax,Caspase9) and endometrial receptivity (HOXA10) were detected after IFNT stimulation for 12 h.The results showed that:IFNT stimulation significantly up-regulated the expression of BiP,PERK,CHOP and ATF6 genes implicated in UPR pathway in bovine endometrial epithelial cells.The proliferation rate of bovine endometrial epithelial cells were significantly increased by 0.5 mmol/L Arg (P<0.05);Arg deprivation significantly up-regulated the mRNA level of Bip,PERK,CHOP,ATF6 and BAX genes in IFNT-treated bovine endometrial epithelial cells (P<0.05),and significantly down-regulated the mRNA level of HOXA10 (P<0.05),but had no significant effect on the expression of Caspase9 mRNA (P>0.05).The results indicated that Arg deficiency could lead to endoplasmic reticulum stress in bovine endometrial epithelial cells and influence the establishment of endometrial receptivity,which could provide reference for further revealing the regulation mechanism of Arg on bovine endometrium and formulating nutritional regulation strategies to reduce embryo loss in early pregnancy.

Key words: arginine (Arg); interferon-tau; bovine endometrial epithelial cell; unfolded protein response

CLC Number:

S814.8

YU Jie, HU Xiuzhong, XIANG Min, WANG Dingfa, TAO Bifei, XIA Yu, TAO Hu, XIONG Qi, CHENG Lei. Effects of Arginine on Expression of Genes in Bovine Endometrial Epithelial Cells Treated with Interferon-tau[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2495-2503.

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Effects of Arginine on Expression of Genes in Bovine Endometrial Epithelial Cells Treated with Interferon-tau

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