日粮精氨酸水平对蛋鸭卵泡膜血管形成相关基因及蛋白表达的影响

doi:10.16431/j.cnki.1671-7236.2018.02.012

Abstract

Abstract:

This study was aimed to evaluate the effects of dietary arginine (Arg) levels on angiopoiesis related genes and proteins expression in the follicle membranes of laying ducks. 396 healthy Longyan laying ducks at 15 weeks of age with similar body weight were randomly assigned into 3 groups with 6 replicates per group and 22 birds per replicate. All laying ducks were offered a basal diet (0.66% Arg) without Arg supplementation for 2 weeks. And then were fed the experimental diets supplemented with 0,0.4% and 0.8% Arg in the form of L-arginine hydrochloride (L-Arg·HCl),which contained 0.66%,1.06% and 1.46% Arg,respectively. The experiment lasted for 17 weeks. The results showed as follows:①The LDL-C and NO concentration of laying ducks in 1.06% and 1.46% Arg groups were significantly higher than that in 0.66% Arg group (P < 0.05).②Compared to the 0.66% Arg group, the mRNA abundance of the homeodomain-interacting protein kinase 2 (HIPK2) were significantly increased in the F2 large yellow follicle membranes of laying ducks fed diets with 1.06% and 1.46% Arg (P < 0.05). The mRNA abundance of angiogenin 1 (Ang1) in the F2 large yellow follicle membranes of 1.46% Arg group was significantly higher than that in 0.66% and 1.06% Arg groups (P < 0.05).③The protein abundance of the HIPK2 and transforming growth factor β1 (TGFβ1) in the F2 large yellow follicle membranes of laying ducks fed diets with 1.06% and 1.46% Arg were significantly higher than those fed 0.66% Arg (P < 0.05). In conclusion,dietary Arg level of 1.46% that achieved by supplementing the basal diet with 0.8% L-Arg·HCl could improve angiopoiesis probably by activating HIPK2 and TGFβ1 in TGFβ signaling pathway,and increase blood flow to facilitate vitellogenesis in the large yellow follicles of Longyan laying ducks.

Key words: arginine (Arg); Longyan laying ducks; follicle; vitellogenesis; angiopoiesis

CLC Number:

Q786

XIA Weiguang, CHEN Wei, RUAN Dong, WANG Shuang, FOUAD A M, LUO Xi, XIONG Yunxia, WANG Shenglin, ZHENG Chuntian. Effects of Dietary Arg Levels on Angiopoiesis Related Genes and Proteins Expression in the Follicular Membranes of Laying Ducks[J]. , 2018, 45(2): 384-391.

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Effects of Dietary Arg Levels on Angiopoiesis Related Genes and Proteins Expression in the Follicular Membranes of Laying Ducks

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